› 8w4m

X-ray diffraction
2.18Å resolution

Crystal structure of open conformation of human immunoglobulin Fc in presence of EndoSz

Released:
DOI: 10.2210/pdb8w4m/pdb
PDB entry 8w4m coloured by chain, front view.
PDB entry 8w4m coloured by chain, side view.
PDB entry 8w4m coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure-Based High-Efficiency Homogeneous Antibody Platform by Endoglycosidase Sz Provides Insights into Its Transglycosylation Mechanism.
Hsieh YC, Guan HH, Lin CC, Huang TY, Chuankhayan P, Chen NC, Wang NH, Hu PL, Tsai YC, Huang YC, Yoshimura M, Lin PJ, Hsieh YH, Chen CJ
JACS Au 4 2130-2150 (2024)
PMID: 38938812

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-246605 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Immunoglobulin gamma-1 heavy chain Chain: A
Molecule details ›
Chain: A
Length: 223 amino acids
Theoretical weight: 25.13 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P0DOX5 (Residues: 227-449; Coverage: 50%)
Sequence domains: Immunoglobulin C1-set domain

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN, GAL, FUC
Carbohydrate polymer
1 bound ligand:
Ligand ZN 10 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE TPS 05A
Spacegroup: C2221
Unit cell:
a: 50.088Å b: 147.668Å c: 75.198Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.21 0.208 0.255
Expression system: Cricetulus griseus

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