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Cryo-EM structure of CUL2-RBX1-ELOB-ELOC-FEM1B bound with the C-degron of CUX1 (conformation 1)

Electron Microscopy
3.38Å resolution
PDB entry 8wqe coloured by chain, front view.
PDB entry 8wqe coloured by chain, side view.
PDB entry 8wqe coloured by chain, top view.
Source organism: Homo sapiens
Assembly composition:
hetero dodecamer (preferred)
Entry contents: 6 distinct polypeptide molecules

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Assemblies

Assembly 1 (preferred)
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Multimeric state: hetero dodecamer
Accessible surface area: 142831.86 Å2
Buried surface area: 29776.73 Å2
Dissociation area: 279.33 Å2
Dissociation energy (ΔGdiss): -0.45 kcal/mol
Dissociation entropy (TΔSdiss): 5.09 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-274097

Macromolecules

Cullin-2
Chains: A, C
Length: 750 amino acids
Theoretical weight: 87.11 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q13617 (Residues: 2-745; Coverage: 98%)
Gene name: CUL2
Pfam:
Cullin-2 in PDB entry 8wqe, assembly 1, front view.
Cullin-2 in PDB entry 8wqe, assembly 1, side view.
Cullin-2 in PDB entry 8wqe, assembly 1, top view.
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Protein fem-1 homolog B
Chains: B, D
Length: 627 amino acids
Theoretical weight: 70.36 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UK73 (Residues: 1-627; Coverage: 100%)
Gene names: F1AA, FEM1B, KIAA0396
Pfam: Ankyrin repeats (3 copies)
Protein fem-1 homolog B in PDB entry 8wqe, assembly 1, front view.
Protein fem-1 homolog B in PDB entry 8wqe, assembly 1, side view.
Protein fem-1 homolog B in PDB entry 8wqe, assembly 1, top view.
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Elongin-C
Chains: E, G
Length: 96 amino acids
Theoretical weight: 10.84 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q15369 (Residues: 17-112; Coverage: 86%)
Gene names: ELOC, TCEB1
Pfam: Skp1 family, tetramerisation domain
Elongin-C in PDB entry 8wqe, assembly 1, front view.
Elongin-C in PDB entry 8wqe, assembly 1, side view.
Elongin-C in PDB entry 8wqe, assembly 1, top view.
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Elongin-B
Chains: F, H
Length: 118 amino acids
Theoretical weight: 13.15 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q15370 (Residues: 1-118; Coverage: 100%)
Gene names: ELOB, TCEB2
Pfam: Ubiquitin family
Elongin-B in PDB entry 8wqe, assembly 1, front view.
Elongin-B in PDB entry 8wqe, assembly 1, side view.
Elongin-B in PDB entry 8wqe, assembly 1, top view.
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E3 ubiquitin-protein ligase RBX1, N-terminally processed
Chains: I, J
Length: 96 amino acids
Theoretical weight: 11.2 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P62877 (Residues: 16-108; Coverage: 86%)
Gene names: RBX1, RNF75, ROC1
Pfam: RING-H2 zinc finger domain
E3 ubiquitin-protein ligase RBX1, N-terminally processed in PDB entry 8wqe, assembly 1, front view.
E3 ubiquitin-protein ligase RBX1, N-terminally processed in PDB entry 8wqe, assembly 1, side view.
E3 ubiquitin-protein ligase RBX1, N-terminally processed in PDB entry 8wqe, assembly 1, top view.
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Protein CASP
Chains: K, L
Length: 31 amino acids
Theoretical weight: 3.14 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q13948 (Residues: 656-678; Coverage: 3%)
Gene names: CUTL1, CUX1
Protein CASP in PDB entry 8wqe, assembly 1, front view.
Protein CASP in PDB entry 8wqe, assembly 1, side view.
Protein CASP in PDB entry 8wqe, assembly 1, top view.
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