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Crystal Structure of RDGC IQ motif/dCaM Complex

X-ray diffraction
2.2Å resolution
PDB entry 8zlw coloured by chain, front view.
PDB entry 8zlw coloured by chain, side view.
PDB entry 8zlw coloured by chain, top view.
Source organisms:
Assembly composition:
hetero trimer (preferred)
Entry contents: 3 distinct polypeptide molecules

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Assemblies

Assembly 1 (preferred)
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Multimeric state: hetero trimer
Accessible surface area: 22540.9 Å2
Buried surface area: 5236.13 Å2
Dissociation area: 968.58 Å2
Dissociation energy (ΔGdiss): -1.88 kcal/mol
Dissociation entropy (TΔSdiss): 12.64 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-292291

Macromolecules

Maltodextrin-binding protein
Chain: A
Length: 376 amino acids
Theoretical weight: 40.81 KDa
Source organism: Escherichia coli #1/H766
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: A0A4P1LXE0 (Residues: 3-371; Coverage: 79%)
Gene names: JW3994, malE
Pfam: Bacterial extracellular solute-binding protein
Maltodextrin-binding protein in PDB entry 8zlw, assembly 1, front view.
Maltodextrin-binding protein in PDB entry 8zlw, assembly 1, side view.
Maltodextrin-binding protein in PDB entry 8zlw, assembly 1, top view.
PDBe-KB: UniProt Coverage View: A0A4P1LXE0  
137650100150200250300350
 
100200300
UniProt
A0A4P1LXE0
Chains
Secondary structure
Interaction interfaces
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Calmodulin
Chain: N
Length: 153 amino acids
Theoretical weight: 17.12 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: P62152 (Residues: 1-149; Coverage: 100%)
Gene names: CG8472, Cam
Pfam: EF-hand domain pair
Calmodulin in PDB entry 8zlw, assembly 1, front view.
Calmodulin in PDB entry 8zlw, assembly 1, side view.
Calmodulin in PDB entry 8zlw, assembly 1, top view.
PDBe-KB: UniProt Coverage View: P62152  
115320406080100120140
 
50100150
UniProt
P62152
Chains
Secondary structure
Ligand binding sites
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Serine/threonine-protein phosphatase rdgC
Chain: R
Length: 33 amino acids
Theoretical weight: 4.27 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: P40421 (Residues: 1-25; Coverage: 4%)
Gene names: CG44746, rdgC
Serine/threonine-protein phosphatase rdgC in PDB entry 8zlw, assembly 1, front view.
Serine/threonine-protein phosphatase rdgC in PDB entry 8zlw, assembly 1, side view.
Serine/threonine-protein phosphatase rdgC in PDB entry 8zlw, assembly 1, top view.
PDBe-KB: UniProt Coverage View: P40421  
13351015202530
 
102030MDENAIRAAIFIQKWYRRHQARREMLEHHHHHH
UniProt
P40421
Chains
Secondary structure
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