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Human pseudouridine synthase 3 (PUS3 D118A mutant) and two pre-tRNA-Arg

Electron Microscopy
2.97Å resolution
PDB entry 9enf coloured by chain, front view.
PDB entry 9enf coloured by chain, side view.
PDB entry 9enf coloured by chain, top view.
Source organism: Homo sapiens
Assembly composition:
hetero tetramer (preferred)
Entry contents: 1 distinct polypeptide molecule
1 distinct RNA molecule

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Assemblies

Assembly 1 (preferred)
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Assembly Name: tRNA pseudouridine(38/39) synthase and RNA
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Multimeric state: hetero tetramer
Accessible surface area: 53163.75 Å2
Buried surface area: 8502.23 Å2
Dissociation area: 2,140.31 Å2
Dissociation energy (ΔGdiss): 13.4 kcal/mol
Dissociation entropy (TΔSdiss): 28.62 kcal/mol
Symmetry number: 2
PDBe Complex ID: PDB-CPX-250383

Macromolecules

tRNA pseudouridine(38/39) synthase
Chains: A, B
Length: 481 amino acids
Theoretical weight: 55.68 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9BZE2 (Residues: 1-481; Coverage: 100%)
Gene names: FKSG32, PUS3
Pfam: tRNA pseudouridine synthase
tRNA pseudouridine(38/39) synthase in PDB entry 9enf, assembly 1, front view.
tRNA pseudouridine(38/39) synthase in PDB entry 9enf, assembly 1, side view.
tRNA pseudouridine(38/39) synthase in PDB entry 9enf, assembly 1, top view.
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RNA chains C, D
Name: pre-tRNA-Arg
Representative chains: C, D
Source organism: Homo sapiens [9606]
Expression system: Not provided
Length: 94 nucleotides
Theoretical weight: 30.3 KDa
Rfam: tRNA
pre-tRNA-Arg in PDB entry 9enf, assembly 1, front view.
pre-tRNA-Arg in PDB entry 9enf, assembly 1, side view.
pre-tRNA-Arg in PDB entry 9enf, assembly 1, top view.
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