Single particle cryo-EM of H. thermophilus chaperonin complex
Liao Z, Gopalasingam CC, Kameya M, Gerle C, Shigematsu H, Ishii M, Arakawa T, Fushinobu S
Structure (London, England : 1993) (2024) [ DOI: 10.1016/j.str.2024.02.012 Pubmed: 38492570 ]
Image sets:
Single particle cryo-EM of H. thermoluteolus chaperonin complex
Liao Z, Gopalasingam CC, Kameya M, Gerle C, Shigematsu H, Ishii M, Arakawa T, Fushinobu S
Structure (London, England : 1993) (2024) [ DOI: 10.1016/j.str.2024.02.012 Pubmed: 38492570 ]
Image sets:
Cryo-EM structure of native H. thermoluteolus TH-1 GroEL
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 8wu4
Q-score: 0.44
Liao Z, Gopalasingam CC, Kameya M, Gerle C, Shigematsu H, Ishii M, Arakawa T, Fushinobu S
Structure (2024) 32 pp. 679-689.e4 [ DOI: doi:10.1016/j.str.2024.02.012 Pubmed: 38492570 ]
- Native h. thermoluteolus groel (809 MDa, Complex from Hydrogenophilus thermoluteolus)
- Chaperonin groel (55 kDa, Protein from Hydrogenophilus thermoluteolus)
Cryo-EM structure of H. thermoluteolus GroEL-GroES2 football complex
Resolution: 2.5 Å
EM Method: Single-particle
Fitted PDBs: 8wuc
Q-score: 0.554
Liao Z, Gopalasingam CC, Kameya M, Gerle C, Shigematsu H, Ishii M, Arakawa T, Fushinobu S
Structure (2024) 32 pp. 679-689.e4 [ DOI: doi:10.1016/j.str.2024.02.012 Pubmed: 38492570 ]
- Co-chaperonin groes (10 kDa, Protein from Hydrogenophilus thermoluteolus)
- H. thermoluteolus groel-groes2 football complex bound with adp-mg2+ (73 MDa, Complex from Hydrogenophilus thermoluteolus)
- Magnesium ion (24 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- H. thermoluteolus groes (Complex)
- Chaperonin groel (56 kDa, Protein from Hydrogenophilus thermoluteolus)
- H. thermoluteolus groel (Complex)
Cryo-EM structure of H. thermophilus GroEL-GroES2 asymmetric football complex
Resolution: 2.6 Å
EM Method: Single-particle
Fitted PDBs: 8wuw
Q-score: 0.444
Liao Z, Gopalasingam CC, Kameya M, Gerle C, Shigematsu H, Ishii M, Arakawa T, Fushinobu S
Structure (2024) 32 pp. 679-689.e4 [ DOI: doi:10.1016/j.str.2024.02.012 Pubmed: 38492570 ]
- Potassium ion (39 Da, Ligand)
- Chaperonin groel (Complex)
- Magnesium ion (24 Da, Ligand)
- Chaperonin groel (56 kDa, Protein from Hydrogenobacter thermophilus TK-6)
- Co-chaperonin groes (10 kDa, Protein from Hydrogenobacter thermophilus TK-6)
- H. thermophilus groel-groes2 asymmetric football complex (Complex from Hydrogenobacter thermophilus TK-6)
- Phosphoaminophosphonic acid-adenylate ester (506 Da, Ligand)
- Co-chaperonin groes (Complex)
Cryo-EM structure of H. thermophilus GroEL-GroES bullet complex
Resolution: 2.6 Å
EM Method: Single-particle
Fitted PDBs: 8wux
Q-score: 0.453
Liao Z, Gopalasingam CC, Kameya M, Gerle C, Shigematsu H, Ishii M, Arakawa T, Fushinobu S
Structure (2024) 32 pp. 679-689.e4 [ DOI: doi:10.1016/j.str.2024.02.012 Pubmed: 38492570 ]
- Co-chaperonin groes (Complex)
- Potassium ion (39 Da, Ligand)
- Co-chaperonin groes (10 kDa, Protein from Hydrogenobacter thermophilus TK-6)
- Magnesium ion (24 Da, Ligand)
- Chaperonin groel (56 kDa, Protein from Hydrogenobacter thermophilus TK-6)
- H. thermophilus groel-groes bullet complex (Complex from Hydrogenobacter thermophilus TK-6)
- Phosphoaminophosphonic acid-adenylate ester (506 Da, Ligand)
- Chaperonin groel (Complex)
Cryo-EM structure of the gastric proton pump with bound DQ-02
Resolution: 2.1 Å
EM Method: Single-particle
Fitted PDBs: 8ijv
Q-score: 0.687
Abe K, Ozako M, Inukai M, Matsuyuki Y, Kitayama S, Kanai C, Nagai C, Gopalasingam CC, Gerle C, Shigematsu H, Umekubo N, Yokoshima S, Yoshimori A
Commun Biol (2023) 6 pp. 956-956 [ Pubmed: 37726448 DOI: doi:10.1038/s42003-023-05334-8 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- 1,2-dioleoyl-sn-glycero-3-phosphocholine (787 Da, Ligand)
- Water (18 Da, Ligand)
- Cholesterol (386 Da, Ligand)
- 4-[[5-chloranyl-2-(4-chlorophenyl)phenyl]methoxy]-n-methyl-but-2-yn-1-amine (334 Da, Ligand)
- Sodium/potassium-transporting atpase subunit alpha (114 kDa, Protein from Sus scrofa)
- Magnesium ion (24 Da, Ligand)
- Hetero dimer of alpha and beta subunit (135 kDa, Complex from Sus scrofa)
- Potassium-transporting atpase subunit beta (33 kDa, Protein from Sus scrofa)
Cryo-EM structure of the gastric proton pump with bound DQ-06
Resolution: 2.19 Å
EM Method: Single-particle
Fitted PDBs: 8ijw
Q-score: 0.658
Abe K, Ozako M, Inukai M, Matsuyuki Y, Kitayama S, Kanai C, Nagai C, Gopalasingam CC, Gerle C, Shigematsu H, Umekubo N, Yokoshima S, Yoshimori A
Commun Biol (2023) 6 pp. 956-956 [ Pubmed: 37726448 DOI: doi:10.1038/s42003-023-05334-8 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- 1,2-dioleoyl-sn-glycero-3-phosphocholine (787 Da, Ligand)
- N-methyl-1-[4-[(2-phenylmethoxycyclohexa-1,3-dien-1-yl)methoxy]cyclohexa-1,3-dien-1-yl]methanamine (337 Da, Ligand)
- Water (18 Da, Ligand)
- Cholesterol (386 Da, Ligand)
- Sodium/potassium-transporting atpase subunit alpha (114 kDa, Protein from Sus scrofa)
- Magnesium ion (24 Da, Ligand)
- Hetero dimer of alpha and beta subunit (135 kDa, Complex from Sus scrofa)
- Potassium-transporting atpase subunit beta (33 kDa, Protein from Sus scrofa)
Cryo-EM structure of the gastric proton pump with bound DQ-18
Resolution: 2.08 Å
EM Method: Single-particle
Fitted PDBs: 8ijx
Q-score: 0.68
Abe K, Ozako M, Inukai M, Matsuyuki Y, Kitayama S, Kanai C, Nagai C, Gopalasingam CC, Gerle C, Shigematsu H, Umekubo N, Yokoshima S, Yoshimori A
Commun Biol (2023) 6 pp. 956-956 [ Pubmed: 37726448 DOI: doi:10.1038/s42003-023-05334-8 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- 1,2-dioleoyl-sn-glycero-3-phosphocholine (787 Da, Ligand)
- Water (18 Da, Ligand)
- Cholesterol (386 Da, Ligand)
- 1-[4-[(5-chloranyl-2-phenylmethoxy-phenyl)methoxy]phenyl]-n-methyl-methanamine (367 Da, Ligand)
- Sodium/potassium-transporting atpase subunit alpha (114 kDa, Protein from Sus scrofa)
- Magnesium ion (24 Da, Ligand)
- Hetero dimer of alpha and beta subunit (135 kDa, Complex from Sus scrofa)
- Potassium-transporting atpase subunit beta (33 kDa, Protein from Sus scrofa)
Cryo-EM structure of the gastric proton pump with bound DQ-21
Resolution: 2.26 Å
EM Method: Single-particle
Fitted PDBs: 8jmn
Q-score: 0.67
Abe K, Ozako M, Inukai M, Matsuyuki Y, Kitayama S, Kanai C, Nagai C, Gopalasingam CC, Gerle C, Shigematsu H, Umekubo N, Yokoshima S, Yoshimori A
Commun Biol (2023) 6 pp. 956-956 [ Pubmed: 37726448 DOI: doi:10.1038/s42003-023-05334-8 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- 1,2-dioleoyl-sn-glycero-3-phosphocholine (787 Da, Ligand)
- 1-[4-[[2-[(4-chlorophenyl)methoxy]phenyl]methoxy]phenyl]-n-methyl-methanamine (367 Da, Ligand)
- Water (18 Da, Ligand)
- Cholesterol (386 Da, Ligand)
- Sodium/potassium-transporting atpase subunit alpha (114 kDa, Protein from Sus scrofa)
- Magnesium ion (24 Da, Ligand)
- Hetero dimer of alpha and beta subunit (135 kDa, Complex from Sus scrofa)
- Potassium-transporting atpase subunit beta (33 kDa, Protein from Sus scrofa)