› 8xvi

Electron Microscopy
3.32Å resolution

Cryo-EM structure of ETAR bound with Endothelin1

Released:
DOI: 10.2210/pdb8xvi/pdb
PDB entry 8xvi coloured by chain, front view.
PDB entry 8xvi coloured by chain, side view.
PDB entry 8xvi coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis of antagonist selectivity in endothelin receptors.
Hou J, Liu S, Zhang X, Tu G, Wu L, Zhang Y, Yang H, Li X, Liu J, Jiang L, Tan Q, Bai F, Liu Z, Miao C, Hua T, Luo Z
Cell Discov 10 79 (2024)
PMID: 39075075
Related structures: EMD-38705

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-276018 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
Guanine nucleotide-binding protein G(s) subunit alpha isoforms short Chain: A
Molecule details ›
Chain: A
Length: 261 amino acids
Theoretical weight: 30.46 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P63092 (Residues: 5-394; Coverage: 63%)
  • Best match: P63092-2 (Residues: 5-380)
Gene names: GNAS, GNAS1, GSP
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 346 amino acids
Theoretical weight: 38.05 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P62873 (Residues: 2-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G
Molecule details ›
Chain: G
Length: 71 amino acids
Theoretical weight: 7.86 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P59768 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain
Nanobody 35 Chain: N
Molecule details ›
Chain: N
Length: 157 amino acids
Theoretical weight: 17.06 KDa
Source organism: Lama glama
Expression system: Escherichia coli
Endoglucanase H; Endothelin-1 receptor Chain: R
Molecule details ›
Chain: R
Length: 683 amino acids
Theoretical weight: 77.88 KDa
Source organisms: Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P16218 (Residues: 26-304; Coverage: 30%)
  • Canonical: P25101 (Residues: 50-405; Coverage: 88%)
Gene names: Cthe_1472, EDNRA, ETA, ETRA, celH
Sequence domains:
Endothelin-1 Chain: T
Molecule details ›
Chain: T
Length: 21 amino acids
Theoretical weight: 2.5 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P05305 (Residues: 53-73; Coverage: 11%)
Gene name: EDN1
Sequence domains: Endothelin family

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.32Å
Relevant EMDB volumes: EMD-38705
Expression systems:
  • Escherichia coli
  • Spodoptera frugiperda
  • Homo sapiens

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