› 8y6i

Electron Microscopy
2.54Å resolution

P-glycoprotein in complex with UIC2 Fab and triple elacridar molecules in nanodisc

Released:
DOI: 10.2210/pdb8y6i/pdb
PDB entry 8y6i coloured by chain, front view.
PDB entry 8y6i coloured by chain, side view.
PDB entry 8y6i coloured by chain, top view.
Source organisms:
Primary publication:
Cryo-EM structure of P-glycoprotein bound to triple elacridar inhibitor molecules.
Hamaguchi-Suzuki N, Adachi N, Moriya T, Yasuda S, Kawasaki M, Suzuki K, Ogasawara S, Anzai N, Senda T, Murata T
Biochem Biophys Res Commun 709 149855 (2024)
PMID: 38579618
Related structures: EMD-38987

Function and Biology Details

Reactions catalysed: 
ATP + H(2)O + phospholipid(Side 1) = ADP + phosphate + phospholipid(Side 2)
ATP + H(2)O + xenobiotic(Side 1) = ADP + phosphate + xenobiotic(Side 2)
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-246336 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
ATP-dependent translocase ABCB1 Chain: A
Molecule details ›
Chain: A
Length: 1534 amino acids
Theoretical weight: 170.54 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P08183 (Residues: 1-1280; Coverage: 100%)
Gene names: ABCB1, MDR1, PGY1
Sequence domains:
UIC2 Fab light chain Chain: B
Molecule details ›
Chain: B
Length: 220 amino acids
Theoretical weight: 24.32 KDa
Source organism: Mus musculus
Expression system: Mus musculus
UIC2 Fab heavy chain Chain: C
Molecule details ›
Chain: C
Length: 225 amino acids
Theoretical weight: 24.38 KDa
Source organism: Mus musculus
Expression system: Mus musculus

Ligands and Environments

3 bound ligands:
Ligand CLR 12 x CLR
Ligand 3PE 1 x 3PE
Ligand R0Z 3 x R0Z
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.54Å
Relevant EMDB volumes: EMD-38987
Expression systems:
  • Homo sapiens
  • Mus musculus

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