Cryo-EM structure of DiCas7-11 in complex with crRNA
Resolution: 2.93 Å
EM Method: Single-particle
Fitted PDBs: 8wm4
Q-score: 0.435
Hong T, Luo Q, Ma H, Wang X, Li X, Shen C, Pang J, Wang Y, Chen Y, Zhang C, Su Z, Dong H, Tang X
Signal Transduct Target Ther (2024) 9 pp. 111-111 [ Pubmed: 38735995 DOI: doi:10.1038/s41392-024-01821-4 ]
- Binary complex of cas7-11 and crrna (Complex from Escherichia coli)
- Zinc ion (65 Da, Ligand)
- Crispr-associated ramp family protein (183 kDa, Protein from Desulfonema ishimotonii)
- Crrna (38-mer) (12 kDa, RNA from Escherichia coli)
Cryo-EM structure of DiCas7-11-crRNA in complex with regulator
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 8wmc
Q-score: 0.538
Hong T, Luo Q, Ma H, Wang X, Li X, Shen C, Pang J, Wang Y, Chen Y, Zhang C, Su Z, Dong H, Tang X
Signal Transduct Target Ther (2024) 9 pp. 111-111 [ Pubmed: 38735995 DOI: doi:10.1038/s41392-024-01821-4 ]
- Ternary complex of cas7-11_crrna_tpr-chat (Complex from Escherichia coli)
- Chat domain-containing protein (88 kDa, Protein from Desulfonema ishimotonii)
- Crispr-associated ramp family protein (183 kDa, Protein from Desulfonema ishimotonii)
- Zinc ion (65 Da, Ligand)
- Rna (38-mer) (12 kDa, RNA from Escherichia coli)
Cryo-EM structure of DiCas7-11 mutant in complex with crRNA
Resolution: 3.53 Å
EM Method: Single-particle
Fitted PDBs: 8wmi
Q-score: 0.426
Hong T, Luo Q, Ma H, Wang X, Li X, Shen C, Pang J, Wang Y, Chen Y, Zhang C, Su Z, Dong H, Tang X
Signal Transduct Target Ther (2024) 9 pp. 111-111 [ Pubmed: 38735995 DOI: doi:10.1038/s41392-024-01821-4 ]
- Zinc ion (65 Da, Ligand)
- Crrna (39-mer) (12 kDa, RNA from Escherichia coli)
- Crispr-associated ramp family protein (183 kDa, Protein from Desulfonema ishimotonii)
- Binary complex of dicas7-11 mutant with crrna (Complex from Escherichia coli)
Cryo-EM structure of Cas7-11-crRNA bound to N-terminal of TPR-CHAT
Resolution: 2.86 Å
EM Method: Single-particle
Fitted PDBs: 8wml
Q-score: 0.522
Hong T, Luo Q, Ma H, Wang X, Li X, Shen C, Pang J, Wang Y, Chen Y, Zhang C, Su Z, Dong H, Tang X
Signal Transduct Target Ther (2024) 9 pp. 111-111 [ Pubmed: 38735995 DOI: doi:10.1038/s41392-024-01821-4 ]
- Chat domain-containing protein (7 kDa, Protein from Desulfonema ishimotonii)
- Crispr-associated ramp family protein (183 kDa, Protein from Desulfonema ishimotonii)
- Ternary complex of cas7-11-crrna bound to n-terminal of tpr-chat (Complex from Escherichia coli)
- Zinc ion (65 Da, Ligand)
- Crrna (38-mer) (12 kDa, RNA from Escherichia coli)
Cryo-EM structure of Cas7-11-crRNA binary complex
Resolution: 3.53 Å
EM Method: Single-particle
Fitted PDBs: 7yn9
Q-score: 0.51
Huo Y, Zhao H, Dong Q, Jiang T
Nat Microbiol (2023) 8 pp. 522-532 [ Pubmed: 36702942 DOI: doi:10.1038/s41564-022-01316-4 ]
- Crrna (15 kDa, RNA from Desulfonema ishimotonii)
- Cas7-11-crrna binary complex (Complex from Desulfonema ishimotonii)
- Crispr-associated ramp family protein (183 kDa, Protein from Desulfonema ishimotonii)
Cryo-EM structure of Cas7-11-crRNA bound to target RNA-1
Resolution: 3.64 Å
EM Method: Single-particle
Fitted PDBs: 7yna
Q-score: 0.501
Huo Y, Zhao H, Dong Q, Jiang T
Nat Microbiol (2023) 8 pp. 522-532 [ Pubmed: 36702942 DOI: doi:10.1038/s41564-022-01316-4 ]
- Crrna (38-mer) (15 kDa, RNA from Desulfonema ishimotonii)
- Target rna-1 (25-mer) (10 kDa, RNA from Desulfonema ishimotonii)
- The complex of cas7-11-crrna bound to target rna-1 (Complex from Desulfonema ishimotonii)
- Crispr-associated ramp family protein (183 kDa, Protein from Desulfonema ishimotonii)
Cryo-EM structure of Cas7-11-crRNA bound to target RNA-2
Resolution: 3.46 Å
EM Method: Single-particle
Fitted PDBs: 7ynb
Q-score: 0.512
Huo Y, Zhao H, Dong Q, Jiang T
Nat Microbiol (2023) 8 pp. 522-532 [ Pubmed: 36702942 DOI: doi:10.1038/s41564-022-01316-4 ]
- The complex of cas7-11-crrna bound to target rna-2 (Complex from Desulfonema ishimotonii)
- Target rna-2 (28-mer) (14 kDa, RNA from Desulfonema ishimotonii)
- Crrna (38-mer) (15 kDa, RNA from Desulfonema ishimotonii)
- Crispr-associated ramp family protein (183 kDa, Protein from Desulfonema ishimotonii)
Cryo-EM structure of Cas7-11-crRNA bound to target RNA-3
Resolution: 3.14 Å
EM Method: Single-particle
Fitted PDBs: 7ync
Q-score: 0.542
Huo Y, Zhao H, Dong Q, Jiang T
Nat Microbiol (2023) 8 pp. 522-532 [ Pubmed: 36702942 DOI: doi:10.1038/s41564-022-01316-4 ]
- Target rna-3 (28-mer) (15 kDa, RNA from Desulfonema ishimotonii)
- The complex of cas7-11-crrna bound to target rna-3 (Complex from Desulfonema ishimotonii)
- Crrna (38-mer) (15 kDa, RNA from Desulfonema ishimotonii)
- Crispr-associated ramp family protein (183 kDa, Protein from Desulfonema ishimotonii)
Cryo-EM structure of Cas7-11-crRNA-Csx29 ternary complex
Resolution: 3.29 Å
EM Method: Single-particle
Fitted PDBs: 7ynd
Q-score: 0.526
Huo Y, Zhao H, Dong Q, Jiang T
Nat Microbiol (2023) 8 pp. 522-532 [ Pubmed: 36702942 DOI: doi:10.1038/s41564-022-01316-4 ]
- Cas7-11-crrna-csx29 ternary complex (Complex from Desulfonema ishimotonii)
- Crrna (38-mer) (15 kDa, RNA from Desulfonema ishimotonii)
- Crispr-associated ramp family protein (183 kDa, Protein from Desulfonema ishimotonii)
- Chat domain-containing protein (88 kDa, Protein from Desulfonema ishimotonii)
Structure of the Cas7-11-Csx29-guide RNA complex
Resolution: 2.49 Å
EM Method: Single-particle
Fitted PDBs: 7y9x
Q-score: 0.59
Kato K, Okazaki S, Schmitt-Ulms C, Jiang K, Zhou W, Ishikawa J, Isayama Y, Adachi S, Nishizawa T, Makarova KS, Koonin EV, Abudayyeh OO, Gootenberg JS, Nishimasu H
Science (2022) 378 pp. 882-889 [ Pubmed: 36423304 DOI: doi:10.1126/science.add7347 ]
- Cas7-11 in complex with a crrna and its target rna (Complex from Desulfonema ishimotonii)
- Crispr-associated ramp family protein (185 kDa, Protein from Desulfonema ishimotonii)
- Zinc ion (65 Da, Ligand)
- Rna (Complex)
- Cas7-11, csx29 (Complex from Desulfonema ishimotonii)
- Chat domain-containing protein (88 kDa, Protein from Desulfonema ishimotonii)
- Crrna (12 kDa, RNA from Desulfonema ishimotonii)