Structure analysis

E. coli 70S-TEC complex in delivery state

Electron Microscopy
3.17Å resolution
Source organism: Escherichia coli BL21(DE3)
Assembly composition:
hetero 55-mer (preferred)
Entry contents: 48 distinct polypeptide molecules
7 distinct RNA molecules

Assemblies

Assembly 1 (preferred)
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Multimeric state: hetero 55-mer
PDBe Complex ID: PDB-CPX-495083

Macromolecules

Chain: 0
Length: 55 amino acids
Theoretical weight: 6.39 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A140N5Z7 (Residues: 1-55; Coverage: 100%)
Gene names: ECBD_0090, rpmG
Pfam: Ribosomal protein L33

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Chain: 1
Length: 46 amino acids
Theoretical weight: 5.4 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A140NHV0 (Residues: 1-46; Coverage: 100%)
Gene names: ECBD_4330, rpmH
Pfam: Ribosomal protein L34

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Chain: 2
Length: 65 amino acids
Theoretical weight: 7.31 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A140N9E3 (Residues: 1-65; Coverage: 100%)
Gene names: ECBD_1928, rpmI
Pfam: Ribosomal protein L35

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Chain: 3
Length: 38 amino acids
Theoretical weight: 4.38 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A140N5M6 (Residues: 1-38; Coverage: 100%)
Gene names: ECBD_0452, rpmJ
Pfam: Ribosomal protein L36

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Chain: B
Length: 241 amino acids
Theoretical weight: 26.78 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A140NFK2 (Residues: 1-241; Coverage: 100%)
Gene names: ECBD_3450, rpsB
Pfam: Ribosomal protein S2

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Chain: C
Length: 233 amino acids
Theoretical weight: 26.03 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A140N4K1 (Residues: 1-233; Coverage: 100%)
Gene names: ECBD_0437, rpsC
Pfam:

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Chain: D
Length: 206 amino acids
Theoretical weight: 23.51 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A140N548 (Residues: 1-206; Coverage: 100%)
Gene names: ECBD_0455, rpsD
Pfam:

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Chain: E
Length: 167 amino acids
Theoretical weight: 17.63 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: C3SR27 (Residues: 1-167; Coverage: 100%)
Gene names: A2J79_004072, A5U30_004537, ABE91_009095, ACN81_25445, ACU57_21035, AWP47_12720, B6R15_004084, B6R31_004983, BANRA_01934, BANRA_04313, BB545_28515, BCB93_004278, BG944_001118, BGM66_003608, BGZ_02605, BGZ_04110, BK300_26855, BK383_24720, BKL28_004558, BMT50_12290, BMT91_19995, BRV02_004612, BTB68_004371, BTQ06_10615, BXT93_05550, BvCmsKKP061_03015, BvCmsNSP007_03996, BvCmsSIP010_02670, C0P57_002653, C1Q91_004919, C2M16_25480, C2R31_004618, C3F40_19795, C6669_11440, C719_004902, C7B02_26525, C9160_24325, C9194_22620, C9Z68_22560, CCS08_00140, CF22_004864, CG704_20945, CIG67_10750, CQ842_14400, CQ842_22270, CR538_02495, CTR35_003615, CV83915_02795, CWS33_24865, D3C88_28455, D3G36_23125, D4N09_20180, D9D43_22625, D9E49_05165, D9J61_16660, DD762_23555, DIV22_15250, DL968_17160, DNQ45_03765, DNX30_25470, DS732_24270, DTL43_20990, DU321_12010, E2865_04456, E4K51_21500, E5H86_24515, E6D34_21750, EAI46_07485, ECs4168, EIA08_23965, EIZ93_12460, EN85_004364, EPS76_07205, EPS97_20085, EWK56_25005, ExPECSC038_03655, F7F11_22715, F7N46_24000, F9413_21225, F9461_25645, F9B07_24675, FGAF848_26000, FIJ20_21185, FJQ40_18315, FKO60_25645, FOI11_019250, FOI11_03930, FPI65_20315, FPS11_25420, FVB16_04880, FWK02_06280, FZU14_21830, G3V95_19855, G3W53_20965, G4A38_21520, G4A47_20790, G5603_24595, GAI89_24820, GAJ12_24710, GGB84_004466, GKF66_21555, GNW61_16640, GNZ05_25975, GOP25_22715, GP711_23315, GP944_18800, GP954_00935, GP965_04305, GP975_01195, GP979_01995, GQA06_03940, GQE86_20090, GQM04_10575, GQM13_25210, GQM21_11240, GQN24_22685, GQN34_23275, GRW05_09070, GRW24_04745, GTP92_23185, GUC01_21190, HEP30_018645, HEP34_004769, HHH44_004534, HI055_004141, HIE29_005188, HJQ60_005010, HKA49_005034, HLX92_10065, HLZ39_22205, HLZ50_22170, HMV95_19615, HV109_02180, HV209_14705, HVV39_09275, HVW04_17525, HVW43_18660, HVY77_02165, I6H00_20915, I6H02_12000, IFC14_004401, IH772_24005, J0541_004398, J5U05_004086, J8F57_004248, JNA68_24800, JNP96_25315, NCTC10082_02838, NCTC10089_00501, NCTC10418_00689, NCTC10429_01094, NCTC10764_03862, NCTC10767_01515, NCTC10865_00660, NCTC11126_02770, NCTC11181_02737, NCTC11341_01890, NCTC7922_04043, NCTC7927_00550, NCTC8179_05941, NCTC8333_00488, NCTC8621_00507, NCTC8622_00508, NCTC8959_03262, NCTC8960_03069, NCTC9001_04253, NCTC9045_00565, NCTC9075_00723, NCTC9077_00603, NCTC9702_00536, NCTC9706_02736, NCTC9962_06796, NOI85_25610, NY836_17895, OFN31_22790, OGM49_22340, P6223_003984, PWL68_004850, PWL68_005335, Q2V20_09695, Q2V64_25390, QDW62_02185, QO046_13765, R8G00_13250, R8O40_004922, RZR61_12900, SAMEA3472044_01161, SAMEA3472056_05412, SAMEA3752557_01953, V9Z47_02405, WR15_19615, rpsE
Pfam:

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Chain: F
Length: 135 amino acids
Theoretical weight: 15.73 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P02358 (Residues: 1-135; Coverage: 100%)
Gene names: JW4158, b4200, rpsF
Pfam: Ribosomal protein S6

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Chain: G
Length: 179 amino acids
Theoretical weight: 20.06 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: C4ZUJ6 (Residues: 1-179; Coverage: 100%)
Gene names: BWG_3032, rpsG
Pfam: Ribosomal protein S7p/S5e

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Chain: H
Length: 130 amino acids
Theoretical weight: 14.15 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A140N537 (Residues: 1-130; Coverage: 100%)
Gene names: ECBD_0445, rpsH
Pfam: Ribosomal protein S8

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Chain: I
Length: 130 amino acids
Theoretical weight: 14.89 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A140N2Z9 (Residues: 1-130; Coverage: 100%)
Gene names: ECBD_0517, rpsI
Pfam: Ribosomal protein S9/S16

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Chain: J
Length: 103 amino acids
Theoretical weight: 11.76 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A140N6Y5 (Residues: 1-103; Coverage: 100%)
Gene names: ECBD_0430, rpsJ
Pfam: Ribosomal protein S10p/S20e

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Chain: K
Length: 129 amino acids
Theoretical weight: 13.87 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A140N7L9 (Residues: 1-129; Coverage: 100%)
Gene names: ECBD_0454, rpsK
Pfam: Ribosomal protein S11

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Chain: L
Length: 124 amino acids
Theoretical weight: 13.81 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A140N4H3 (Residues: 1-124; Coverage: 100%)
Gene names: ECBD_0407, rpsL
Pfam: Ribosomal protein S12/S23

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Chain: M
Length: 118 amino acids
Theoretical weight: 13.13 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A140N5B4 (Residues: 1-118; Coverage: 100%)
Gene names: ECBD_0453, rpsM
Pfam: Ribosomal protein S13/S18

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Chain: N
Length: 101 amino acids
Theoretical weight: 11.61 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A140N7K8 (Residues: 1-101; Coverage: 100%)
Gene names: ECBD_0444, rpsN
Pfam: Ribosomal protein S14p/S29e

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Chain: O
Length: 89 amino acids
Theoretical weight: 10.29 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A140N811 (Residues: 1-89; Coverage: 100%)
Gene names: ECBD_0575, rpsO
Pfam: Ribosomal protein S15

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Chain: P
Length: 82 amino acids
Theoretical weight: 9.21 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A140N7D8 (Residues: 1-82; Coverage: 100%)
Gene names: ECBD_1077, rpsP
Pfam: Ribosomal protein S16

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Chain: Q
Length: 84 amino acids
Theoretical weight: 9.72 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A140N6Z6 (Residues: 1-84; Coverage: 100%)
Gene names: ECBD_0440, rpsQ
Pfam: Ribosomal protein S17

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Chain: R
Length: 75 amino acids
Theoretical weight: 9.01 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0AAD1DH49 (Residues: 1-75; Coverage: 100%)
Gene names: EGY08_12410, rpsR

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Chain: S
Length: 92 amino acids
Theoretical weight: 10.46 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A140N528 (Residues: 1-92; Coverage: 100%)
Gene names: ECBD_0435, rpsS
Pfam: Ribosomal protein S19

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Chain: T
Length: 87 amino acids
Theoretical weight: 9.71 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A140NFU3 (Residues: 1-87; Coverage: 100%)
Gene names: ECBD_3593, rpsT
Pfam: Ribosomal protein S20

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Chain: U
Length: 71 amino acids
Theoretical weight: 8.52 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A6I7DAD8 (Residues: 1-71; Coverage: 100%)
Gene names: F1325_15275, rpsU
Pfam: Ribosomal protein S21

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Chain: c
Length: 273 amino acids
Theoretical weight: 29.92 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A140N7J1 (Residues: 1-273; Coverage: 100%)
Gene names: ECBD_0434, rplB
Pfam:

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Chain: d
Length: 209 amino acids
Theoretical weight: 22.29 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A140N3G7 (Residues: 1-209; Coverage: 100%)
Gene names: ECBD_0431, rplC
Pfam: Ribosomal protein L3

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Chain: e
Length: 201 amino acids
Theoretical weight: 22.12 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A140N5K8 (Residues: 1-201; Coverage: 100%)
Gene names: ECBD_0432, rplD
Pfam: Ribosomal protein L4/L1 family

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Chain: f
Length: 179 amino acids
Theoretical weight: 20.33 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A140N5A3 (Residues: 1-179; Coverage: 100%)
Gene names: ECBD_0443, rplE
Pfam:

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Chain: g
Length: 177 amino acids
Theoretical weight: 18.93 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A140N2T1 (Residues: 1-177; Coverage: 100%)
Gene names: ECBD_0446, rplF
Pfam: Ribosomal protein L6

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Chain: h
Length: 149 amino acids
Theoretical weight: 15.79 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A140NDV1 (Residues: 1-149; Coverage: 100%)
Gene names: ECBD_3831, rplI
Pfam:

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Chain: i
Length: 142 amino acids
Theoretical weight: 16.05 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A140N598 (Residues: 1-142; Coverage: 100%)
Gene names: ECBD_0516, rplM
Pfam: Ribosomal protein L13

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Chain: j
Length: 123 amino acids
Theoretical weight: 13.57 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A140N3H4 (Residues: 1-123; Coverage: 100%)
Gene names: ECBD_0441, rplN
Pfam: Ribosomal protein L14p/L23e

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Chain: k
Length: 144 amino acids
Theoretical weight: 15.01 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A140N711 (Residues: 1-144; Coverage: 100%)
Gene names: ECBD_0450, rplO
Pfam: Ribosomal proteins 50S-L15, 50S-L18e, 60S-L27A

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Chain: l
Length: 136 amino acids
Theoretical weight: 15.33 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A140N6Z2 (Residues: 1-136; Coverage: 100%)
Gene names: ECBD_0438, rplP
Pfam: Ribosomal protein L16p/L10e

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Chain: m
Length: 127 amino acids
Theoretical weight: 14.39 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A140N4M0 (Residues: 1-127; Coverage: 100%)
Gene names: ECBD_0457, rplQ
Pfam: Ribosomal protein L17

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Chain: n
Length: 117 amino acids
Theoretical weight: 12.79 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A140N4L0 (Residues: 1-117; Coverage: 100%)
Gene names: ECBD_0447, rplR
Pfam: Ribosomal L18 of archaea, bacteria, mitoch. and chloroplast

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Chain: o
Length: 115 amino acids
Theoretical weight: 13.16 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A140N6T7 (Residues: 1-115; Coverage: 100%)
Gene names: ECBD_1080, rplS
Pfam: Ribosomal protein L19

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Chain: p
Length: 118 amino acids
Theoretical weight: 13.53 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A140NBS1 (Residues: 1-118; Coverage: 100%)
Gene names: ECBD_1929, rplT
Pfam: Ribosomal protein L20

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Chain: q
Length: 103 amino acids
Theoretical weight: 11.59 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A140N5D7 (Residues: 1-103; Coverage: 100%)
Gene names: ECBD_0556, rplU
Pfam: Ribosomal prokaryotic L21 protein

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Chain: r
Length: 110 amino acids
Theoretical weight: 12.25 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A140N2S3 (Residues: 1-110; Coverage: 100%)
Gene names: ECBD_0436, rplV
Pfam: Ribosomal protein L22p/L17e

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Chain: s
Length: 100 amino acids
Theoretical weight: 11.22 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: C6EGE8 (Residues: 1-100; Coverage: 100%)
Gene names: ECBD_0433, rplW
Pfam: Ribosomal protein L23

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Chain: t
Length: 104 amino acids
Theoretical weight: 11.34 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A140N5L7 (Residues: 1-104; Coverage: 100%)
Gene names: ECBD_0442, rplX
Pfam:

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Chain: u
Length: 94 amino acids
Theoretical weight: 10.71 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A140N846 (Residues: 1-94; Coverage: 100%)
Gene names: ECBD_1472, rplY
Pfam: Ribosomal L25p family

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Chain: v
Length: 85 amino acids
Theoretical weight: 9.15 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A140N340 (Residues: 1-85; Coverage: 100%)
Gene names: ECBD_0557, rpmA
Pfam: Ribosomal L27 protein

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Chain: w
Length: 78 amino acids
Theoretical weight: 9.03 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A140N3L9 (Residues: 1-78; Coverage: 100%)
Gene names: ECBD_0089, rpmB
Pfam: Ribosomal L28 family

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Chain: x
Length: 63 amino acids
Theoretical weight: 7.29 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A140N7F5 (Residues: 1-63; Coverage: 100%)
Gene names: ECBD_0439, rpmC
Pfam: Ribosomal L29 protein

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Chain: y
Length: 59 amino acids
Theoretical weight: 6.55 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A140N7G4 (Residues: 1-59; Coverage: 100%)
Gene names: ECBD_0449, rpmD
Pfam: Ribosomal protein L30p/L7e

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Chain: z
Length: 57 amino acids
Theoretical weight: 6.46 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A140NAZ2 (Residues: 1-57; Coverage: 100%)
Gene names: ECBD_2512, rpmF
Pfam: Ribosomal L32p protein family

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Name: 16S ribosomal RNA
Representative chains: A
Length: 1542 nucleotides
Theoretical weight: 499.86 KDa

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Name: mRNA in ribosome channel
Representative chains: X
Length: 16 nucleotides
Theoretical weight: 5 KDa

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Name: Phe-NH-tRNA(Phe) A-site
Representative chains: Z
Length: 76 nucleotides
Theoretical weight: 24.75 KDa

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Name: 23S ribosomal RNA
Representative chains: a
Length: 2925 nucleotides
Theoretical weight: 948.63 KDa

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Name: 5S ribosomal RNA
Representative chains: b
Length: 119 nucleotides
Theoretical weight: 38.48 KDa

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Name: mRNA in SD-aSD duplex
Representative chains: 4
Length: 10 nucleotides
Theoretical weight: 3.3 KDa

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Name: tRNA(fmet) P-site
Representative chains: Y
Length: 77 nucleotides
Theoretical weight: 24.83 KDa

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