8wuv

Electron Microscopy
3Å resolution

SpCas9-MMLV RT-pegRNA-target DNA complex (elongation 16-nt)

Released:

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-245025 (preferred)
Entry contents:
2 distinct polypeptide molecules
3 distinct DNA molecules
1 distinct RNA molecule
Macromolecules (6 distinct):
CRISPR-associated endonuclease Cas9/Csn1 Chain: A
Molecule details ›
Chain: A
Length: 1367 amino acids
Theoretical weight: 158.46 KDa
Source organism: Streptococcus pyogenes serotype M1
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: Q99ZW2 (Residues: 2-1368; Coverage: 100%)
Gene names: SPy_1046, cas9, csn1
Sequence domains:
Gag-Pol polyprotein Chain: E
Molecule details ›
Chain: E
Length: 496 amino acids
Theoretical weight: 55.66 KDa
Source organism: Moloney murine leukemia virus
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: Q8UN00 (Residues: 660-1155; Coverage: 29%)
Gene name: gag-pro-pol
Sequence domains:
DNA (51-MER) Chain: C
Molecule details ›
Chain: C
Length: 51 nucleotides
Theoretical weight: 15.64 KDa
DNA (5'-D(*TP*GP*AP*TP*GP*GP*CP*AP*GP*AP*GP*TP*AP*CP*TP*AP*G)-3') Chain: D
Molecule details ›
Chain: D
Length: 17 nucleotides
Theoretical weight: 5.29 KDa
DNA (50-MER) Chain: F
Molecule details ›
Chain: F
Length: 50 nucleotides
Theoretical weight: 15.44 KDa
RNA (137-MER) Chain: B
Molecule details ›
Chain: B
Length: 137 nucleotides
Theoretical weight: 44.3 KDa

Ligands and Environments

No bound ligands
1 modified residue:

Experiments and Validation Details

Entry percentile scores
Resolution:
Relevant EMDB volumes: EMD-37861
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'