Helical structure of BcThsA in complex with 1''-3'gcADPR
Resolution: 2.96 Å
EM Method: Helical reconstruction
Fitted PDBs: 8bto
Q-score: 0.535
Tamulaitiene G, Sabonis D, Sasnauskas G, Ruksenaite A, Silanskas A, Avraham C, Ofir G, Sorek R, Zaremba M, Siksnys V
Nature (2024) 627 pp. 431-436 [ DOI: doi:10.1038/s41586-024-07092-x Pubmed: 38383786 ]
- (2r,3r,3as,5s,6r,7s,8r,11r,13s,15ar)-2-(6-amino-9h-purin-9-yl)-3,6,7,11,13-pentahydroxyoctahydro-2h,5h,11h,13h-5,8-epoxy-11lambda~5~,13lambda~5~-furo[2,3-g][1,3,5,9,2,4]tetraoxadiphosphacyclotetradecine-11,13-dione (541 Da, Ligand)
- Nicotinamide-adenine-dinucleotide (663 Da, Ligand)
- Bcthsa in complex with 1''-3'gcadpr (Complex from Bacillus cereus)
- Nad(+) hydrolase thsa (55 kDa, Protein from Bacillus cereus MSX-D12)
Helical structure of BcThsA in complex with 1''-3'gc(etheno)ADPR
Resolution: 2.75 Å
EM Method: Helical reconstruction
Fitted PDBs: 8btp
Q-score: 0.577
Tamulaitiene G, Sabonis D, Sasnauskas G, Ruksenaite A, Silanskas A, Avraham C, Ofir G, Sorek R, Zaremba M, Siksnys V
Nature (2024) 627 pp. 431-436 [ DOI: doi:10.1038/s41586-024-07092-x Pubmed: 38383786 ]
- Bcthsa in complex with 1''-3'gc(etheno)adpr (Complex from Bacillus cereus)
- Nad(+) hydrolase thsa (55 kDa, Protein from Bacillus cereus MSX-D12)
- Etheno-nad (687 Da, Ligand)
- (1s,3s,4r,5r,7r,15r,16s,17r)-5-imidazo[2,1-f]purin-3-yl-10,12-bis(oxidanyl)-10,12-bis(oxidanylidene)-2,6,9,11,13,18-hexaoxa-10$l^{5},12$l^{5}-diphosphatricyclo[13.2.1.0^{3,7}]octadecane-4,16,17-triol (565 Da, Ligand)
Cryo-EM structure of the Cas12m-crRNA-target DNA complex
Resolution: 2.93 Å
EM Method: Single-particle
Fitted PDBs: 8pm4
Q-score: 0.542
Bigelyte G, Duchovska B, Zedaveinyte R, Sasnauskas G, Sinkunas T, Dalgediene I, Tamulaitiene G, Silanskas A, Kazlauskas D, Valancauskas L, Madariaga-Marcos J, Seidel R, Siksnys V, Karvelis T
Nucleic Acids Res (2024) 52 pp. 3234-3248 [ DOI: doi:10.1093/nar/gkae016 Pubmed: 38261981 ]
- Crrna, chain b (18 kDa, RNA from Gordonia otitidis NBRC 100426)
- Transposase (68 kDa, Protein from Gordonia otitidis NBRC 100426)
- Cas12m protein in complex with crrna and cognate dna, gordonia otitidis nbrc 100426 system (Complex from Gordonia otitidis NBRC 100426)
- Dna oligoduplex, non-target strand, chain d (13 kDa, DNA from synthetic construct)
- Dna oligoduplex, target strand, chain c (13 kDa, DNA from synthetic construct)
ISDra2 TnpB in complex with reRNA
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 8bf8
Q-score: 0.497
Sasnauskas G, Tamulaitiene G, Druteika G, Carabias A, Silanskas A, Kazlauskas D, Venclovas C, Montoya G, Karvelis T, Siksnys V
Nature (2023) 616 pp. 384-389 [ Pubmed: 37020015 DOI: doi:10.1038/s41586-023-05826-x ]
- Rna-guided dna endonuclease tnpb (46 kDa, Protein from Deinococcus radiodurans R1)
- Isdra2 tnpb binary complex (100 kDa, Complex from Deinococcus radiodurans)
- Deinococcus radiodurans r1 chromosome 1 (48 kDa, RNA from Deinococcus radiodurans R1)
ISDra2 TnpB in complex with reRNA and cognate DNA, conformation 2 (RuvC domain unresolved)
Resolution: 2.96 Å
EM Method: Single-particle
Fitted PDBs: 8ex9
Q-score: 0.45
Sasnauskas G, Tamulaitiene G, Druteika G, Carabias A, Silanskas A, Kazlauskas D, Venclovas C, Montoya G, Karvelis T, Siksnys V
Nature (2023) 616 pp. 384-389 [ Pubmed: 37020015 DOI: doi:10.1038/s41586-023-05826-x ]
- Rna-guided dna endonuclease tnpb (46 kDa, Protein from Deinococcus radiodurans R1)
- Rna (150-mer) (48 kDa, RNA from Deinococcus radiodurans R1)
- Isdra2 tnpb in complex with rerna and cognate dna (Complex from Deinococcus radiodurans R1)
- Dna (43-mer) (13 kDa, DNA from synthetic construct)
- Dna (43-mer) (13 kDa, DNA from synthetic construct)
ISDra2 TnpB in complex with reRNA and cognate DNA, conformation 1 (RuvC domain resolved)
Resolution: 3.14 Å
EM Method: Single-particle
Fitted PDBs: 8exa
Q-score: 0.441
Sasnauskas G, Tamulaitiene G, Druteika G, Carabias A, Silanskas A, Kazlauskas D, Venclovas C, Montoya G, Karvelis T, Siksnys V
Nature (2023) 616 pp. 384-389 [ Pubmed: 37020015 DOI: doi:10.1038/s41586-023-05826-x ]
- Rna-guided dna endonuclease tnpb (46 kDa, Protein from Deinococcus radiodurans R1)
- Isdra2 tnpb in complex with rerna and cognate dna (Complex from Deinococcus radiodurans R1)
- Dna (43-mer) (13 kDa, DNA from synthetic construct)
- Rna (150-mer) (48 kDa, RNA from Deinococcus radiodurans R1)
- Dna (43-mer) (13 kDa, DNA from synthetic construct)