› 8x6f

Electron Microscopy
3.7Å resolution

Cryo-EM structure of Staphylococcus aureus sigA-dependent RNAP-promoter open complex

Released:
DOI: 10.2210/pdb8x6f/pdb
PDB entry 8x6f coloured by chain, front view.
PDB entry 8x6f coloured by chain, side view.
PDB entry 8x6f coloured by chain, top view.
Source organism: Staphylococcus aureus
Primary publication:
Structural basis of promoter recognition by Staphylococcus aureus RNA polymerase.
Yuan L, Liu Q, Xu L, Wu B, Feng Y
Nat Commun 15 4850 (2024)
PMID: 38844782
Related structures: EMD-38087

Function and Biology Details

Reaction catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero nonamer (preferred)
PDBe Complex ID:
PDB-CPX-244912 (preferred)
Entry contents:
6 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (8 distinct):
DNA-directed RNA polymerase subunit alpha Chains: A, B
Molecule details ›
Chains: A, B
Length: 314 amino acids
Theoretical weight: 35.05 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q2FW32 (Residues: 1-314; Coverage: 100%)
Gene names: SAOUHSC_02485, rpoA
Sequence domains:
DNA-directed RNA polymerase subunit beta Chain: C
Molecule details ›
Chain: C
Length: 1183 amino acids
Theoretical weight: 133.38 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli
UniProt:
  • Canonical: P47768 (Residues: 1-1183; Coverage: 100%)
Gene names: SAOUHSC_00524, rpoB
Sequence domains:
DNA-directed RNA polymerase subunit beta' Chain: D
Molecule details ›
Chain: D
Length: 1207 amino acids
Theoretical weight: 135.59 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q2G0N5 (Residues: 1-1207; Coverage: 100%)
Gene names: SAOUHSC_00525, rpoC
Sequence domains:
DNA-directed RNA polymerase subunit omega Chain: F
Molecule details ›
Chain: F
Length: 72 amino acids
Theoretical weight: 8.16 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q2G1T9 (Residues: 1-72; Coverage: 100%)
Gene names: SAOUHSC_01177, rpoZ
Sequence domains: RNA polymerase Rpb6
DNA-directed RNA polymerase subunit epsilon Chain: G
Molecule details ›
Chain: G
Length: 72 amino acids
Theoretical weight: 8.76 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q2FZG8 (Residues: 1-72; Coverage: 100%)
Gene names: SAOUHSC_01036, rpoY
Sequence domains: RNA polymerase epsilon subunit
RNA polymerase sigma factor SigA Chain: E
Molecule details ›
Chain: E
Length: 368 amino acids
Theoretical weight: 42.22 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A0J0 (Residues: 1-368; Coverage: 100%)
Gene names: SAOUHSC_01662, plaC, rpoD, sigA
Sequence domains:
DNA (71-mer) Chain: N
Molecule details ›
Chain: N
Length: 71 nucleotides
Theoretical weight: 21.92 KDa
DNA (71-mer) Chain: T
Molecule details ›
Chain: T
Length: 71 nucleotides
Theoretical weight: 21.8 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.7Å
Relevant EMDB volumes: EMD-38087
Expression system: Escherichia coli

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