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CryoEM structure of beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (Class O)

Electron Microscopy
3.8Å resolution
PDB entry 8unz coloured by chain, front view.
PDB entry 8unz coloured by chain, side view.
PDB entry 8unz coloured by chain, top view.
Source organism: Homo sapiens
Assembly composition:
hetero tetramer (preferred)
Entry contents: 4 distinct polypeptide molecules

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Assemblies

Assembly 1 (preferred)
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Multimeric state: hetero tetramer
Accessible surface area: 42065.81 Å2
Buried surface area: 7996.48 Å2
Dissociation area: 987.85 Å2
Dissociation energy (ΔGdiss): 1.7 kcal/mol
Dissociation entropy (TΔSdiss): 13.93 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-272389

Macromolecules

Guanine nucleotide-binding protein G(s) subunit alpha isoforms short
Chain: A
Length: 380 amino acids
Theoretical weight: 44.33 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P63092 (Residues: 1-394; Coverage: 96%)
  • Best match: P63092-2 (Residues: 1-380)
Gene names: GNAS, GNAS1, GSP
Pfam: G-protein alpha subunit
Guanine nucleotide-binding protein G(s) subunit alpha isoforms short in PDB entry 8unz, assembly 1, front view.
Guanine nucleotide-binding protein G(s) subunit alpha isoforms short in PDB entry 8unz, assembly 1, side view.
Guanine nucleotide-binding protein G(s) subunit alpha isoforms short in PDB entry 8unz, assembly 1, top view.
PDBe-KB: UniProt Coverage View: P63092  
138050100150200250300350
 
100200300
UniProt
P63092
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Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1
Chain: B
Length: 343 amino acids
Theoretical weight: 37.57 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P62873 (Residues: 2-340; Coverage: 100%)
Gene name: GNB1
Pfam: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 in PDB entry 8unz, assembly 1, front view.
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 in PDB entry 8unz, assembly 1, side view.
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 in PDB entry 8unz, assembly 1, top view.
PDBe-KB: UniProt Coverage View: P62873  
134350100150200250300
 
100200300
UniProt
P62873
Chains
Secondary structure
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Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2
Chain: G
Length: 71 amino acids
Theoretical weight: 7.86 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P59768 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Pfam: GGL domain
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 in PDB entry 8unz, assembly 1, front view.
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 in PDB entry 8unz, assembly 1, side view.
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 in PDB entry 8unz, assembly 1, top view.
PDBe-KB: UniProt Coverage View: P59768  
171510152025303540455055606570
 
204060MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENPFREKKFFCAIL
UniProt
P59768
Chains
Secondary structure
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Beta-2 adrenergic receptor
Chain: R
Length: 460 amino acids
Theoretical weight: 51.77 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P07550 (Residues: 1-24, 25-365, 366-413; Coverage: 100%)
Gene names: ADRB2, ADRB2R, B2AR
Pfam: 7 transmembrane receptor (rhodopsin family)
Beta-2 adrenergic receptor in PDB entry 8unz, assembly 1, front view.
Beta-2 adrenergic receptor in PDB entry 8unz, assembly 1, side view.
Beta-2 adrenergic receptor in PDB entry 8unz, assembly 1, top view.
PDBe-KB: UniProt Coverage View: P07550  
146050100150200250300350400450
 
200400
UniProt
P07550
Chains
Secondary structure
Flexibility predictions
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