Dam KA, Barnes CO, Gristick HB, Schoofs T, Gnanapragasam PNP, Nussenzweig MC, Bjorkman PJ
Nat Commun (2022) 13 pp. 6123-6123 [ Pubmed: 36253376 DOI: doi:10.1038/s41467-022-33860-2 ]
- 10-1074 fab heavy chain (14 kDa, Protein from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Bg24 mature fab heavy chain (13 kDa, Protein from Homo sapiens)
- 10-1074 fab light chain (11 kDa, Protein from Homo sapiens)
- Cryo-em map of bg24 fabs with an inferred germline light chain and 10-1074 fabs in complex with hiv-1 env immunogen bg505-sosipv4.1-gt1 containing the n276 gp120 glycan- class 1 (Complex from Human immunodeficiency virus 1)
- Envelope glycoprotein gp120 (49 kDa, Protein from Human immunodeficiency virus 1)
- Envelope glycoprotein gp41 (14 kDa, Protein from Human immunodeficiency virus 1)
- Bg24 mature fab light chain (11 kDa, Protein from Homo sapiens)
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 7ugq
Q-score: 0.47
Dam KA, Barnes CO, Gristick HB, Schoofs T, Gnanapragasam PNP, Nussenzweig MC, Bjorkman PJ
Nat Commun (2022) 13 pp. 6123-6123 [ DOI: doi:10.1038/s41467-022-33860-2 Pubmed: 36253376 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Bg24 with an inferred germline cdrl1 fab heavy chain (13 kDa, Protein from Homo sapiens)
- Bg24 with an inferred germline cdrl1 fab light chain (11 kDa, Protein from Homo sapiens)
- Envelope glycoprotein gp41 (14 kDa, Protein from Human immunodeficiency virus 1)
- 10-1074 fab heavy chain (14 kDa, Protein from Homo sapiens)
- Envelope glycoprotein gp120 (50 kDa, Protein from Human immunodeficiency virus 1)
- 10-1074 fab light chain (11 kDa, Protein from Homo sapiens)
- Cryo-em map of bg24 fabs with an inferred germline cdrl1 and 10-1074 fabs in complex with hiv-1 env 6405-sosip.664 (Complex from Human immunodeficiency virus 1)
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 7ugn
Q-score: 0.477
Dam KA, Barnes CO, Gristick HB, Schoofs T, Gnanapragasam PNP, Nussenzweig MC, Bjorkman PJ
Nat Commun (2022) 13 pp. 6123-6123 [ DOI: doi:10.1038/s41467-022-33860-2 Pubmed: 36253376 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Envelope glycoprotein gp41 (14 kDa, Protein from Human immunodeficiency virus 1)
- 10-1074 fab heavy chain (14 kDa, Protein from Homo sapiens)
- Cryo-em map of bg24-igl cdr3igl and 10-1074 fabs in complex with bg505 sosip.664v4.1-gt1 env trimer immunogen (Complex from Human immunodeficiency virus 1)
- Bg24 inferred germline fab with germline cdr3s light chain (11 kDa, Protein from Homo sapiens)
- 10-1074 fab light chain (11 kDa, Protein from Homo sapiens)
- Bg24 inferred germline fab with germline cdr3s heavy chain (13 kDa, Protein from Homo sapiens)
- Envelope glycoprotein gp120 (50 kDa, Protein from Human immunodeficiency virus 1)
- Alpha-d-mannopyranose (180 Da, Ligand)
Resolution: 4.1 Å
EM Method: Single-particle
Fitted PDBs: 7ugo
Q-score: 0.425
Dam KA, Barnes CO, Gristick HB, Schoofs T, Gnanapragasam PNP, Nussenzweig MC, Bjorkman PJ
Nat Commun (2022) 13 pp. 6123-6123 [ DOI: doi:10.1038/s41467-022-33860-2 Pubmed: 36253376 ]
- Envelope glycoprotein gp120 (52 kDa, Protein from Human immunodeficiency virus 1)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- 10-1074 fab light chain (11 kDa, Protein from Homo sapiens)
- 10-1074 fab heavy chain (14 kDa, Protein from Homo sapiens)
- Envelope glycoprotein gp41 (14 kDa, Protein from Human immunodeficiency virus 1)
- Bg24 inferred germline fab with mature cdr3s heavy chain (13 kDa, Protein from Homo sapiens)
- Bg24 inferred germline fab with mature cdr3s light chain (11 kDa, Protein from Homo sapiens)
- Cryo-em map of bg24-igl cdr3mat and 10-1074 fabs in complex with bg505 sosip.664v4.1-gt1 env trimer immunogen (Complex from Human immunodeficiency virus 1)
- Alpha-d-mannopyranose (180 Da, Ligand)
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 7ucg
Q-score: 0.411
Barnes CO, Schoofs T, Gnanapragasam PNP, Golijanin J, Huey-Tubman KE, Gruell H, Schommers P, Suh-Toma N, Lee YE, Cetrulo Lorenzi JC, Piechocka-Trocha A, Scheid JF, West Jr AP, Walker BD, Seaman MS, Klein F, Nussenzweig MC, Bjorkman PJ
Sci Adv (2022) 8 pp. eabp8155-eabp8155 [ Pubmed: 35960796 DOI: doi:10.1126/sciadv.abp8155 ]
- Complex of du422 sosip trimer bound to 10-1074 and bg24 fab fragments (650 kDa, Complex from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- 10-1074 fab heavy chain (26 kDa, Protein from Homo sapiens)
- Bg24 light chain (21 kDa, Protein from Homo sapiens)
- Bg24 cdrh2-v2 fab heavy chain (25 kDa, Protein from Homo sapiens)
- 10-1074 light chain (23 kDa, Protein from Homo sapiens)
- Envelope glycoprotein gp41 (17 kDa, Protein from Human immunodeficiency virus 1)
- Envelope glycoprotein gp160 (56 kDa, Protein from Human immunodeficiency virus 1)
VRC03 and 10-1074 Bound BG505 F14 HIV-1 SOSIP Envelope Trimer Structure
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 6v8z
Q-score: 0.358
Henderson R, Lu M, Zhou Y, Mu Z, Parks R, Han Q, Hsu AL, Carter E, Blanchard SC, Edwards RJ, Wiehe K, Saunders KO, Borgnia MJ, Bartesaghi A, Mothes W, Haynes BF, Acharya P, Munir Alam S
Nat Commun (2020) 11 pp. 520-520 [ Pubmed: 31980614 DOI: doi:10.1038/s41467-019-14196-w ]
- 10-1074 fab heavy chain (25 kDa, Protein from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Vrc03 fab light chain (23 kDa, Protein from Homo sapiens)
- Envelope glycoprotein gp120 (52 kDa, Protein from Human immunodeficiency virus 1)
- Bg505 f14/vt8 hiv-1 sosip env trimer (Complex from Human immunodeficiency virus 1)
- 10-1074 fab light chain (22 kDa, Protein from Homo sapiens)
- Envelope glycoprotein gp41 (16 kDa, Protein from Human immunodeficiency virus 1)
- Vrc03 fab heavy chain (25 kDa, Protein from Homo sapiens)
- 10-1074 (Complex from Homo sapiens)
- Vrc03 (Complex from Homo sapiens)
- Complex between vrc03, 10-1074, and bg505 f14 hiv-1 sosip env trimer (Complex)
HIV-1 bNAb 1-18 in complex with BG505 SOSIP.664 and 10-1074
Resolution: 2.5 Å
EM Method: Single-particle
Fitted PDBs: 6udj
Q-score: 0.632
Schommers P, Gruell H, Abernathy ME, Tran MK, Dingens AS, Gristick HB, Barnes CO, Schoofs T, Schlotz M, Vanshylla K, Kreer C, Weiland D, Holtick U, Scheid C, Valter MM, van Gils MJ, Sanders RW, Vehreschild JJ, Cornely OA, Lehmann C, Fatkenheuer G, Seaman MS, Bloom JD, Bjorkman PJ, Klein F
Cell (2020) 180 pp. 471- [ DOI: doi:10.1016/j.cell.2020.01.010 Pubmed: 32004464 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- 1-18 fab (Complex from Homo sapiens)
- Bg505 sosip.664 trimer (Complex from Human immunodeficiency virus 1)
- 10-1074 fab light chain (23 kDa, Protein from Homo sapiens)
- 10-1074 fab heavy chain (25 kDa, Protein from Homo sapiens)
- Envelope glycoprotein gp41 (17 kDa, Protein from Human immunodeficiency virus 1)
- 1-18 fab light chain (25 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Envelope glycoprotein gp120 (53 kDa, Protein from Human immunodeficiency virus 1)
- Complex of bnabs 1-18 and 10-1074 in complex with bg505 sosip.664 trimer (300 kDa, Complex)
- 1-18 fab heavy chain (53 kDa, Protein from Homo sapiens)
- 10-1074 fab (Complex from Homo sapiens)
HIV-1 bNAb 1-55 in complex with modified BG505 SOSIP-based immunogen RC1 and 10-1074
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 6udk
Q-score: 0.442
Schommers P, Gruell H, Abernathy ME, Tran MK, Dingens AS, Gristick HB, Barnes CO, Schoofs T, Schlotz M, Vanshylla K, Kreer C, Weiland D, Holtick U, Scheid C, Valter MM, van Gils MJ, Sanders RW, Vehreschild JJ, Cornely OA, Lehmann C, Fatkenheuer G, Seaman MS, Bloom JD, Bjorkman PJ, Klein F
Cell (2020) 180 pp. 471- [ DOI: doi:10.1016/j.cell.2020.01.010 Pubmed: 32004464 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Complex of three bnab 1-55 fabs and three 10-1074 fabs bound to rc1 variant of bg505 sosip.664 trimer (300 kDa, Complex)
- 10-1074 fab light chain (23 kDa, Protein from Homo sapiens)
- 10-1074 fab heavy chain (25 kDa, Protein from Homo sapiens)
- 1-55 fab heavy chain (28 kDa, Protein from Homo sapiens)
- 1-55 fab light chain (25 kDa, Protein from Homo sapiens)
- Rc1 variant of hiv-1 bg505 sosip.664 (Complex from Human immunodeficiency virus 1)
- Rc1 variant of hiv-1 env glycoprotein gp120 (54 kDa, Protein from Human immunodeficiency virus 1)
- 10-1074 fab (Complex from Homo sapiens)
- 1-55 fab (Complex from Homo sapiens)
- Rc1 variant of hiv-1 env glycoprotein gp41 (17 kDa, Protein from Human immunodeficiency virus 1)