Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 6pb0
Q-score: 0.455
Ma S, Shen Q, Zhao LH, Mao C, Zhou XE, Shen DD, de Waal PW, Bi P, Li C, Jiang Y, Wang MW, Sexton PM, Wootten D, Melcher K, Zhang Y, Xu HE
Mol Cell (2020) 77 pp. 669- [ Pubmed: 32004470 DOI: doi:10.1016/j.molcel.2020.01.013 ]
- Urocortin (4 kDa, Protein from Homo sapiens)
- Nanobody 35 (15 kDa, Protein from synthetic construct)
- Palmitic acid (256 Da, Ligand)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short,guanine nucleotide-binding protein g(i) subunit alpha-1,guanine nucleotide-binding protein g(s) subunit alpha isoforms short (43 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- Urocortin1-bound crf1r in complex gs and nb35 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Corticotropin-releasing factor receptor 1 (43 kDa, Protein from Homo sapiens)
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 6pb1
Q-score: 0.47
Ma S, Shen Q, Zhao LH, Mao C, Zhou XE, Shen DD, de Waal PW, Bi P, Li C, Jiang Y, Wang MW, Sexton PM, Wootten D, Melcher K, Zhang Y, Xu HE
Mol Cell (2020) 77 pp. 669- [ Pubmed: 32004470 DOI: doi:10.1016/j.molcel.2020.01.013 ]
- Urocortin (4 kDa, Protein from Homo sapiens)
- Corticotropin-releasing factor receptor 2 (45 kDa, Protein from Homo sapiens)
- Urocortin 1-bound corticotropin-releasing factor 2 receptor in complex with gs protein (Complex from Homo sapiens)
- Palmitic acid (256 Da, Ligand)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short,guanine nucleotide-binding protein g(i) subunit alpha-1,guanine nucleotide-binding protein g(s) subunit alpha isoforms short (43 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- Nanobody 35 (15 kDa, Protein from synthetic construct)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 6nbf
Q-score: 0.444
Zhao LH, Ma S, Sutkeviciute I, Shen DD, Zhou XE, de Waal PW, Li CY, Kang Y, Clark LJ, Jean-Alphonse FG, White AD, Yang D, Dai A, Cai X, Chen J, Li C, Jiang Y, Watanabe T, Gardella TJ, Melcher K, Wang MW, Vilardaga JP, Xu HE, Zhang Y
Science (2019) 364 pp. 148-153 [ DOI: doi:10.1126/science.aav7942 Pubmed: 30975883 ]
- Cholesterol (386 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Bos taurus)
- Palmitic acid (256 Da, Ligand)
- Long-acting parathyroid hormone analog (4 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Rattus norvegicus)
- Gs protein alpha subunit (43 kDa, Protein from Bos taurus)
- Nanobody-35 (13 kDa, Protein from synthetic construct)
- Cryo-em structure of parathyroid hormone receptor type 1 in complex with a long-acting parathyroid hormone analog and g protein (Complex from Homo sapiens)
- Parathyroid hormone/parathyroid hormone-related peptide receptor (54 kDa, Protein from Homo sapiens)
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 6nbh
Q-score: 0.347
Zhao LH, Ma S, Sutkeviciute I, Shen DD, Zhou XE, de Waal PW, Li CY, Kang Y, Clark LJ, Jean-Alphonse FG, White AD, Yang D, Dai A, Cai X, Chen J, Li C, Jiang Y, Watanabe T, Gardella TJ, Melcher K, Wang MW, Vilardaga JP, Xu HE, Zhang Y
Science (2019) 364 pp. 148-153 [ DOI: doi:10.1126/science.aav7942 Pubmed: 30975883 ]
- Cholesterol (386 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Bos taurus)
- Palmitic acid (256 Da, Ligand)
- Long-acting parathyroid hormone analog (4 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Rattus norvegicus)
- Gs protein alpha subunit (43 kDa, Protein from Bos taurus)
- Nanobody-35 (13 kDa, Protein from synthetic construct)
- Cryo-em structure of parathyroid hormone receptor type 1 in complex with a long-acting parathyroid hormone analog and g protein (Complex from Homo sapiens)
- Parathyroid hormone/parathyroid hormone-related peptide receptor (54 kDa, Protein from Homo sapiens)
Resolution: 4.0 Å
EM Method: Single-particle
Fitted PDBs: 6nbi
Q-score: 0.285
Zhao LH, Ma S, Sutkeviciute I, Shen DD, Zhou XE, de Waal PW, Li CY, Kang Y, Clark LJ, Jean-Alphonse FG, White AD, Yang D, Dai A, Cai X, Chen J, Li C, Jiang Y, Watanabe T, Gardella TJ, Melcher K, Wang MW, Vilardaga JP, Xu HE, Zhang Y
Science (2019) 364 pp. 148-153 [ DOI: doi:10.1126/science.aav7942 Pubmed: 30975883 ]
- Cholesterol (386 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Bos taurus)
- Long-acting parathyroid hormone analog (4 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Rattus norvegicus)
- Gs protein alpha subunit (43 kDa, Protein from Bos taurus)
- Nanobody-35 (13 kDa, Protein from synthetic construct)
- Cryo-em structure of parathyroid hormone receptor type 1 in complex with a long-acting parathyroid hormone analog and g protein (Complex from Homo sapiens)
- Parathyroid hormone/parathyroid hormone-related peptide receptor (54 kDa, Protein from Homo sapiens)