Cryo-EM structure of C.crescentus bNY30a pilus complex
Resolution: 0.86 Å
EM Method: Helical reconstruction
Fitted PDBs: 8u2b
Q-score: 0.521
Wang Y, Theodore M, Xing Z, Narsaria U, Yu Z, Zeng L, Zhang J
Sci Adv (2024) 10 pp. eadl4450-eadl4450 [ Pubmed: 38701202 DOI: doi:10.1126/sciadv.adl4450 ]
- Flp family type ivb pilin (4 kDa, Protein from Caulobacter vibrioides)
- Caulobacter crescentus pili (170 kDa, Complex from Caulobacter vibrioides)
PhiCb5 maturation protein with Caulobacter crescentus bNY30a pili
Resolution: 6.45 Å
EM Method: Single-particle
Fitted PDBs: 8ucr
Q-score: 0.158
Wang Y, Theodore M, Xing Z, Narsaria U, Yu Z, Zeng L, Zhang J
Sci Adv (2024) 10 pp. eadl4450-eadl4450 [ Pubmed: 38701202 DOI: doi:10.1126/sciadv.adl4450 ]
- Maturation protein (40 kDa, Protein from Caulobacter phage phiCb5)
- A complex of pilus and phicb5 maturation protein (210 kDa, Complex from Caulobacter vibrioides)
- Flp family type ivb pilin (4 kDa, Protein from Caulobacter vibrioides)
Type IV pilus from Pseudomonas PAO1 strain
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 8tum
Q-score: 0.448
Thongchol J, Yu Z, Harb L, Lin Y, Koch M, Theodore M, Narsaria U, Shaevitz J, Gitai Z, Wu Y, Zhang J, Zeng L
Science (2024) 384 pp. eadl0635-eadl0635 [ Pubmed: 38574145 DOI: doi:10.1126/science.adl0635 ]
- Type iv twitching pilus (240 kDa, Cellular component from Pseudomonas aeruginosa)
- Type iv major pilin protein pila (14 kDa, Protein from Pseudomonas aeruginosa PAO1)
Cryo-EM structure of the EfPiwi (N959K)-piRNA-target ternary complex
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7yfq
Q-score: 0.435
Li Z, Li Z, Zhang Y, Zhou L, Xu Q, Li L, Zeng L, Xue J, Niu H, Zhong J, Yu Q, Li D, Gui M, Huang Y, Tu S, Zhang Z, Song CQ, Wu J, Shen EZ
Nat Struct Mol Biol (2024) [ Pubmed: 38658622 DOI: doi:10.1038/s41594-024-01287-6 ]
- Pirna (7 kDa, RNA from Homo sapiens)
- Piwi (92 kDa, Protein from Ephydatia fluviatilis)
- Rna (5'-r(*up*cp*cp*ap*up*gp*up*up*gp*ap*up*gp*gp*up*ap*a)-3') (5 kDa, RNA from Homo sapiens)
- Rna (Complex)
- Efpiwi(n959k)-pirna-16nt target (Complex)
- Efpiwi(n959k) (Complex from Ephydatia fluviatilis)
- Magnesium ion (24 Da, Ligand)
Cryo-EM structure of Hili in complex with piRNA
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 7yfx
Q-score: 0.425
Li Z, Li Z, Zhang Y, Zhou L, Xu Q, Li L, Zeng L, Xue J, Niu H, Zhong J, Yu Q, Li D, Gui M, Huang Y, Tu S, Zhang Z, Song CQ, Wu J, Shen EZ
Nat Struct Mol Biol (2024) [ Pubmed: 38658622 DOI: doi:10.1038/s41594-024-01287-6 ]
- Pirna (Complex)
- Magnesium ion (24 Da, Ligand)
- Pirna (8 kDa, RNA from Homo sapiens)
- Piwi-like protein 2 (114 kDa, Protein from Homo sapiens)
- Hili-pirna (Complex)
- Hili (Complex from Homo sapiens)
Cryo-EM structure of the Mili-piRNA- target ternary complex
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 7yfy
Q-score: 0.406
Li Z, Li Z, Zhang Y, Zhou L, Xu Q, Li L, Zeng L, Xue J, Niu H, Zhong J, Yu Q, Li D, Gui M, Huang Y, Tu S, Zhang Z, Song CQ, Wu J, Shen EZ
Nat Struct Mol Biol (2024) [ Pubmed: 38658622 DOI: doi:10.1038/s41594-024-01287-6 ]
- Mili (Complex from Mus musculus)
- Magnesium ion (24 Da, Ligand)
- Piwi-like protein 2 (91 kDa, Protein from Mus musculus)
- Rna (Complex)
- Pirna (8 kDa, RNA from Homo sapiens)
- Rna (5'-r(p*cp*cp*ap*up*gp*up*up*gp*ap*up*gp*gp*up*ap*a)-3') (4 kDa, RNA from Homo sapiens)
- Mili-pirna-15nt target (Complex)
Cryo-EM structure of the EfPiwi(N959K) in complex with piRNA
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7yg6
Q-score: 0.399
Li Z, Li Z, Zhang Y, Zhou L, Xu Q, Li L, Zeng L, Xue J, Niu H, Zhong J, Yu Q, Li D, Gui M, Huang Y, Tu S, Zhang Z, Song CQ, Wu J, Shen EZ
Nat Struct Mol Biol (2024) [ Pubmed: 38658622 DOI: doi:10.1038/s41594-024-01287-6 ]
- Pirna (7 kDa, RNA from Homo sapiens)
- Piwi (92 kDa, Protein from Ephydatia fluviatilis)
- Pirna (Complex)
- Efpiwi(n959k) (Complex from Ephydatia fluviatilis)
- Magnesium ion (24 Da, Ligand)
- Efpiwi(n959k)-pirna (Complex)
Cryo-EM structure of the Mili in complex with piRNA
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 7ygn
Q-score: 0.5
Li Z, Li Z, Zhang Y, Zhou L, Xu Q, Li L, Zeng L, Xue J, Niu H, Zhong J, Yu Q, Li D, Gui M, Huang Y, Tu S, Zhang Z, Song CQ, Wu J, Shen EZ
Nat Struct Mol Biol (2024) [ Pubmed: 38658622 DOI: doi:10.1038/s41594-024-01287-6 ]
- Pirna (8 kDa, RNA from Homo sapiens)
- Mili-pirna (Complex)
- Mili (Complex from Mus musculus)
- Pirna (Complex)
- Magnesium ion (24 Da, Ligand)
- Piwi-like protein 2 (91 kDa, Protein from Mus musculus)