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INF2 at the Barbed End of F-Actin with Incoming Actin

Electron Microscopy
3.82Å resolution
PDB entry 9azq coloured by chain, front view.
PDB entry 9azq coloured by chain, side view.
PDB entry 9azq coloured by chain, top view.
Source organisms:
Assembly composition:
hetero nonamer (preferred)
Entry contents: 3 distinct polypeptide molecules

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Inverted formin-2 and Actin, alpha skeletal muscle
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Multimeric state: hetero nonamer
Accessible surface area: 117386.28 Å2
Buried surface area: 29676.78 Å2
Dissociation area: 1,232.23 Å2
Dissociation energy (ΔGdiss): -10.12 kcal/mol
Dissociation entropy (TΔSdiss): 15.59 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-244828

Macromolecules

Actin, alpha skeletal muscle
Chains: A, B, C, D, E, F, I
Length: 371 amino acids
Theoretical weight: 41.39 KDa
Source organism: Oryctolagus cuniculus
Expression system: Oryctolagus cuniculus
UniProt:
  • Canonical: P68135 (Residues: 7-377; Coverage: 98%)
Gene names: ACTA, ACTA1
Pfam: Actin
Actin, alpha skeletal muscle in PDB entry 9azq, assembly 1, front view.
Actin, alpha skeletal muscle in PDB entry 9azq, assembly 1, side view.
Actin, alpha skeletal muscle in PDB entry 9azq, assembly 1, top view.
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Inverted formin-2
Chain: G
Length: 60 amino acids
Theoretical weight: 6.87 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q27J81 (Residues: 551-610; Coverage: 5%)
Gene names: C14orf151, C14orf173, INF2
Inverted formin-2 in PDB entry 9azq, assembly 1, front view.
Inverted formin-2 in PDB entry 9azq, assembly 1, side view.
Inverted formin-2 in PDB entry 9azq, assembly 1, top view.
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Inverted formin-2
Chain: H
Length: 323 amino acids
Theoretical weight: 36.86 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q27J81 (Residues: 625-947; Coverage: 26%)
Gene names: C14orf151, C14orf173, INF2
Pfam: Formin Homology 2 Domain
Inverted formin-2 in PDB entry 9azq, assembly 1, front view.
Inverted formin-2 in PDB entry 9azq, assembly 1, side view.
Inverted formin-2 in PDB entry 9azq, assembly 1, top view.
Molecule details ›

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