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Chart Builder documentation
Summary
Introduction
The EMDB Chart Builder (https://emdb-empiar.org/statistics/builder) is a web-based application that provides users with the ability to create customizable, dynamic, and informative charts that help analyse the holdings and trends of the EMDB and EMPIAR archives, powered by the EMDB search engine. The underlying data is based on the metadata of all EMDB entries, enriched by the EMICSS resource (https://emdb-empiar.org/emicss). This tool allows users to define specific search terms, enabling the extraction of data subsets that are directly relevant to their analysis objectives. By organising the extracted data into meaningful categories, users can generate insightful charts to visualise and analyse the results. The Chart Builder can be accessed at https://ebi.ac.uk/emdb/statistics/builder.This page presents a user-friendly interface specifically designed to facilitate the creation of customised charts using both EMDB and EMPIAR data.
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Multiple data series definition -
Plot charts from categorical variables -
Multiple stack modes for the charts. -
Display the data in linear or logarithmic scales.
Use of the Chart Builder requires some knowledge of the EMDB search engine. Therefore you might find it useful to read the search system documentation and the list of search fields. These resources provide valuable insights into the various search functionalities and parameters that can be employed to refine your data extraction.
The Chart builder now allows you to open and customise most of the charts available at /emdb/statistics. If you see an edit icon, simply click on it to open the corresponding chart in the Chart builder. This feature provides you with the flexibility to modify and personalise the chart according to your specific requirements, enabling you to derive deeper insights from the data visualisation.
Fields
Archive
Select if your chart is going to be using data about EMDB or EMPIAR. (Note: the amount of available metadata is currently much greater for EMDB than for EMPIAR.)
Chart type
The Chart Builder currently supports four types of charts:
- Line: This chart type is best suited for visualising trends and changes over time. It can be used to
illustrate how properties of one or more subsets of entries evolve over time.Column: The column chart
is ideal for comparing different categories or groups.
Figure 2: Line chart example. - Bar: The bar chart is ideal for comparing different categories or groups. It provides a visual
representation of the distribution and relative magnitudes of specific entries within each category.
Figure 3: Bar chart example. - Pie: The pie chart is a useful tool for illustrating proportions and percentages. It provides a concise
visual representation of the distribution of a selected subset of entries among various categories.
Pie charts can only have one data series, and the data categories are defined by the Data X parameter.
Figure 4: Pie chart example. - Area: Similar to a line chart, the area chart emphasises the cumulative size of subsets of entries over time.
Figure 5: Area chart example.
Global filter
The optional global filter in the Chart Builder allows you to specify a search term that will be applied to the selected entries for the entire chart. This feature ensures that the resulting plot focuses on the specific data subset defined by the search term. For instance, suppose you wish to create a plot showcasing the number of entries related to Sars-Cov-2 per EM method. In that case, you can simply change one of the previous examples and set the following query within the global filter: `natural_source_ncbi_code:"2697049"`. This query narrows down the data to entries associated with the Sars-Cov-2 NCBI code, enabling the creation of a customised plot that visualises the desired information accurately.
Data X
The Data X field determines the data that will be displayed on the X-axis or as categories in a pie chart. Within this field, there are three types of data X definitions:
Year:
The X-axis values in the chart correspond to the release year of the entries. By modifying the Data X settings, you can refine the previously created SARS-CoV-2 chart to display data specifically from the year 2019 and beyond.
Experimental metadata:
When selecting the option of "Experimental metadata" for the Data X field, you have the ability to choose one of the enumeration categories. The chosen category's elements will be displayed on the X-axis of the chart. For instance, you can set the experimental metadata category as an EM method to showcase the number of Sars-Cov-2 entries per each type of experiment.
Custom queries:
You can define custom queries to specify a set of criteria that will be displayed on the x-axis of the chart. This allows you to customise the chart further according to your specific requirements. For example, you can modify the previous chart to include only the columns related to single-particle, subtomogram averaging, and tomography by using custom queries.
Custom queries are particularly important when creating pie charts since they can only have a single data series, and the slices of the pie are defined by the elements in the custom queries. We can modify the previous chart to be displayed as a pie chart. This allows for a concise visual representation of the distribution of the selected subset of entries among each experiment method.
Data Y:
The Data Y field is used to set what information is going to be displayed along the Y-axis of your chart. For example, you can choose to display the number of entries, publications, or resolution on the Y-axis, depending on the nature of your analysis and the insights you seek to derive from the chart.
Data series:
This field defines the data series that will be displayed in your chart. A data series consists of three pieces of information: operator, query, and label. The available operators depend on the selection made in the Data Y field. If Data Y is a numeric variable, the operator can be average, minimum, maximum, or sum. On the other hand, if Data Y is a categorical variable, the operator can be the count of unique values or cumulative values. Additionally, you can apply an optional filter query for each data series. This is useful when comparing multiple classes of information, as demonstrated in the example below.
Attributes
There are seven attributes that can be used to print, download, export and change the chart visualisation:
- Change how area and columns are displayed. There are three options: unstacked, stacked and stacked percent.
- Select to display the data series in a logarithmic or linear scale.
- Share the current chart. The resulting URL will be copied into the clipboard.
- Print the current chart.
- Download the chart image or data table.
- Full Screen mode.
- Extra options.
Quick links
Recent Entries
(Show all)In situ human unrotated hibernating with CCDC124 state 80S ribosome
In situ human Hibernating class1 (rotate3) without E tRNA state 80S ribosome
In situ human Hibernating rotate3 with Z site tRNA state 80S ribosome
In situ human Hibernating rotate 3 with E-site tRNA state 80S ribosome
C. thermocellum UvrA-UvrB in complex with DNA with a fluorescein modification and AMPPNP: consensus map
C. thermocellum UvrA-UvrB in complex with DNA with a fluorescein modification and AMPPNP: focused map
Structure of the Chaetomium thermophilum Pmt4 homodimer (C2 symmetry)
Structure of the Chaetomium thermophilum Pmt4 homodimer (C1 symmetry)
Structure of human 1918 influenza A polymerase heterotrimer in complex with 1918 NEP.
Yeast 80S with nascent chain in complex with Ssb1-ADP in the S1 state
Structure of human 1918 influenza A polymerase heterotrimer in complex with WSN NEP.
structure of bundle-shaped PBS with both long rod and (ApcA2B3ApcD) trimer
Structure of the energy converting methyltransferase (Mtr) of Methanosarcina mazei in complex with a novel protein binder
Structure of the energy converting methyltransferase (Mtr) of Methanosarcina mazei in complex with a novel protein binder
Structure of the energy converting methyltransferase (Mtr) of Methanosarcina mazei in complex with a novel protein binder
Structure of the energy converting methyltransferase (Mtr) of Methanosarcina mazei in complex with a novel protein binder
Subtomogram averaging of HTLV-1 Gag capsid from immature particles
Microtubules in presence of Ndc80 and Ska, non-Ndc80 microtubule-bound molecules
Focused-Refinement Cryo-EM Map of the RAZR-ZFD Domain within the In Vitro Reconstituted RAZR.Gp77 Complex
Cryo-EM Structure of Gp77 within the In Vitro Reconstituted RAZR:GP77 Complex
Cryo-EM Map of the Copurified Ring-Activated Zinc-Finger RNase (RAZR, H154A) in Complex with Phage Protein Gp77
Cryo-EM map of the in vitro reconstituted RAZR:GP77 complex with AlphaFold-predicted models fitted into the density.
Cryo-EM structure of the dCas12f-gRNA-DNA complex (partial R-Loop)
Pol II-DSIF-SPT6-PAF1c-TFIIS-IWS1-ELOF1-LEDGF-nucleosome CDC73 local map J
Cryo-EM structure of active human green cone opsin in complex with chimeric G protein (miniGist)
Pol II-DSIF-SPT6-PAF1c-TFIIS-IWS1-ELOF1-LEDGF-nucleosome RPB5 local map I
Pol II-DSIF-SPT6-PAF1c-TFIIS-IWS1-ELOF1-LEDGF-nucleosome non-template DNA local map H
Pol II-DSIF-SPT6-PAF1c-TFIIS-IWS1-ELOF1-LEDGF-nucleosome CTR9+WDR61 local map K
Pol II-DSIF-SPT6-PAF1c-TFIIS-IWS1-ELOF1-LEDGF-nucleosome CTR9 TPR local map L
Pol II-DSIF-SPT6-PAF1c-TFIIS-IWS1-ELOF1-LEDGF-nucleosome PAF1+LEO1 local map M
Pol II-DSIF-SPT6-PAF1c-TFIIS-IWS1-ELOF1-LEDGF-nucleosome LEO1 C-terminus map N
Pol II-DSIF-SPT6-PAF1c-TFIIS-IWS1-ELOF1-LEDGF-nucleosome SPT6 local map O
Subtomogram average of influenza hemagglutinin (A/Puerto Rico/8/1934)
Mycobacterium smegmatis 70S ribosome with small molecule drug MK-7762
Constricted-pore class from combined 0, 10, 20, 30 mM ATP datasets
Cryo-EM structure of SARS-CoV-2 KP.2 spike RBD in complex with ACE2
Yeast 80S with nascent chain in complex with Ssb1-ADP in the S2 state
Human pannexin 1 channel from combined 0, 10, 20, 30 mM ATP datasets
Cryo-EM reconstruction of PI3KC3-C2 in complex with Rubicon Middle Region of C terminus, focused map 2/2
Cryo-EM reconstruction of PI3KC3-C2 in complex with Rubicon Middle Region of C terminus, composite map
Cryo-EM reconstruction of PI3KC3-C2 in complex with Rubicon Middle Region of C terminus, focused map 1/2
Cryo-EM structure of human PI3KC3-C2 in complex with Rubicon Middle Region of C terminus
Co-transcriptional histone H3K36 methylation complex containing RNA polymerase II elongation complex, Set2, and the upstream nucleosome. (temp115, type A)
Co-transcriptional histone H3K36 methylation complex containing RNA polymerase II elongation complex, Set2, and the upstream nucleosome. (temp115, type B)
Co-transcriptional histone H3K36 methylation complex containing RNA polymerase II elongation complex, Set2, and the upstream nucleosome. (temp130, type B)
Co-transcriptional histone H3K36 methylation complex containing RNA polymerase II elongation complex, Set2, and the upstream nucleosome. (temp130, type A)
The complex structure of 0086-0043 and NET determined with Cryo-EM.
The complex structure of Y510-9709 and NET determined with Cryo-EM
Co-transcriptional histone H3K36 methylation complex containing RNA polymerase II elongation complex, Set2, and the upstream nucleosome. (temp115, FACT-hexamer)
consensus map of Neddylated CUL5-ARIH2-L3A2-1 bound to ASB9-EloB/C-CKB
Structure of the Complement classical and lectin pathway C3 convertase
Consensus Map of Neddylated CUL5 C-terminal region-RBX2-ARIH2~L3A2-1~Ub
Structure of the Complement classical and lectin pathway proconvertase, C4b2
Structure of the Complement classical and lectin pathway C3 convertase in complex with substrate C3
Focus refined map of Neddylated CUL5 C-terminal region-RBX2-ARIH2~L3A2-1~Ub
Cerebellar GluA2/4 NTD heterophilic tetramer interface (focused refinement)
GluA4 N-terminal domain bound to nanobody NB74 (focused refinement)
Structure of Neddylated CUL5 C-terminal region-RBX2-ARIH2~L3A2-1~Ub
Structure of the Complement classical and lectin pathway proconvertase, C4b2
Focus refined map of Neddylated CUL5-ARIH2-L3A2-1 bound to ASB9-EloB/C-CKB, focus refined on ARIH2-L3A2-1
Cryo-EM map of focus refined ASB9-Elob/C-CKB bound to Nedd8-CUL5-RBX2-ARIH2-L3A2-1
P116 from Mycoplasma pneumoniae in complex with mild growth suppressor monoclonal antibody
cryoEM structure of the B-chain of the human OGA-L Catalytic Dimer
cryoEM structure of the A-chain of the human OGA-L Catalytic Dimer
cryoEM structure of the human OGA-L Catalytic Dimer, extra A-chain density
Low-resolution electron density map of C. elegans PEZO-1 Isoform L
Structure-Guided Design of Picomolar-level Macrocyclic TRPC5 Channel Inhibitors with Antidepressant Activity
Structure of the wild-type ABCC2 in Arabidopsis thaliana in the apo state
Cryo-EM structure of amyloid peptide-silk block protein fibril, Type 2
Structure of ABCC2(E1404Q) in Arabidopsis thaliana in the apo state
Structure of ABCC2(E1404Q) in Arabidopsis thaliana in the DNP-GS bound state
Structure of ABCC2(E1404Q) monomer in Arabidopsis thaliana in the DNP-GS bound state
Structure of ABCC2(E1404Q) in Arabidopsis thaliana in the ATP bound state
Structure of the wild-type ABCC2 dimer in Arabidopsis thaliana in the apo state
Cryo-EM structure of renal amyloid fibril from an immunoglobulin light chain amyloidosis patient in polymorph B
Cryo-EM structure of renal amyloid fibril from an immunoglobulin light chain amyloidosis patient in polymorph A
Consensus map of KATP channel in complex with centipede toxin SpTx1
C. thermocellum UvrA in complex with unmodified DNA and AMPPNP (ATP-bound conformation 1)
C. thermocellum UvrA in complex with unmodified DNA and AMPPNP (ATP-bound conformation 2)
C. thermocellum UvrA in complex with unmodified DNA and AMPPNP (basal conformation)
C. thermocellum UvrA in complex with DNA with a fluorescein modification (basal conformation)
C. thermocellum UvrA in complex with DNA with a fluorescein modification and AMPPNP (ATP-bound conformation 1)
C. thermocellum UvrA in complex with DNA with an abasic site (basal conformation)
C. thermocellum UvrA in complex with DNA with an abasic site and AMPPNP (ATP-bound conformation 2)
C. thermocellum UvrA in complex with DNA with an abasic site and AMPPNP (ATP-bound conformation 1)
C. thermocellum UvrA in complex with DNA with a fluorescein modification and ADP (basal conformation)
C. thermocellum UvrA (K647A) in complex with DNA with a fluorescein modification (basal conformation)
C. thermocellum UvrA (K39A) in complex with DNA with a fluorescein modification and AMPPNP (basal conformation)
C. thermocellum UvrA-UvrB in complex with DNA with a fluorescein modification and AMPPNP (composite map)
a5b3 GABAAR bound to Etomidate, GABA, and Mb25 in a desensitized state in saposin nanodiscs
a5b3 GABAA Receptor bound to GABA and Mb25 in desensitized state in detergent micelles
a5b3 GABAA Receptor in 1 a5 to 4 b3 stoichiometry in desensitized state
a5b3 GABAAR bound to GABA and Mb25 in a desensitized state in saposin nanodiscs after long GABA treatment
Hexamer Msp1 from S.cerevisiae (with a catalytic dead mutation) in complex with an unknown peptide substrate
Core filament of the spirochete periplasmic flagella of Leptospira biflexa
core filament of the spirochete periplasmic flagella of Leptospira biflexa wild type
core filament of the spirochete periplasmic flagella of Leptospira biflexa deleted fcpB strain
Octamer Msp1 from S.cerevisiae(with a catalytic dead mutation) in complex with an unknown peptide substrate
core filament of the spirochete periplasmic flagella of Leptospira biflexa from the flaA2-complemented stain
Straight and symmetrical filament of the spirochete periplasmic flagella of Leptospira biflexa
core filament of the spirochete periplasmic flagella of Leptospira biflexa from the deleted fcpB_CL13 strain
Sheathed filament of the spirochete periplasmic flagella of Leptospira biflexa from the deleted fcpB_CL13 strain
Straight and symmetrical filament of the spirochete periplasmic flagella of Leptospira biflexa deleted fcpB strain
Heptamer Msp1 from S.cerevisiae(with a catalytic dead mutation) in complex with an unknown peptide substrate
sheathed filament of the spirochete periplasmic flagella of Leptospira biflexa wild type
sheathed filament of the spirochete periplasmic flagella of Leptospira biflexa from the flaA2-complemented stain
Negative stain EM map of EBV glycoprotein gH/gL in complex with glycoprotein gp42 and FAB ATX-42-1.1 partially open conformation
Negative stain EM map of EBV glycoprotein gH/gL in complex with glycoprotein gp42 and FAB ATX-42-2 closed conformation
Negative stain EM map of EBV glycoprotein gH/gL in complex with glycoprotein gp42 and HLA-DR1 Beta chain
Negative Stain EM map of EBV glycoprotein gp350 in complex with ATX-350-2 FAB
Negative Stain EM map of EBV glycoprotein gp350 in complex with 72A1 FAB
Negative Stain EM map of EBV glycoprotein gp350 in complex with ATX-350-1 FAB
Negative Stain EM map of EBV glycoprotein gp350 in complex with ATX-350-1 FAB and 72A1 FAB
Structure of a LGR dimer from Caenorhabditis elegans in apo state
Negative stain EM map of EBV glycoprotein gH/gL in complex with glycoprotein gp42 and FAB ATX-42-2 Open conformation
Globular domain of monkeypox virus OPG153 (A28) in complex with antibodies 08E11 and 12I12
Globular domain of monkeypox virus OPG153 (A28) bound to antibody 02M12
Negative stain EM map of EBV glycoprotein gH/gL in complex with glycoprotein gp42 and FAB ATX-42-1.1 open conformation
Cryo-EM structure of MERS-CoV nsp10-nsp14 (E191A) in complex with T20P14-G RNA
Cryo-EM structure of MERS-CoV nsp10-nsp14 (E191A) in complex with T20P14-A RNA
Cryo-EM structure of MERS-CoV nsp10-nsp14 (E191A) in complex with T20P14-U RNA
Cryo-EM structure of MERS-CoV nsp10-nsp14 (E191A) in complex with T20P15 RNA, dimeric form
Cryo-EM structure of MERS-CoV nsp10-nsp14 (E191A) in complex with T20P15 RNA, monomeric form
In situ HHT and CHX treated human eEF1A-A/T-P-Z state 80S ribosome
Structure of E. Coli DNA protection during starvation protein (DPS) from single particle cryoEM
In situ human unrotated hibernating without CCDC124 state 80S ribosome
