› 9asj

Electron Microscopy
3.5Å resolution

Human DNA polymerase theta helicase domain in complex with AMP-PNP, dimer form

Released:
DOI: 10.2210/pdb9asj/pdb
PDB entry 9asj coloured by chain, front view.
PDB entry 9asj coloured by chain, side view.
PDB entry 9asj coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural Basis for Polθ-Helicase DNA Binding and Microhomology-Mediated End-Joining.
Ito F, Li Z, Minakhin L, Khant HA, Pomerantz RT, Chen XS
bioRxiv (2024)
PMID: 38895274
Related structures: EMD-43816

Function and Biology Details

Reactions catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
ATP + H(2)O = ADP + phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-243709 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA polymerase theta Chains: A, B
Molecule details ›
Chains: A, B
Length: 894 amino acids
Theoretical weight: 99.8 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O75417 (Residues: 1-894; Coverage: 35%)
Gene names: POLH, POLQ
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand ANP 2 x ANP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.5Å
Relevant EMDB volumes: EMD-43816
Expression system: Escherichia coli

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