› 9bh9

Electron Microscopy
3.5Å resolution

Human DNA polymerase theta helicase domain dimer bound to DNA in the microhomology aligning conformation

Released:
DOI: 10.2210/pdb9bh9/pdb
PDB entry 9bh9 coloured by chain, front view.
PDB entry 9bh9 coloured by chain, side view.
PDB entry 9bh9 coloured by chain, top view.
Source organisms:
Entry authors: Zerio CJ, Lander GC
Related structures: EMD-44537

Function and Biology Details

Reactions catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-271039 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
DNA polymerase theta Chains: A, B
Molecule details ›
Chains: A, B
Length: 893 amino acids
Theoretical weight: 99.67 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O75417 (Residues: 2-894; Coverage: 35%)
Gene names: POLH, POLQ
Sequence domains:
Stem-loop DNA with microhomology in the 3' overhang Chains: X, Y
Molecule details ›
Chains: X, Y
Length: 56 nucleotides
Theoretical weight: 17.26 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.5Å
Relevant EMDB volumes: EMD-44537
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

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