› 9dw8

Electron Microscopy
3.5Å resolution

Dephosphorylated (E1371Q)CFTR in complex with PKA-C

Released:
DOI: 10.2210/pdb9dw8/pdb
PDB entry 9dw8 coloured by chain, front view.
PDB entry 9dw8 coloured by chain, side view.
PDB entry 9dw8 coloured by chain, top view.
Source organisms:
Primary publication:
The structures of protein kinase A in complex with CFTR: Mechanisms of phosphorylation and noncatalytic activation.
Fiedorczuk K, Iordanov I, Mihályi C, Szollosi A, Csanády L, Chen J
Proc Natl Acad Sci U S A 121 e2409049121 (2024)
PMID: 39495916
Related structures: EMD-47238

Function and Biology Details

Reactions catalysed: 
ATP + H(2)O + closed Cl(-) channel = ADP + phosphate + open Cl(-) channel
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-281424 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Cystic fibrosis transmembrane conductance regulator Chain: A
Molecule details ›
Chain: A
Length: 1480 amino acids
Theoretical weight: 168.33 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P13569 (Residues: 1-1480; Coverage: 100%)
Gene names: ABCC7, CFTR
Sequence domains:
cAMP-dependent protein kinase catalytic subunit alpha Chain: G
Molecule details ›
Chain: G
Length: 351 amino acids
Theoretical weight: 40.76 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P00517 (Residues: 1-351; Coverage: 100%)
Gene name: PRKACA
Sequence domains: Protein kinase domain

Ligands and Environments

3 bound ligands:
Ligand B44 2 x B44
Ligand MG 4 x MG
Ligand ANP 1 x ANP
1 modified residue:
Ligand TPO 1 x TPO

Experiments and Validation Details

Entry percentile scores
Resolution: 3.5Å
Relevant EMDB volumes: EMD-47238
Expression system: Homo sapiens

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