› 9azq

Electron Microscopy
3.82Å resolution

INF2 at the Barbed End of F-Actin with Incoming Actin

Released:
DOI: 10.2210/pdb9azq/pdb
PDB entry 9azq coloured by chain, front view.
PDB entry 9azq coloured by chain, side view.
PDB entry 9azq coloured by chain, top view.
Source organisms:
Primary publication:
Mechanisms of actin filament severing and elongation by formins.
Palmer NJ, Barrie KR, Dominguez R
Nature (2024)
PMID: 38843827
Related structures: EMD-44019

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero nonamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-244828 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Actin, alpha skeletal muscle Chains: A, B, C, D, E, F, I
Molecule details ›
Chains: A, B, C, D, E, F, I
Length: 371 amino acids
Theoretical weight: 41.39 KDa
Source organism: Oryctolagus cuniculus
Expression system: Oryctolagus cuniculus
UniProt:
  • Canonical: P68135 (Residues: 7-377; Coverage: 98%)
Gene names: ACTA, ACTA1
Sequence domains: Actin
Inverted formin-2 Chain: G
Molecule details ›
Chain: G
Length: 60 amino acids
Theoretical weight: 6.87 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q27J81 (Residues: 551-610; Coverage: 5%)
Gene names: C14orf151, C14orf173, INF2
Inverted formin-2 Chain: H
Molecule details ›
Chain: H
Length: 323 amino acids
Theoretical weight: 36.86 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q27J81 (Residues: 625-947; Coverage: 26%)
Gene names: C14orf151, C14orf173, INF2
Sequence domains: Formin Homology 2 Domain

Ligands and Environments

2 bound ligands:
Ligand ADP 7 x ADP
Ligand MG 7 x MG
1 modified residue:
Ligand HIC 7 x HIC

Experiments and Validation Details

Entry percentile scores
Resolution: 3.82Å
Relevant EMDB volumes: EMD-44019
Expression systems:
  • Oryctolagus cuniculus
  • Escherichia coli

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