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Cryo-EM structure of DSR2-DSAD1

Electron Microscopy
3.4Å resolution
PDB entry 8wkn coloured by chain, front view.
PDB entry 8wkn coloured by chain, side view.
PDB entry 8wkn coloured by chain, top view.
Source organism: Bacillus subtilis
Assembly composition:
hetero pentamer (preferred)
Entry contents: 2 distinct polypeptide molecules

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Assemblies

Assembly 1 (preferred)
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Assembly Name: SPbeta prophage-derived uncharacterized protein YotI and SIR2-like domain-containing protein
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Multimeric state: hetero pentamer
Accessible surface area: 118172.71 Å2
Buried surface area: 18047.57 Å2
Dissociation area: 1,917.51 Å2
Dissociation energy (ΔGdiss): 5.73 kcal/mol
Dissociation entropy (TΔSdiss): 12.75 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-270178

Macromolecules

SIR2-like domain-containing protein
Chains: A, B, C, D
Length: 1005 amino acids
Theoretical weight: 118.64 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli
UniProt:
  • Canonical: D4G637 (Residues: 1-1005; Coverage: 100%)
Gene name: BSNT_07056
Pfam: SIR2-like domain
SIR2-like domain-containing protein in PDB entry 8wkn, assembly 1, front view.
SIR2-like domain-containing protein in PDB entry 8wkn, assembly 1, side view.
SIR2-like domain-containing protein in PDB entry 8wkn, assembly 1, top view.
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SPbeta prophage-derived uncharacterized protein YotI
Chain: E
Length: 120 amino acids
Theoretical weight: 13.87 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q796A8 (Residues: 1-120; Coverage: 100%)
Gene names: BSU19870, yodW, yokF, yotI
SPbeta prophage-derived uncharacterized protein YotI in PDB entry 8wkn, assembly 1, front view.
SPbeta prophage-derived uncharacterized protein YotI in PDB entry 8wkn, assembly 1, side view.
SPbeta prophage-derived uncharacterized protein YotI in PDB entry 8wkn, assembly 1, top view.
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