Cryo-EM structure of FLVCR2 in the inward-facing state with choline bound
Resolution: 2.77 Å
EM Method: Single-particle
Fitted PDBs: 8vzn
Q-score: 0.583
Cater RJ, Mukherjee D, Gil-Iturbe E, Erramilli SK, Chen T, Koo K, Santander N, Reckers A, Kloss B, Gawda T, Choy BC, Zhang Z, Katewa A, Larpthaveesarp A, Huang EJ, Mooney SWJ, Clarke OB, Yee SW, Giacomini KM, Kossiakoff AA, Quick M, Arnold T, Mancia F
Nature (2024) 629 pp. 704-709 [ DOI: doi:10.1038/s41586-024-07326-y Pubmed: 38693257 ]
- Fab flv9 heavy chain (25 kDa, Protein from synthetic construct)
- Choline ion (104 Da, Ligand)
- Feline leukemia virus subgroup c cellular receptor 2 (60 kDa, Protein from Mus musculus)
- Fab flv9 light chain (23 kDa, Protein from synthetic construct)
- Flvcr2 in the inward-facing state complexed with choline and fab flv9 (110 kDa, Complex from Mus musculus)
Cryo-EM structure of FLVCR2 in the outward-facing state with choline bound
Resolution: 2.49 Å
EM Method: Single-particle
Fitted PDBs: 8vzo
Q-score: 0.637
Cater RJ, Mukherjee D, Gil-Iturbe E, Erramilli SK, Chen T, Koo K, Santander N, Reckers A, Kloss B, Gawda T, Choy BC, Zhang Z, Katewa A, Larpthaveesarp A, Huang EJ, Mooney SWJ, Clarke OB, Yee SW, Giacomini KM, Kossiakoff AA, Quick M, Arnold T, Mancia F
Nature (2024) 629 pp. 704-709 [ DOI: doi:10.1038/s41586-024-07326-y Pubmed: 38693257 ]
- Flvcr2 in the outward-facing state complexed with choline and fab flv23 (110 kDa, Complex from Mus musculus)
- Choline ion (104 Da, Ligand)
- Fab flv23 heavy chain (25 kDa, Protein from synthetic construct)
- Feline leukemia virus subgroup c cellular receptor 2 (60 kDa, Protein from Mus musculus)
- Fab flv23 light chain (23 kDa, Protein from synthetic construct)
Resolution: 2.92 Å
EM Method: Single-particle
Fitted PDBs: 8pm2
Q-score: 0.496
Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Verprintsev DB, Vaidehi N, Weyand SN, Tate CG
bioRxiv (2023) [ Pubmed: 37461561 DOI: doi:10.1101/2023.07.07.547762 ]
- Cholesterol hemisuccinate (486 Da, Ligand)
- ~{n},~{n}-dimethylcyclohexanamine (127 Da, Ligand)
- A complex of mouse trace-amine associated receptor 7f solubilized in lmng/chs bound to n,n-dimethylcyclohexylamine and coupled to: engineered guanine nucleotide-binding protein g(s) subunit alpha isoform short + guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 + guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 + nanobody 35 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (28 kDa, Protein from Homo sapiens)
- Nanobody 35 (16 kDa, Protein from Lama glama)
- Trace amine-associated receptor 7f (41 kDa, Protein from Mus musculus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)