› 9bfh

Electron Microscopy
2.62Å resolution

Cryo-EM co-structure of AcrB with the CU032 efflux pump inhibitor

Released:
DOI: 10.2210/pdb9bfh/pdb
PDB entry 9bfh coloured by chain, front view.
PDB entry 9bfh coloured by chain, side view.
PDB entry 9bfh coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Bacterial efflux pump modulators prevent bacterial growth in macrophages and under broth conditions that mimic the host environment.
Allgood SC, Su C-C, Crooks AL, Meyer CT, Zhou B, Betterton MD, Barbachyn MR, Yu EW, Detweiler CS
OpenAccess logo mBio 14 e0249223 (2023)
PMID: 37921493
Related structures: EMD-44496

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151927 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Multidrug efflux pump subunit AcrB Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 1049 amino acids
Theoretical weight: 113.67 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: P31224 (Residues: 1-1049; Coverage: 100%)
Gene names: JW0451, acrB, acrE, b0462
Sequence domains: AcrB/AcrD/AcrF family

Ligands and Environments

1 bound ligand:
Ligand A1AN8 1 x A1AN8
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.62Å
Relevant EMDB volumes: EMD-44496
Expression system: Escherichia coli K-12

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