E.coli DNA gyrase in complex with 217 bp substrate DNA and LEI-800
Bakker A.T., Kotsogianni I, Avalos M., Punt J.M., Liu B., Piermarini D., Gagestein B., Slingerland C.J., Zhang L., Willemse J.J., Ghimire L.B., van den Berg R.J.H.B.N., Janssen A.P.A., Ottenhoff T.H.M., van Boeckel C.A.A., van Wezel G.P., Ghilarov D., Martin N.I., van der Stelt M.
Nature Chemistry (2024)
Image sets:
E.coli DNA gyrase in complex with 217 bp substrate DNA and LEI-800
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 8qqi
Q-score: 0.553
Bakker AT, Kotsogianni I, Avalos M, Punt JM, Liu B, Piermarini D, Gagestein B, Slingerland CJ, Zhang L, Willemse JJ, Ghimire LB, van den Berg RJHBN, Janssen APA, Ottenhoff THM, van Boeckel CAA, van Wezel GP, Ghilarov D, Martin NI, van der Stelt M
Nat Chem (2024) [ Pubmed: 38898213 DOI: doi:10.1038/s41557-024-01516-x ]
- Mu217 chain e (7 kDa, DNA from Escherichia phage Mu)
- Dna gyrase subunit b (89 kDa, Protein from Escherichia coli)
- Dna gyrase subunit a (58 kDa, Protein from Escherichia coli)
- Ternary complex of e.coli dna gyrase with 217 bp linear dsdna fragment and lei-800 (Complex from Escherichia coli)
- ~{n}-[[(2~{s},5~{r})-5-(4-pyridin-3-ylphenyl)pyrrolidin-2-yl]methyl]isoquinoline-5-sulfonamide (444 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Mu217 chain f (8 kDa, DNA from Escherichia phage Mu)
Cryo-EM structure of CB2-G protein complex
Resolution: 3.08 Å
EM Method: Single-particle
Fitted PDBs: 8guq
Q-score: 0.506
Li X, Chang H, Bouma J, de Paus LV, Mukhopadhyay P, Paloczi J, Mustafa M, van der Horst C, Kumar SS, Wu L, Yu Y, van den Berg RJBHN, Janssen APA, Lichtman A, Liu ZJ, Pacher P, van der Stelt M, Heitman LH, Hua T
Nat Commun (2023) 14 pp. 1447-1447 [ Pubmed: 36922494 DOI: doi:10.1038/s41467-023-37112-9 ]
- Ternary complex of cannabinoid receptor 2 with guanine nucleotide-binding protein and single-chain variable fragment (140 MDa, Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Cannabinoid receptor 2 (39 kDa, Protein from Homo sapiens)
- Olorinab (357 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Scfv16 (27 kDa, Protein from Homo sapiens)
Cryo-EM structure of CP-CB2-G protein complex
Resolution: 2.84 Å
EM Method: Single-particle
Fitted PDBs: 8gur
Q-score: 0.458
Li X, Chang H, Bouma J, de Paus LV, Mukhopadhyay P, Paloczi J, Mustafa M, van der Horst C, Kumar SS, Wu L, Yu Y, van den Berg RJBHN, Janssen APA, Lichtman A, Liu ZJ, Pacher P, van der Stelt M, Heitman LH, Hua T
Nat Commun (2023) 14 pp. 1447-1447 [ Pubmed: 36922494 DOI: doi:10.1038/s41467-023-37112-9 ]
- Ternary complex of cannabinoid receptor 2 with guanine nucleotide-binding protein and single-chain variable fragment (140 MDa, Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Cannabinoid receptor 2 (39 kDa, Protein from Homo sapiens)
- 2-[(1r,2r,5r)-5-hydroxy-2-(3-hydroxypropyl)cyclohexyl]-5-(2-methyloctan-2-yl)phenol (376 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Scfv16 (27 kDa, Protein from Homo sapiens)
Cryo-EM structure of HU-CB2-G protein complex
Resolution: 2.97 Å
EM Method: Single-particle
Fitted PDBs: 8gus
Q-score: 0.458
Li X, Chang H, Bouma J, de Paus LV, Mukhopadhyay P, Paloczi J, Mustafa M, van der Horst C, Kumar SS, Wu L, Yu Y, van den Berg RJBHN, Janssen APA, Lichtman A, Liu ZJ, Pacher P, van der Stelt M, Heitman LH, Hua T
Nat Commun (2023) 14 pp. 1447-1447 [ DOI: doi:10.1038/s41467-023-37112-9 Pubmed: 36922494 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- [(1~{s},4~{s},5~{s})-4-[2,6-dimethoxy-4-(2-methyloctan-2-yl)phenyl]-6,6-dimethyl-2-bicyclo[3.1.1]hept-2-enyl]methanol (414 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Ternary complex of cannabinoid receptor 2 with guanine nucleotide-binding protein and single-chain variable fragment (140 MDa, Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Cannabinoid receptor 2 (39 kDa, Protein from Homo sapiens)
- Scfv16 (27 kDa, Protein from Homo sapiens)
Cryo-EM structure of LEI-CB2-Gi complex
Resolution: 2.98 Å
EM Method: Single-particle
Fitted PDBs: 8gut
Q-score: 0.457
Li X, Chang H, Bouma J, de Paus LV, Mukhopadhyay P, Paloczi J, Mustafa M, van der Horst C, Kumar SS, Wu L, Yu Y, van den Berg RJBHN, Janssen APA, Lichtman A, Liu ZJ, Pacher P, van der Stelt M, Heitman LH, Hua T
Nat Commun (2023) 14 pp. 1447-1447 [ Pubmed: 36922494 DOI: doi:10.1038/s41467-023-37112-9 ]
- Ternary complex of cannabinoid receptor 2 with guanine nucleotide-binding protein and single-chain variable fragment (140 MDa, Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Cannabinoid receptor 2 (37 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein subunit gamma (7 kDa, Protein from Homo sapiens)
- 1-[[4-[5-fluoranyl-6-[(oxan-4-ylamino)methyl]pyridin-2-yl]phenyl]methyl]-3-(2-methylpropyl)imidazolidine-2,4-dione (454 Da, Ligand)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Scfv16 (27 kDa, Protein from Homo sapiens)