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Cryo-EM structure of the ZH8651-bound mTAAR1-Gs complex

Electron Microscopy
3.1Å resolution
PDB entry 8wc4 coloured by chain, front view.
PDB entry 8wc4 coloured by chain, side view.
PDB entry 8wc4 coloured by chain, top view.
Source organisms:
Assembly composition:
hetero pentamer (preferred)
Entry contents: 5 distinct polypeptide molecules

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Assemblies

Assembly 1 (preferred)
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Multimeric state: hetero pentamer
Accessible surface area: 50468.29 Å2
Buried surface area: 10545.93 Å2
Dissociation area: 821.69 Å2
Dissociation energy (ΔGdiss): -4.49 kcal/mol
Dissociation entropy (TΔSdiss): 13.39 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-273940

Macromolecules

Guanine nucleotide-binding protein G(i) subunit alpha-1; Guanine nucleotide-binding protein G(s) subunit alpha isoforms short
Chain: A
Length: 362 amino acids
Theoretical weight: 42.01 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P63096 (Residues: 1-19, 61-181, 229-242; Coverage: 41%)
  • Canonical: P63092 (Residues: 27-67, 205-251, 266-394; Coverage: 55%)
    • nullnull
Gene names: GNAI1, GNAS, GNAS1, GSP
Pfam: G-protein alpha subunit
Guanine nucleotide-binding protein G(i) subunit alpha-1 in PDB entry 8wc4, assembly 1, front view.
Guanine nucleotide-binding protein G(i) subunit alpha-1 in PDB entry 8wc4, assembly 1, side view.
Guanine nucleotide-binding protein G(i) subunit alpha-1 in PDB entry 8wc4, assembly 1, top view.
PDBe-KB: UniProt Coverage View: P63096   P63092  
136250100150200250300350
 
100200300
UniProt
P63096
P63092
Chains
Secondary structure
Interaction interfaces
Sequence conservation
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Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1
Chain: B
Length: 345 amino acids
Theoretical weight: 37.92 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P62873 (Residues: 2-340; Coverage: 100%)
Gene name: GNB1
Pfam: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 in PDB entry 8wc4, assembly 1, front view.
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 in PDB entry 8wc4, assembly 1, side view.
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 in PDB entry 8wc4, assembly 1, top view.
PDBe-KB: UniProt Coverage View: P62873  
134550100150200250300
 
100200300
UniProt
P62873
Chains
Secondary structure
Interaction interfaces
Sequence conservation
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Trace amine-associated receptor 1
Chain: R
Length: 332 amino acids
Theoretical weight: 37.66 KDa
Source organism: Mus musculus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q923Y8 (Residues: 1-332; Coverage: 100%)
Gene names: Ta1, Taar1, Tar1, Trar1
Pfam: 7 transmembrane receptor (rhodopsin family)
Trace amine-associated receptor 1 in PDB entry 8wc4, assembly 1, front view.
Trace amine-associated receptor 1 in PDB entry 8wc4, assembly 1, side view.
Trace amine-associated receptor 1 in PDB entry 8wc4, assembly 1, top view.
PDBe-KB: UniProt Coverage View: Q923Y8  
133250100150200250300
 
100200300
UniProt
Q923Y8
Chains
Secondary structure
Ligand binding sites
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scFv16
Chain: S
Length: 285 amino acids
Theoretical weight: 30.36 KDa
Source organism: synthetic construct
Expression system: Spodoptera frugiperda
scFv16 in PDB entry 8wc4, assembly 1, front view.
scFv16 in PDB entry 8wc4, assembly 1, side view.
scFv16 in PDB entry 8wc4, assembly 1, top view.
128520406080100120140160180200220240260280
 
100200
Chains
Chain D (auth S)
Secondary structure
Interaction interfaces
Sequence conservation
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Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2
Chain: Y
Length: 71 amino acids
Theoretical weight: 7.86 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P59768 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Pfam: GGL domain
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 in PDB entry 8wc4, assembly 1, front view.
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 in PDB entry 8wc4, assembly 1, side view.
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 in PDB entry 8wc4, assembly 1, top view.
PDBe-KB: UniProt Coverage View: P59768  
171510152025303540455055606570
 
204060MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENPFREKKFFCAIL
UniProt
P59768
Chains
Secondary structure
Interaction interfaces
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