› › Structure analysis

Ubiquitin E2-Ub-E3 HECT tetrahedral transthiolation intermediate mimic - state 5

Electron Microscopy
3.49Å resolution
PDB entry 9b59 coloured by chain, front view.
PDB entry 9b59 coloured by chain, side view.
PDB entry 9b59 coloured by chain, top view.
Source organism: Schizosaccharomyces pombe 972h-
Assembly composition:
hetero trimer (preferred)
Entry contents: 3 distinct polypeptide molecules

Quick links

Assemblies

Assembly 1 (preferred)
Download    3D Visualisation
Multimeric state: hetero trimer
Accessible surface area: 29209.88 Å2
Buried surface area: 3403.38 Å2
Dissociation area: 511.67 Å2
Dissociation energy (ΔGdiss): -7.54 kcal/mol
Dissociation entropy (TΔSdiss): 11.34 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-272717

Macromolecules

Ubiquitin-conjugating enzyme E2 4
Chain: A
Length: 153 amino acids
Theoretical weight: 17.04 KDa
Source organism: Schizosaccharomyces pombe 972h-
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P46595 (Residues: 1-147; Coverage: 100%)
Gene names: SPBC119.02, ubc4
Pfam: Ubiquitin-conjugating enzyme
Ubiquitin-conjugating enzyme E2 4 in PDB entry 9b59, assembly 1, front view.
Ubiquitin-conjugating enzyme E2 4 in PDB entry 9b59, assembly 1, side view.
Ubiquitin-conjugating enzyme E2 4 in PDB entry 9b59, assembly 1, top view.
Molecule details ›

Search similar proteins

Ubiquitin
Chain: B
Length: 79 amino acids
Theoretical weight: 8.77 KDa
Source organism: Schizosaccharomyces pombe 972h-
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0CH07 (Residues: 1-75; Coverage: 59%)
Gene names: SPAC1805.12c, ubi2, uep1
Pfam: Ubiquitin family
Ubiquitin in PDB entry 9b59, assembly 1, front view.
Ubiquitin in PDB entry 9b59, assembly 1, side view.
Ubiquitin in PDB entry 9b59, assembly 1, top view.
Molecule details ›

Search similar proteins

E3 ubiquitin-protein ligase pub2
Chain: C
Length: 380 amino acids
Theoretical weight: 44.23 KDa
Source organism: Schizosaccharomyces pombe 972h-
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9UTG2 (Residues: 295-671; Coverage: 56%)
Gene names: SPAC1805.15c, pub2
Pfam: HECT-domain (ubiquitin-transferase)
E3 ubiquitin-protein ligase pub2 in PDB entry 9b59, assembly 1, front view.
E3 ubiquitin-protein ligase pub2 in PDB entry 9b59, assembly 1, side view.
E3 ubiquitin-protein ligase pub2 in PDB entry 9b59, assembly 1, top view.
Molecule details ›

Search similar proteins

Quick links