Cryo-EM imaging scaffold subunits A and B used to display KRAS G12C complex with GDP
Resolution: 2.2 Å
EM Method: Single-particle
Fitted PDBs: 8g3k
Q-score: 0.668
Castells-Graells R, Meador K, Arbing MA, Sawaya MR, Gee M, Cascio D, Gleave E, Debreczeni JE, Breed J, Leopold K, Patel A, Jahagirdar D, Lyons B, Subramaniam S, Phillips C, Yeates TO
PNAS (2023) 120 pp. e2305494120-e2305494120 [ DOI: doi:10.1073/pnas.2305494120 Pubmed: 37669364 ]
KRAS G12C complex with GDP imaged on a cryo-EM imaging scaffold
Resolution: 3.02 Å
EM Method: Single-particle
Fitted PDBs: 8g42
Q-score: 0.504
Castells-Graells R, Meador K, Arbing MA, Sawaya MR, Gee M, Cascio D, Gleave E, Debreczeni JE, Breed J, Leopold K, Patel A, Jahagirdar D, Lyons B, Subramaniam S, Phillips C, Yeates TO
PNAS (2023) 120 pp. e2305494120-e2305494120 [ Pubmed: 37669364 DOI: doi:10.1073/pnas.2305494120 ]
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Rcg-33 - cryo-em imaging scaffold subunit b fused to darpin (35 kDa, Protein from synthetic construct)
- Gtpase kras (21 kDa, Protein from Homo sapiens)
- Kras g12c displayed on a cryo-em imaging scaffold (Complex from synthetic construct)
KRAS G12C complex with GDP and AMG 510 imaged on a cryo-EM imaging scaffold
Resolution: 3.19 Å
EM Method: Single-particle
Fitted PDBs: 8g47
Q-score: 0.465
Castells-Graells R, Meador K, Arbing MA, Sawaya MR, Gee M, Cascio D, Gleave E, Debreczeni JE, Breed J, Leopold K, Patel A, Jahagirdar D, Lyons B, Subramaniam S, Phillips C, Yeates TO
PNAS (2023) 120 pp. e2305494120-e2305494120 [ Pubmed: 37669364 DOI: doi:10.1073/pnas.2305494120 ]
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Rcg-33 - cryo-em imaging scaffold subunit b fused to darpin (35 kDa, Protein from synthetic construct)
- Amg 510 (bound form) (562 Da, Ligand)
- Kras g12c complex with gdp and amg 510 imaged on a cryo-em imaging scaffold (Complex from synthetic construct)
- Gtpase kras (21 kDa, Protein from Homo sapiens)
Green Fluorescence Protein imaged on a cryo-EM imaging scaffold
Resolution: 2.98 Å
EM Method: Single-particle
Fitted PDBs: 8g4e
Q-score: 0.566
Castells-Graells R, Meador K, Arbing MA, Sawaya MR, Gee M, Cascio D, Gleave E, Debreczeni JE, Breed J, Leopold K, Patel A, Jahagirdar D, Lyons B, Subramaniam S, Phillips C, Yeates TO
PNAS (2023) 120 pp. e2305494120-e2305494120 [ DOI: doi:10.1073/pnas.2305494120 Pubmed: 37669364 ]
KRAS G12V complex with GDP imaged on a cryo-EM imaging scaffold
Resolution: 2.91 Å
EM Method: Single-particle
Fitted PDBs: 8g4f
Q-score: 0.546
Castells-Graells R, Meador K, Arbing MA, Sawaya MR, Gee M, Cascio D, Gleave E, Debreczeni JE, Breed J, Leopold K, Patel A, Jahagirdar D, Lyons B, Subramaniam S, Phillips C, Yeates TO
PNAS (2023) 120 pp. e2305494120-e2305494120 [ DOI: doi:10.1073/pnas.2305494120 Pubmed: 37669364 ]
- Rcg-33 - cryo-em imaging scaffold subunit b fused to darpin (35 kDa, Protein from synthetic construct)
- Kras g12v complex with gdp displayed on a cryo-em imaging scaffold (Complex from synthetic construct)
- Water (18 Da, Ligand)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Gtpase kras (19 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
KRAS G13C complex with GDP imaged on a cryo-EM imaging scaffold
Resolution: 2.87 Å
EM Method: Single-particle
Fitted PDBs: 8g4h
Q-score: 0.548
Castells-Graells R, Meador K, Arbing MA, Sawaya MR, Gee M, Cascio D, Gleave E, Debreczeni JE, Breed J, Leopold K, Patel A, Jahagirdar D, Lyons B, Subramaniam S, Phillips C, Yeates TO
PNAS (2023) 120 pp. e2305494120-e2305494120 [ DOI: doi:10.1073/pnas.2305494120 Pubmed: 37669364 ]
- Kras g13c complex with gdp displayed on a cryo-em imaging scaffold (Complex from synthetic construct)
- Gtpase kras (19 kDa, Protein from Homo sapiens)
- Rcg-33 - cryo-em imaging scaffold subunit b fused to darpin (35 kDa, Protein from synthetic construct)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
Cryo-EM structure of the SARS-CoV-2 N501Y mutant spike protein ectodomain
Resolution: 2.81 Å
EM Method: Single-particle
Fitted PDBs: 7mjg
Q-score: 0.421
Zhu X, Mannar D, Srivastava SS, Berezuk AM, Demers JP, Saville JW, Leopold K, Li W, Dimitrov DS, Tuttle KS, Zhou S, Chittori S, Subramaniam S
PLoS Biol (2021) 19 pp. e3001237-e3001237 [ Pubmed: 33914735 DOI: doi:10.1371/journal.pbio.3001237 ]
Cryo-EM structure of the SARS-CoV-2 N501Y mutant spike protein ectodomain bound to VH ab8
Resolution: 2.66 Å
EM Method: Single-particle
Fitted PDBs: 7mjh
Q-score: 0.394
Zhu X, Mannar D, Srivastava SS, Berezuk AM, Demers JP, Saville JW, Leopold K, Li W, Dimitrov DS, Tuttle KS, Zhou S, Chittori S, Subramaniam S
PLoS Biol (2021) 19 pp. e3001237-e3001237 [ Pubmed: 33914735 DOI: doi:10.1371/journal.pbio.3001237 ]
- Vh ab8 (15 kDa, Protein from synthetic construct)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Spike glycoprotein (142 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Sars-cov-2 n501y mutant spike protein ectodomain bound to vh ab8 (Complex from Severe acute respiratory syndrome coronavirus 2)
Resolution: 2.81 Å
EM Method: Single-particle
Fitted PDBs: 7mji
Q-score: 0.516
Zhu X, Mannar D, Srivastava SS, Berezuk AM, Demers JP, Saville JW, Leopold K, Li W, Dimitrov DS, Tuttle KS, Zhou S, Chittori S, Subramaniam S
PLoS Biol (2021) 19 pp. e3001237-e3001237 [ Pubmed: 33914735 DOI: doi:10.1371/journal.pbio.3001237 ]
- Vh ab8 (15 kDa, Protein from synthetic construct)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Spike glycoprotein (142 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Sars-cov-2 n501y mutant spike protein ectodomain bound to vh ab8 (Complex from Severe acute respiratory syndrome coronavirus 2)
Cryo-EM structure of the SARS-CoV-2 N501Y mutant spike protein ectodomain bound to Fab ab1 (class 1)
Resolution: 3.32 Å
EM Method: Single-particle
Fitted PDBs: 7mjj
Q-score: 0.316
Zhu X, Mannar D, Srivastava SS, Berezuk AM, Demers JP, Saville JW, Leopold K, Li W, Dimitrov DS, Tuttle KS, Zhou S, Chittori S, Subramaniam S
PLoS Biol (2021) 19 pp. e3001237-e3001237 [ Pubmed: 33914735 DOI: doi:10.1371/journal.pbio.3001237 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Spike glycoprotein (142 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Fab ab1 light chain (23 kDa, Protein from synthetic construct)
- Sars-cov-2 n501y mutant spike protein ectodomain bound to fab ab1 (Complex from Severe acute respiratory syndrome coronavirus 2)
- Fab ab1 heavy chain (26 kDa, Protein from synthetic construct)