CryoEM reconstruction of tri-specific 298-52-80 Multabody (no symmetry applied)
Burn Aschner C, Muthuraman K, Kucharska I, Cui H, Prieto K, Nair MS, Wang M, Huang Y, Christie-Holmes N, Poon B, Lam J, Sultana A, Kozak R, Mubareka S, Rubinstein JL, Rujas E, Treanor B, Ho DD, Jetha A, Julien JP
Sci Transl Med (2023) 15 pp. eadf4549-eadf4549 [ Pubmed: 37224226 DOI: doi:10.1126/scitranslmed.adf4549 ]
CryoEM reconstruction of the tri-specific 298-52-80 Multabody (octahedral symmetry applied)
Burn Aschner C, Muthuraman K, Kucharska I, Cui H, Prieto K, Nair MS, Wang M, Huang Y, Christie-Holmes N, Poon B, Lam J, Sultana A, Kozak R, Mubareka S, Rubinstein JL, Rujas E, Treanor B, Ho DD, Jetha A, Julien JP
Sci Transl Med (2023) 15 pp. eadf4549-eadf4549 [ Pubmed: 37224226 DOI: doi:10.1126/scitranslmed.adf4549 ]
Rujas E, Kucharska I, Tan YZ, Benlekbir S, Cui H, Zhao T, Wasney GA, Budylowski P, Guvenc F, Newton JC, Sicard T, Semesi A, Muthuraman K, Nouanesengsy A, Aschner CB, Prieto K, Bueler SA, Youssef S, Liao-Chan S, Glanville J, Christie-Holmes N, Mubareka S, Gray-Owen SD, Rubinstein JL, Treanor B, Julien JP
Nat Commun (2021) 12 pp. 3661-3661 [ DOI: doi:10.1038/s41467-021-23825-2 Pubmed: 34135340 ]
- Receptor binding domain of sars-cov-2 spike (Protein from Severe acute respiratory syndrome coronavirus 2)
- Fab fragment of neutralizing antibody 46 (Complex from Homo sapiens)
- Sars-cov-2 receptor binding domain (Complex from Severe acute respiratory syndrome coronavirus 2)
- Fab 46 light chain (Protein from Homo sapiens)
- Sars-cov-2 receptor binding domain in complex with the fab fragment of neutralizing antibody 46 (Complex)
- Fab 46 heavy chain (Protein from Homo sapiens)
Rujas E, Kucharska I, Tan YZ, Benlekbir S, Cui H, Zhao T, Wasney GA, Budylowski P, Guvenc F, Newton JC, Sicard T, Semesi A, Muthuraman K, Nouanesengsy A, Aschner CB, Prieto K, Bueler SA, Youssef S, Liao-Chan S, Glanville J, Christie-Holmes N, Mubareka S, Gray-Owen SD, Rubinstein JL, Treanor B, Julien JP
Nat Commun (2021) 12 pp. 3661-3661 [ DOI: doi:10.1038/s41467-021-23825-2 Pubmed: 34135340 ]
- Fab 80 light chain (Protein from Homo sapiens)
- Fab fragment of neutralizing antibody 80 (Complex from Homo sapiens)
- Sars-cov-2 spike (Complex from Severe acute respiratory syndrome coronavirus 2)
- Fab 80 heavy chain (Protein from Homo sapiens)
- Sars-cov-2 spike (Protein from Severe acute respiratory syndrome coronavirus 2)
- Sars-cov-2 spike in complex with the fab fragment of neutralizing antibody 80 (Complex)
Rujas E, Kucharska I, Tan YZ, Benlekbir S, Cui H, Zhao T, Wasney GA, Budylowski P, Guvenc F, Newton JC, Sicard T, Semesi A, Muthuraman K, Nouanesengsy A, Aschner CB, Prieto K, Bueler SA, Youssef S, Liao-Chan S, Glanville J, Christie-Holmes N, Mubareka S, Gray-Owen SD, Rubinstein JL, Treanor B, Julien JP
Nat Commun (2021) 12 pp. 3661-3661 [ DOI: doi:10.1038/s41467-021-23825-2 Pubmed: 34135340 ]
- Fab 298 heavy chain (Protein from Homo sapiens)
- Sars-cov-2 spike in complex with the fab fragment of neutralizing antibody 298 (Complex)
- Sars-cov-2 spike (Complex from Severe acute respiratory syndrome coronavirus 2)
- Fab 298 light chain (Protein from Homo sapiens)
- Fab fragment of neutralizing antibody 298 (Complex from Homo sapiens)
- Sars-cov-2 spike (Protein from Severe acute respiratory syndrome coronavirus 2)
Rujas E, Kucharska I, Tan YZ, Benlekbir S, Cui H, Zhao T, Wasney GA, Budylowski P, Guvenc F, Newton JC, Sicard T, Semesi A, Muthuraman K, Nouanesengsy A, Aschner CB, Prieto K, Bueler SA, Youssef S, Liao-Chan S, Glanville J, Christie-Holmes N, Mubareka S, Gray-Owen SD, Rubinstein JL, Treanor B, Julien JP
Nat Commun (2021) 12 pp. 3661-3661 [ DOI: doi:10.1038/s41467-021-23825-2 Pubmed: 34135340 ]
- Sars-cov-2 spike (Complex from Severe acute respiratory syndrome coronavirus 2)
- Fab 324 heavy chain (Protein from Homo sapiens)
- Fab 324 light chain (Protein from Homo sapiens)
- Sars-cov-2 spike in complex with the fab fragment of neutralizing antibody 324 (Complex)
- Sars-cov-2 spike (Protein from Severe acute respiratory syndrome coronavirus 2)
- Fab fragment of neutralizing antibody 324 (Complex from Homo sapiens)
CryoEM structure of the C. sordellii lethal toxin TcsL in complex with SEMA6A
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 6wts
Q-score: 0.463
Lee H, Beilhartz GL, Kucharska I, Raman S, Cui H, Lam MHY, Liang H, Rubinstein JL, Schramek D, Julien JP, Melnyk RA, Taipale M
Cell (2020) 182 pp. 345- [ Pubmed: 32589945 DOI: doi:10.1016/j.cell.2020.06.005 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Tcsl (Complex from Paeniclostridium sordellii)
- Sema6a (63 kDa, Protein from Homo sapiens)
- Tcsl (63 kDa, Protein from Paeniclostridium sordellii)
- Sema6a (Complex from Homo sapiens)
- Complex of sema6a with tcsl (Complex)
CryoEM structure of human SEMA6A dimer
Lee H, Beilhartz GL, Kucharska I, Raman S, Cui H, Lam MHY, Liang H, Rubinstein JL, Schramek D, Julien JP, Melnyk RA, Taipale M
Cell (2020) [ Pubmed: 32589945 DOI: doi:10.1016/j.cell.2020.06.005 ]
- Sema6a (Protein from Homo sapiens)
- Sema6a dimer (Cell from Homo sapiens)