Outer kinetochore Ndc80-Dam1 alpha/beta-tubulin complex
Resolution: 3.54 Å
EM Method: Single-particle
Fitted PDBs: 8qau
Q-score: 0.343
Muir KW, Batters C, Dendooven T, Yang J, Zhang Z, Burt A, Barford D
bioRxiv (2023) [ DOI: doi:10.1101/2023.07.20.549907 ]
- Kinetochore protein nuf2 (53 kDa, Protein from Saccharomyces cerevisiae)
- Tubulin alpha-1a chain (50 kDa, Protein from Sus scrofa)
- Taxol (853 Da, Ligand)
- Tubulin beta chain (49 kDa, Protein from Sus scrofa)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Dash complex subunit dam1 (38 kDa, Protein from Saccharomyces cerevisiae)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Kinetochore protein ndc80 (80 kDa, Protein from Saccharomyces cerevisiae)
- Magnesium ion (24 Da, Ligand)
- Outer kinetochore ndc80-nuf2 ch domains bound to dam1 in complex with alpha-beta tubulin (Complex from Saccharomyces cerevisiae)
Muir KW, Batters C, Dendooven T, Yang J, Zhang Z, Burt A, Barford D
Science (2023) 382 pp. 1184-1190 [ DOI: doi:10.1126/science.adj8736 Pubmed: 38060647 ]
Outer kinetochore Dam1 protomer dimer Ndc80-Nuf2 coiled-coil complex
Resolution: 3.15 Å
EM Method: Single-particle
Fitted PDBs: 8q84
Q-score: 0.294
Muir KW, Batters C, Dendooven T, Yang J, Zhang Z, Burt A, Barford D
Science (2023) 382 pp. 1184-1190 [ DOI: doi:10.1126/science.adj8736 Pubmed: 38060647 ]
- Dash complex subunit dad1 (10 kDa, Protein from Saccharomyces cerevisiae)
- Dash complex subunit dad2 (15 kDa, Protein from Saccharomyces cerevisiae)
- Dash complex subunit spc34 (34 kDa, Protein from Saccharomyces cerevisiae)
- Dash complex subunit dad4 (8 kDa, Protein from Saccharomyces cerevisiae)
- Dash complex subunit hsk3 (8 kDa, Protein from Saccharomyces cerevisiae)
- Kinetochore protein nuf2 (53 kDa, Protein from Saccharomyces cerevisiae)
- Dash complex subunit duo1 (27 kDa, Protein from Saccharomyces cerevisiae)
- Dash complex subunit ask1 (32 kDa, Protein from Saccharomyces cerevisiae)
- Dash complex subunit dam1 (38 kDa, Protein from Saccharomyces cerevisiae)
- Dash complex subunit dad3 (10 kDa, Protein from Saccharomyces cerevisiae)
- Kinetochore protein ndc80 (80 kDa, Protein from Saccharomyces cerevisiae)
- Outer kinetochore dam1 protomer dimer with staple and ndc80-nuf2 coiled-coils (673 GDa, Complex from Saccharomyces cerevisiae)
- Dash complex subunit spc19 (18 kDa, Protein from Saccharomyces cerevisiae)
Outer kinetochore Dam1 protomer monomer Ndc80-Nuf2 coiled-coil complex
Resolution: 3.97 Å
EM Method: Single-particle
Fitted PDBs: 8q85
Q-score: 0.277
Muir KW, Batters C, Dendooven T, Yang J, Zhang Z, Burt A, Barford D
Science (2023) 382 pp. 1184-1190 [ DOI: doi:10.1126/science.adj8736 Pubmed: 38060647 ]
- Outer kinetochore dam1 protomer monomer with staple and ndc80-nuf2 coiled-coils (673 GDa, Complex from Saccharomyces cerevisiae)
- Kinetochore protein nuf2 (53 kDa, Protein from Saccharomyces cerevisiae)
- Dash complex subunit spc34 (34 kDa, Protein from Saccharomyces cerevisiae)
- Dash complex subunit ask1 (32 kDa, Protein from Saccharomyces cerevisiae)
- Dash complex subunit dad1 (10 kDa, Protein from Saccharomyces cerevisiae)
- Dash complex subunit dad3 (10 kDa, Protein from Saccharomyces cerevisiae)
- Dash complex subunit spc19 (18 kDa, Protein from Saccharomyces cerevisiae)
- Dash complex subunit hsk3 (8 kDa, Protein from Saccharomyces cerevisiae)
- Dash complex subunit dam1 (38 kDa, Protein from Saccharomyces cerevisiae)
- Kinetochore protein ndc80 (80 kDa, Protein from Saccharomyces cerevisiae)
- Dash complex subunit duo1 (27 kDa, Protein from Saccharomyces cerevisiae)
- Dash complex subunit dad4 (8 kDa, Protein from Saccharomyces cerevisiae)
- Dash complex subunit dad2 (15 kDa, Protein from Saccharomyces cerevisiae)
Structure of the human CCAN CENP-A alpha-satellite complex
Resolution: 2.68 Å
EM Method: Single-particle
Fitted PDBs: 7pii
Q-score: 0.573
Yatskevich S, Muir KW, Bellini D, Zhang Z, Yang J, Tischer T, Predin M, Dendooven T, McLaughlin SH, Barford D
Science (2022) 376 pp. 844-852 [ DOI: doi:10.1126/science.abn3810 Pubmed: 35420891 ]
- Dna (122-mer) (52 kDa, DNA from Homo sapiens)
- Histone h4 (11 kDa, Protein from Homo sapiens)
- Histone h2b type 1-c/e/f/g/i (13 kDa, Protein from Homo sapiens)
- Histone h2a type 1-c (16 kDa, Protein from Homo sapiens)
- Histone h3-like centromeric protein a (16 kDa, Protein from Homo sapiens)
- Centromere protein c (61 kDa, Protein from Homo sapiens)
- Cenp-a nucleosome in complex with cenp-c (Complex)
- Dna (123-mer) (52 kDa, DNA from Homo sapiens)
Structure of the human CCAN CENP-A alpha-satellite complex
Resolution: 12.0 Å
EM Method: Single-particle
Fitted PDBs: 7ywx
Q-score: 0.054
Yatskevich S, Muir KW, Bellini D, Zhang Z, Yang J, Tischer T, Predin M, Dendooven T, McLaughlin SH, Barford D
Science (2022) 376 pp. 844-852 [ DOI: doi:10.1126/science.abn3810 Pubmed: 35420891 ]
- Histone h4 (11 kDa, Protein from Homo sapiens)
- Centromere protein o (33 kDa, Protein from Homo sapiens)
- Histone h2a type 1-c (14 kDa, Protein from Homo sapiens)
- Centromere protein w (10 kDa, Protein from Homo sapiens)
- Centromere protein r (20 kDa, Protein from Homo sapiens)
- Histone h3-like centromeric protein a (16 kDa, Protein from Homo sapiens)
- Centromere protein l (39 kDa, Protein from Homo sapiens)
- Centromere protein i (86 kDa, Protein from Homo sapiens)
- Dna (171-mer) (52 kDa, DNA from Homo sapiens)
- Centromere protein s (15 kDa, Protein from Homo sapiens)
- Centromere protein x (8 kDa, Protein from Homo sapiens)
- Ccan-cenp-a inner centromere complex (Complex)
- Centromere protein n (39 kDa, Protein from Homo sapiens)
- Centromere protein m (19 kDa, Protein from Homo sapiens)
- Centromere protein k (31 kDa, Protein from Homo sapiens)
- Centromere protein u (47 kDa, Protein from Homo sapiens)
- Centromere protein c (61 kDa, Protein from Homo sapiens)
- Centromere protein h (28 kDa, Protein from Homo sapiens)
- Centromere protein q (30 kDa, Protein from Homo sapiens)
- Centromere protein p (33 kDa, Protein from Homo sapiens)
- Histone h2b type 1-c/e/f/g/i (13 kDa, Protein from Homo sapiens)
- Dna (171-mer) (52 kDa, DNA from Homo sapiens)
- Centromere protein t (60 kDa, Protein from Homo sapiens)
Structure of the human CCANdeltaT CENP-A alpha-satellite complex
Resolution: 8.9 Å
EM Method: Single-particle
Fitted PDBs: 7yyh
Q-score: 0.075
Yatskevich S, Muir KW, Bellini D, Zhang Z, Yang J, Tischer T, Predin M, Dendooven T, McLaughlin SH, Barford D
Science (2022) 376 pp. 844-852 [ DOI: doi:10.1126/science.abn3810 Pubmed: 35420891 ]
- Centromere protein u (47 kDa, Protein from Homo sapiens)
- Histone h3-like centromeric protein a (16 kDa, Protein from Homo sapiens)
- Centromere protein c (61 kDa, Protein from Homo sapiens)
- Dna (171-mer) (52 kDa, DNA from Homo sapiens)
- Centromere protein r (20 kDa, Protein from Homo sapiens)
- Centromere protein h (28 kDa, Protein from Homo sapiens)
- Centromere protein n (39 kDa, Protein from Homo sapiens)
- Histone h4 (11 kDa, Protein from Homo sapiens)
- Dna (171-mer) (52 kDa, DNA from Homo sapiens)
- Histone h2b type 1-c/e/f/g/i (13 kDa, Protein from Homo sapiens)
- Centromere protein l (39 kDa, Protein from Homo sapiens)
- Centromere protein o (33 kDa, Protein from Homo sapiens)
- Centromere protein p (33 kDa, Protein from Homo sapiens)
- Histone h2a type 1-c (14 kDa, Protein from Homo sapiens)
- Centromere protein q (30 kDa, Protein from Homo sapiens)
- Centromere protein i (86 kDa, Protein from Homo sapiens)
- Centromere protein m (19 kDa, Protein from Homo sapiens)
- Ccan-cenp-a inner centromere complex (Complex)
- Centromere protein k (31 kDa, Protein from Homo sapiens)
Structure of the human CCAN deltaCT complex
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7pkn
Q-score: 0.319
Yatskevich S, Muir KW, Bellini D, Zhang Z, Yang J, Tischer T, Predin M, Dendooven T, McLaughlin SH, Barford D
Science (2022) 376 pp. 844-852 [ DOI: doi:10.1126/science.abn3810 Pubmed: 35420891 ]
- Centromere protein q (30 kDa, Protein from Homo sapiens)
- Centromere protein p (33 kDa, Protein from Homo sapiens)
- Centromere protein l (39 kDa, Protein from Homo sapiens)
- Centromere protein k (31 kDa, Protein from Homo sapiens)
- Centromere protein u (47 kDa, Protein from Homo sapiens)
- Human inner kinetochore ccan (Complex from Homo sapiens)
- Centromere protein i (86 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Centromere protein o (33 kDa, Protein from Homo sapiens)
- Centromere protein n (39 kDa, Protein from Homo sapiens)
- Centromere protein h (28 kDa, Protein from Homo sapiens)
- Centromere protein m (19 kDa, Protein from Homo sapiens)
- Centromere protein r (20 kDa, Protein from Homo sapiens)
Structure of the human CCAN CENP-A alpha-satellite complex
Resolution: 2.44 Å
EM Method: Single-particle
Fitted PDBs: 7r5r
Q-score: 0.571
Yatskevich S, Muir KW, Bellini D, Zhang Z, Yang J, Tischer T, Predin M, Dendooven T, McLaughlin SH, Barford D
Science (2022) 376 pp. 844-852 [ DOI: doi:10.1126/science.abn3810 Pubmed: 35420891 ]
- Histone h4 (11 kDa, Protein from Homo sapiens)
- Histone h2b type 1-c/e/f/g/i (13 kDa, Protein from Homo sapiens)
- Dna (171-mer) (52 kDa, DNA from Homo sapiens)
- Histone h3-like centromeric protein a (16 kDa, Protein from Homo sapiens)
- Histone h2a type 1-c (14 kDa, Protein from Homo sapiens)
- Dna (171-mer) (52 kDa, DNA from Homo sapiens)
- Centromere protein c (61 kDa, Protein from Homo sapiens)
- Human ccan complex with bound alpha-satellite dna (Complex)
Structure of the human CCAN bound to alpha satellite DNA
Resolution: 2.83 Å
EM Method: Single-particle
Fitted PDBs: 7r5s
Q-score: 0.465
Yatskevich S, Muir KW, Bellini D, Zhang Z, Yang J, Tischer T, Predin M, Dendooven T, McLaughlin SH, Barford D
Science (2022) 376 pp. 844-852 [ DOI: doi:10.1126/science.abn3810 Pubmed: 35420891 ]
- Dna (66-mer) (16 kDa, DNA from Homo sapiens)
- Centromere protein o (33 kDa, Protein from Homo sapiens)
- Centromere protein w (10 kDa, Protein from Homo sapiens)
- Centromere protein r (20 kDa, Protein from Homo sapiens)
- Centromere protein l (39 kDa, Protein from Homo sapiens)
- Centromere protein i (86 kDa, Protein from Homo sapiens)
- Centromere protein s (15 kDa, Protein from Homo sapiens)
- Centromere protein x (8 kDa, Protein from Homo sapiens)
- Centromere protein n (39 kDa, Protein from Homo sapiens)
- Centromere protein m (19 kDa, Protein from Homo sapiens)
- Centromere protein k (31 kDa, Protein from Homo sapiens)
- Centromere protein u (47 kDa, Protein from Homo sapiens)
- Centromere protein h (28 kDa, Protein from Homo sapiens)
- Dna (66-mer) (16 kDa, DNA from Homo sapiens)
- Centromere protein q (30 kDa, Protein from Homo sapiens)
- Centromere protein p (33 kDa, Protein from Homo sapiens)
- Human ccan complex with bound alpha-satellite dna (Complex)
- Water (18 Da, Ligand)
- Centromere protein t (60 kDa, Protein from Homo sapiens)