› 8uy2

X-ray diffraction
2.83Å resolution

Methylenetetrahydrofolate reductase from Chaetomium thermophilum DSM 1495, AdoMet-bound, Inhibited (T) State

Released:
DOI: 10.2210/pdb8uy2/pdb
PDB entry 8uy2 coloured by chain, front view.
PDB entry 8uy2 coloured by chain, side view.
PDB entry 8uy2 coloured by chain, top view.
Source organism: Chaetomium thermophilum var. thermophilum DSM 1495
Primary publication:
Structural basis of S-adenosylmethionine-dependent allosteric transition from active to inactive states in methylenetetrahydrofolate reductase.
Yamada K, Mendoza J, Koutmos M
OpenAccess logo Nat Commun 15 5167 (2024)
PMID: 38886362

Function and Biology Details

Reaction catalysed: 
5-methyltetrahydrofolate + NAD(P)(+) = 5,10-methylenetetrahydrofolate + NAD(P)H
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-245995 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
MTHFR SAM-binding regulatory domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 617 amino acids
Theoretical weight: 70.26 KDa
Source organism: Chaetomium thermophilum var. thermophilum DSM 1495
Expression system: Escherichia coli
UniProt:
  • Canonical: G0S5U9 (Residues: 1-614; Coverage: 100%)
Gene name: CTHT_0033700
Sequence domains:

Ligands and Environments


Cofactor: Ligand FAD 4 x FAD
5 bound ligands:
Ligand SAM 8 x SAM
Ligand ACT 4 x ACT
Ligand SO4 9 x SO4
Ligand NA 1 x NA
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Unit cell:
a: 130.656Å b: 149.948Å c: 171.056Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.188 0.187 0.203
Expression system: Escherichia coli

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