Cryo-EM structure of C.crescentus bNY30a pilus complex
Resolution: 0.86 Å
EM Method: Helical reconstruction
Fitted PDBs: 8u2b
Q-score: 0.521
Wang Y, Theodore M, Xing Z, Narsaria U, Yu Z, Zeng L, Zhang J
Sci Adv (2024) 10 pp. eadl4450-eadl4450 [ Pubmed: 38701202 DOI: doi:10.1126/sciadv.adl4450 ]
- Flp family type ivb pilin (4 kDa, Protein from Caulobacter vibrioides)
- Caulobacter crescentus pili (170 kDa, Complex from Caulobacter vibrioides)
PhiCb5 maturation protein with Caulobacter crescentus bNY30a pili
Resolution: 6.45 Å
EM Method: Single-particle
Fitted PDBs: 8ucr
Q-score: 0.158
Wang Y, Theodore M, Xing Z, Narsaria U, Yu Z, Zeng L, Zhang J
Sci Adv (2024) 10 pp. eadl4450-eadl4450 [ Pubmed: 38701202 DOI: doi:10.1126/sciadv.adl4450 ]
- Maturation protein (40 kDa, Protein from Caulobacter phage phiCb5)
- A complex of pilus and phicb5 maturation protein (210 kDa, Complex from Caulobacter vibrioides)
- Flp family type ivb pilin (4 kDa, Protein from Caulobacter vibrioides)
Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 8uej
Q-score: 0.449
Wang Y, Theodore M, Xing Z, Narsaria U, Yu Z, Zeng L, Zhang J
Sci Adv (2024) 10 pp. eadl4450-eadl4450 [ Pubmed: 38701202 DOI: doi:10.1126/sciadv.adl4450 ]
- Maturation protein (40 kDa, Protein from Caulobacter phage phiCb5)
- A complex of phicb5 maturation protein and phicb5 shell (2 MDa, Complex from Caulobacter vibrioides)
- Coat protein (13 kDa, Protein from Caulobacter phage phiCb5)
- Calcium ion (40 Da, Ligand)
Type IV pilus from Pseudomonas PAO1 strain
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 8tum
Q-score: 0.448
Thongchol J, Yu Z, Harb L, Lin Y, Koch M, Theodore M, Narsaria U, Shaevitz J, Gitai Z, Wu Y, Zhang J, Zeng L
Science (2024) 384 pp. eadl0635-eadl0635 [ Pubmed: 38574145 DOI: doi:10.1126/science.adl0635 ]
- Type iv twitching pilus (240 kDa, Cellular component from Pseudomonas aeruginosa)
- Type iv major pilin protein pila (14 kDa, Protein from Pseudomonas aeruginosa PAO1)
Asymmetric reconstruction of mature PP7 virions
Thongchol J, Yu Z, Harb L, Lin Y, Koch M, Theodore M, Narsaria U, Shaevitz J, Gitai Z, Wu Y, Zhang J, Zeng L
Science (2024) 384 pp. eadl0635-eadl0635 [ DOI: doi:10.1126/science.adl0635 Pubmed: 38574145 ]
- Type iv twitching pilus (240 kDa, Cellular component from Pseudomonas aeruginosa)
Type IV pilus from Pseudomonas PAO1 strain with PP7 Maturation protein
Resolution: 7.9 Å
EM Method: Single-particle
Fitted PDBs: 8tuw
Q-score: 0.087
Thongchol J, Yu Z, Harb L, Lin Y, Koch M, Theodore M, Narsaria U, Shaevitz J, Gitai Z, Wu Y, Zhang J, Zeng L
Science (2024) 384 pp. eadl0635-eadl0635 [ Pubmed: 38574145 DOI: doi:10.1126/science.adl0635 ]
- Type iv twitching pilus (240 kDa, Cellular component from Pseudomonas aeruginosa)
- Maturation protein a (50 kDa, Protein from Pseudomonas phage PP7)
- Type iv major pilin protein pila (14 kDa, Protein from Pseudomonas aeruginosa PAO1)
The original consensus map of PP7 binding to T4P from PAO1
Thongchol J, Yu Z, Harb L, Lin Y, Koch M, Theodore M, Narsaria U, Shaevitz J, Gitai Z, Wu Y, Zhang J, Zeng L
Science (2024) 384 pp. eadl0635-eadl0635 [ DOI: doi:10.1126/science.adl0635 Pubmed: 38574145 ]
- Type iv twitching pilus (240 kDa, Cellular component from Pseudomonas aeruginosa)
Composite map of PP7 binding to type IV pilus from PAO1
Thongchol J, Yu Z, Harb L, Lin Y, Koch M, Theodore M, Narsaria U, Shaevitz J, Gitai Z, Wu Y, Zhang J, Zeng L
Science (2024) 384 pp. eadl0635-eadl0635 [ Pubmed: 38574145 DOI: doi:10.1126/science.adl0635 ]
- Type iv twitching pilus (240 kDa, Cellular component from Pseudomonas aeruginosa)
Cryo-EM structure of the EfPiwi (N959K)-piRNA-target ternary complex
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7yfq
Q-score: 0.435
Li Z, Li Z, Zhang Y, Zhou L, Xu Q, Li L, Zeng L, Xue J, Niu H, Zhong J, Yu Q, Li D, Gui M, Huang Y, Tu S, Zhang Z, Song CQ, Wu J, Shen EZ
Nat Struct Mol Biol (2024) [ Pubmed: 38658622 DOI: doi:10.1038/s41594-024-01287-6 ]
- Pirna (7 kDa, RNA from Homo sapiens)
- Piwi (92 kDa, Protein from Ephydatia fluviatilis)
- Rna (5'-r(*up*cp*cp*ap*up*gp*up*up*gp*ap*up*gp*gp*up*ap*a)-3') (5 kDa, RNA from Homo sapiens)
- Rna (Complex)
- Efpiwi(n959k)-pirna-16nt target (Complex)
- Efpiwi(n959k) (Complex from Ephydatia fluviatilis)
- Magnesium ion (24 Da, Ligand)
Cryo-EM structure of Hili in complex with piRNA
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 7yfx
Q-score: 0.425
Li Z, Li Z, Zhang Y, Zhou L, Xu Q, Li L, Zeng L, Xue J, Niu H, Zhong J, Yu Q, Li D, Gui M, Huang Y, Tu S, Zhang Z, Song CQ, Wu J, Shen EZ
Nat Struct Mol Biol (2024) [ Pubmed: 38658622 DOI: doi:10.1038/s41594-024-01287-6 ]
- Pirna (Complex)
- Magnesium ion (24 Da, Ligand)
- Pirna (8 kDa, RNA from Homo sapiens)
- Piwi-like protein 2 (114 kDa, Protein from Homo sapiens)
- Hili-pirna (Complex)
- Hili (Complex from Homo sapiens)