Human spliceosomal PM5 C* complex
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 8c6j
Q-score: 0.353
Dybkov O, Preussner M, El Ayoubi L, Feng VY, Harnisch C, Merz K, Leupold P, Yudichev P, Agafonov DE, Will CL, Girard C, Dienemann C, Urlaub H, Kastner B, Heyd F, Luehrmann R
Sci Adv (2023) 9 pp. eadf1785- [ DOI: doi:10.1126/sciadv.adf1785 Pubmed: 36867703 ]
- Peptidyl-prolyl cis-trans isomerase-like 3 (18 kDa, Protein from human)
- U5 snrna (36 kDa, RNA from human)
- Crooked neck-like protein 1 (83 kDa, Protein from human)
- Small nuclear ribonucleoprotein g (8 kDa, Protein from human)
- Small nuclear ribonucleoprotein sm d1 (13 kDa, Protein from human)
- Rna-binding protein 8a (19 kDa, Protein from human)
- Pre-mrna-splicing factor isy1 homolog (33 kDa, Protein from human)
- Pre-mrna-processing factor 17 (65 kDa, Protein from human)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Intron-binding protein aquarius (171 kDa, Protein from human)
- Pre-mrna-processing factor 19 (55 kDa, Protein from human)
- Protein mago nashi homolog (17 kDa, Protein from human)
- Small nuclear ribonucleoprotein f (9 kDa, Protein from human)
- Inositol hexakisphosphate (660 Da, Ligand)
- U6 snrna (34 kDa, RNA from human)
- U5 small nuclear ribonucleoprotein 200 kda helicase (244 kDa, Protein from human)
- Nitric oxide synthase-interacting protein (33 kDa, Protein from human)
- Pm5 5' exon (78 kDa, RNA from Homo sapiens)
- Small nuclear ribonucleoprotein sm d2 (13 kDa, Protein from human)
- Pleiotropic regulator 1 (57 kDa, Protein from human)
- Peptidyl-prolyl cis-trans isomerase-like 1 (18 kDa, Protein from human)
- U2 snrna (60 kDa, RNA from human)
- Small nuclear ribonucleoprotein sm d3 (13 kDa, Protein from human)
- Potassium ion (39 Da, Ligand)
- Pre-mrna-splicing factor cwc22 homolog (57 kDa, Protein from human)
- Zinc ion (65 Da, Ligand)
- Atp-dependent rna helicase dhx8 (139 kDa, Protein from human)
- Splicing factor cactin (88 kDa, Protein from human)
- Splicing regulator sde2 (49 kDa, Protein from human)
- Pre-mrna-splicing factor slu7 (68 kDa, Protein from human)
- U2 small nuclear ribonucleoprotein b'' (25 kDa, Protein from human)
- Cell division cycle 5-like protein (92 kDa, Protein from human)
- Prkr-interacting protein 1 (21 kDa, Protein from human)
- Pre-mrna-splicing factor spf27 (26 kDa, Protein from human)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Human spliceosomal c* complex assembled on pm5 pre-mrna (Complex from Homo sapiens)
- Spliceosome-associated protein cwc15 homolog (26 kDa, Protein from human)
- Sting er exit protein (25 kDa, Protein from human)
- Protein fam32a (13 kDa, Protein from human)
- Pre-mrna-splicing factor rbm22 (46 kDa, Protein from human)
- Magnesium ion (24 Da, Ligand)
- U5 small nuclear ribonucleoprotein 40 kda protein (39 kDa, Protein from human)
- Small nuclear ribonucleoprotein e (10 kDa, Protein from human)
- Probable atp-dependent rna helicase ddx41 (69 kDa, Protein from human)
- Pre-mrna-splicing factor syf1 (100 kDa, Protein from human)
- 116 kda u5 small nuclear ribonucleoprotein component (109 kDa, Protein from human)
- Snw domain-containing protein 1 (61 kDa, Protein from human)
- Protein bud31 homolog (17 kDa, Protein from human)
- Nf-kappa-b-activating protein (47 kDa, Protein from human)
- Peptidyl-prolyl cis-trans isomerase e (33 kDa, Protein from human)
- U2 small nuclear ribonucleoprotein a' (28 kDa, Protein from human)
- Small nuclear ribonucleoprotein-associated proteins b and b' (24 kDa, Protein from human)
- Eukaryotic initiation factor 4a-iii (46 kDa, Protein from human)
- Splicing factor c9orf78 (33 kDa, Protein from human)
- Protein fam50a (40 kDa, Protein from human)
- Pm5 intron (74 kDa, RNA from Homo sapiens)
- Pre-mrna-splicing factor syf2 (28 kDa, Protein from human)
- Serine/arginine repetitive matrix protein 2 (300 kDa, Protein from human)
- Pre-mrna-processing-splicing factor 8 (273 kDa, Protein from human)
- Splicing factor ess-2 homolog (52 kDa, Protein from human)
Structural organization of a late activated human spliceosome (Baqr, core region)
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 7qtt
Q-score: 0.384
Schmitzova J, Cretu C, Dienemann C, Urlaub H, Pena V
Nature (2023) 617 pp. 842-850 [ DOI: doi:10.1038/s41586-023-06049-w Pubmed: 37165190 ]
- Putative pre-mrna-splicing factor atp-dependent rna helicase dhx16 (119 kDa, Protein from human)
- Pre-mrna-splicing factor cwc22 homolog (105 kDa, Protein from human)
- U2snrna (60 kDa, RNA from human)
- Crooked neck-like protein 1 (100 kDa, Protein from human)
- Small nuclear ribonucleoprotein g (8 kDa, Protein from human)
- Small nuclear ribonucleoprotein sm d1 (13 kDa, Protein from human)
- Phd finger-like domain-containing protein 5a (12 kDa, Protein from human)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Inositol hexakisphosphate (660 Da, Ligand)
- Small nuclear ribonucleoprotein f (9 kDa, Protein from human)
- Small nuclear ribonucleoprotein sm d2 (13 kDa, Protein from human)
- Pleiotropic regulator 1 (57 kDa, Protein from human)
- Snw domain-containing protein 1 (61 kDa, Protein from human)
- Peptidyl-prolyl cis-trans isomerase-like 1 (18 kDa, Protein from human)
- Late human activated spliceosome arrested with a dominant-negative mutant of the splicing helicase aquarius (aquarius k829a) (Complex from Homo sapiens)
- Small nuclear ribonucleoprotein sm d3 (13 kDa, Protein from human)
- Splicing factor 3a subunit 2 (49 kDa, Protein from human)
- Splicing factor 3b subunit 1 (146 kDa, Protein from human)
- Bud13 homolog (70 kDa, Protein from human)
- Zinc ion (65 Da, Ligand)
- Ring-type e3 ubiquitin-protein ligase ppil2 (58 kDa, Protein from human)
- G-patch domain and kow motifs-containing protein (52 kDa, Protein from human)
- Peptidyl-prolyl cis-trans isomerase-like 4 (57 kDa, Protein from human)
- Cell division cycle 5-like protein (92 kDa, Protein from human)
- Splicing factor 3b subunit 2 (100 kDa, Protein from human)
- Minx (102 kDa, RNA from unidentified adenovirus)
- Splicing factor 3b subunit 3 (135 kDa, Protein from human)
- U6snrna (34 kDa, RNA from Homo sapiens)
- Spliceosome-associated protein cwc15 homolog (26 kDa, Protein from human)
- Pre-mrna-splicing factor rbm22 (46 kDa, Protein from human)
- Magnesium ion (24 Da, Ligand)
- Splicing factor 3b subunit 4 (44 kDa, Protein from human)
- Small nuclear ribonucleoprotein e (10 kDa, Protein from human)
- 116 kda u5 small nuclear ribonucleoprotein component (109 kDa, Protein from human)
- Protein bud31 homolog (17 kDa, Protein from human)
- Ring finger protein 113a (38 kDa, Protein from human)
- Small nuclear ribonucleoprotein-associated proteins b and b' (24 kDa, Protein from human)
- Splicing factor 3b subunit 5 (10 kDa, Protein from human)
- Splicing factor 3a subunit 3 (58 kDa, Protein from human)
- Serine/arginine repetitive matrix protein 2 (300 kDa, Protein from human)
- Pre-mrna-processing-splicing factor 8 (273 kDa, Protein from human)
- U5 snrna (37 kDa, RNA from human)
Resolution: 5.9 Å
EM Method: Single-particle
Fitted PDBs: 8ch6
Q-score: 0.154
Schmitzova J, Cretu C, Dienemann C, Urlaub H, Pena V
Nature (2023) 617 pp. 842-850 [ DOI: doi:10.1038/s41586-023-06049-w Pubmed: 37165190 ]
- U2 small nuclear ribonucleoprotein a' (28 kDa, Protein from human)
- Rnu5a-1 (37 kDa, RNA from human)
- Pre-mrna-processing-splicing factor 8 (273 kDa, Protein from human)
- Ring finger protein 113a (38 kDa, Protein from human)
- Small nuclear ribonucleoprotein e (10 kDa, Protein from human)
- Pre-mrna-processing factor 17 (65 kDa, Protein from human)
- Snw domain-containing protein 1 (61 kDa, Protein from human)
- Splicing factor 3b subunit 5 (10 kDa, Protein from human)
- Cell division cycle 5-like protein (92 kDa, Protein from human)
- U5 small nuclear ribonucleoprotein 200 kda helicase (244 kDa, Protein from human)
- Small nuclear ribonucleoprotein sm d2 (13 kDa, Protein from human)
- Pre-mrna-splicing factor cwc22 homolog (105 kDa, Protein from human)
- Rnu2-1 (60 kDa, RNA from human)
- Pre-mrna-splicing factor syf1 (100 kDa, Protein from human)
- Pre-mrna-splicing factor spf27 (26 kDa, Protein from human)
- Peptidyl-prolyl cis-trans isomerase e (33 kDa, Protein from human)
- Inositol hexakisphosphate (660 Da, Ligand)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Pre-mrna-splicing factor rbm22 (46 kDa, Protein from human)
- Peptidyl-prolyl cis-trans isomerase-like 4 (57 kDa, Protein from human)
- Splicing factor 3b subunit 4 (44 kDa, Protein from human)
- Magnesium ion (24 Da, Ligand)
- Splicing factor 3b subunit 1 (146 kDa, Protein from human)
- Crooked neck-like protein 1 (100 kDa, Protein from human)
- Late human activated spliceosome arrested with a dominant-negative mutant of the splicing helicase aquarius (aquarius k829a) (Complex from Homo sapiens)
- Intron-binding protein aquarius (171 kDa, Protein from human)
- G-patch domain and kow motifs-containing protein (52 kDa, Protein from human)
- Small nuclear ribonucleoprotein sm d1 (13 kDa, Protein from human)
- Splicing factor 3a subunit 3 (58 kDa, Protein from human)
- Rnu6-1 (34 kDa, RNA from Homo sapiens)
- Pre-mrna-splicing factor isy1 homolog (33 kDa, Protein from human)
- Phd finger-like domain-containing protein 5a (12 kDa, Protein from human)
- Splicing factor 3a subunit 1 (88 kDa, Protein from human)
- Pre-mrna-processing factor 19 (55 kDa, Protein from human)
- Minx-m3 (102 kDa, RNA from unidentified adenovirus)
- Small nuclear ribonucleoprotein sm d3 (13 kDa, Protein from human)
- Peptidyl-prolyl cis-trans isomerase-like 1 (18 kDa, Protein from human)
- U5 small nuclear ribonucleoprotein 40 kda protein (39 kDa, Protein from human)
- Splicing factor 3b subunit 3 (135 kDa, Protein from human)
- Zinc ion (65 Da, Ligand)
- Small nuclear ribonucleoprotein f (9 kDa, Protein from human)
- Spliceosome-associated protein cwc15 homolog (26 kDa, Protein from human)
- Putative pre-mrna-splicing factor atp-dependent rna helicase dhx16 (119 kDa, Protein from human)
- Ring-type e3 ubiquitin-protein ligase ppil2 (58 kDa, Protein from human)
- Small nuclear ribonucleoprotein-associated proteins b and b' (24 kDa, Protein from human)
- Pleiotropic regulator 1 (57 kDa, Protein from human)
- Bud13 homolog (70 kDa, Protein from human)
- 116 kda u5 small nuclear ribonucleoprotein component (109 kDa, Protein from human)
- Protein bud31 homolog (17 kDa, Protein from human)
- Serine/arginine repetitive matrix protein 2 (300 kDa, Protein from human)
- Splicing factor 3b subunit 2 (100 kDa, Protein from human)
- U2 small nuclear ribonucleoprotein b'' (25 kDa, Protein from human)
- Splicing factor 3a subunit 2 (49 kDa, Protein from human)
- Small nuclear ribonucleoprotein g (8 kDa, Protein from human)
The cryo-EM structure of human pre-C*-I complex
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 7w59
Q-score: 0.268
Zhan X, Lu Y, Zhang X, Yan C, Shi Y
Mol Cell (2022) 82 pp. 2769-2778.e4 [ DOI: doi:10.1016/j.molcel.2022.05.021 Pubmed: 35705093 ]
- U2 small nuclear ribonucleoprotein a' (28 kDa, Protein from human)
- Atp-dependent rna helicase dhx8 (139 kDa, Protein from human)
- Pre-mrna-processing-splicing factor 8 (273 kDa, Protein from human)
- Pre-mrna-processing factor 17 (65 kDa, Protein from human)
- Small nuclear ribonucleoprotein e (10 kDa, Protein from human)
- Cell division cycle 5-like protein (92 kDa, Protein from human)
- Pre-mrna-splicing factor cwc22 homolog (105 kDa, Protein from human)
- U6 snrna (34 kDa, RNA from Human)
- Small nuclear ribonucleoprotein sm d2 (13 kDa, Protein from human)
- Pre-mrna-splicing factor syf1 (100 kDa, Protein from human)
- U2 small nuclear ribonucleoprotein b'' (25 kDa, Protein from human)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Pre-mrna-splicing factor spf27 (26 kDa, Protein from human)
- Peptidyl-prolyl cis-trans isomerase e (33 kDa, Protein from human)
- Inositol hexakisphosphate (660 Da, Ligand)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Pre-mrna-splicing factor rbm22 (46 kDa, Protein from human)
- Magnesium ion (24 Da, Ligand)
- Crooked neck-like protein 1 (100 kDa, Protein from human)
- Small nuclear ribonucleoprotein sm d1 (13 kDa, Protein from human)
- Psme3-interacting protein (28 kDa, Protein from human)
- Protein mago nashi homolog (17 kDa, Protein from human)
- Rna-binding protein 8a (19 kDa, Protein from human)
- Peptidyl-prolyl cis-trans isomerase-like 1 (18 kDa, Protein from human)
- Human pre-c*-i complex (Complex from Homo sapiens)
- Small nuclear ribonucleoprotein sm d3 (13 kDa, Protein from human)
- Pre-mrna-processing factor 19 (55 kDa, Protein from human)
- Snw domain-containing protein 1 (61 kDa, Protein from human)
- Pre-mrna (55 kDa, RNA from unidentified adenovirus)
- U5 small nuclear ribonucleoprotein 40 kda protein (39 kDa, Protein from human)
- Rna helicase aquarius (171 kDa, Protein from human)
- Eukaryotic initiation factor 4a-iii (46 kDa, Protein from human)
- U5 snrna (37 kDa, RNA from human)
- Pre-mrna (14 kDa, RNA from unidentified adenovirus)
- Zinc ion (65 Da, Ligand)
- Small nuclear ribonucleoprotein f (9 kDa, Protein from human)
- U2 snrna (60 kDa, RNA from human)
- Small nuclear ribonucleoprotein-associated proteins b and b' (24 kDa, Protein from human)
- Pleiotropic regulator 1 (57 kDa, Protein from human)
- 116 kda u5 small nuclear ribonucleoprotein component (109 kDa, Protein from human)
- Protein bud31 homolog (17 kDa, Protein from human)
- Small nuclear ribonucleoprotein g (8 kDa, Protein from human)
- Serine/arginine repetitive matrix protein 2 (300 kDa, Protein from human)
- Pre-mrna-splicing factor syf2 (28 kDa, Protein from human)
- Protein casc3 (76 kDa, Protein from human)
- Spliceosome-associated protein cwc15 homolog (26 kDa, Protein from human)
The cryo-EM structure of human pre-C*-II complex
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 7w5a
Q-score: 0.269
Zhan X, Lu Y, Zhang X, Yan C, Shi Y
Mol Cell (2022) 82 pp. 2769-2778.e4 [ DOI: doi:10.1016/j.molcel.2022.05.021 Pubmed: 35705093 ]
- U2 small nuclear ribonucleoprotein a' (28 kDa, Protein from human)
- Atp-dependent rna helicase dhx8 (139 kDa, Protein from human)
- Pre-mrna-splicing factor slu7 (68 kDa, Protein from human)
- Pre-mrna-processing-splicing factor 8 (273 kDa, Protein from human)
- Cactin (88 kDa, Protein from human)
- Pre-mrna-processing factor 17 (65 kDa, Protein from human)
- Small nuclear ribonucleoprotein e (10 kDa, Protein from human)
- Cell division cycle 5-like protein (92 kDa, Protein from human)
- Pre-mrna-splicing factor cwc22 homolog (105 kDa, Protein from human)
- Small nuclear ribonucleoprotein sm d2 (13 kDa, Protein from human)
- Pre-mrna (55 kDa, RNA from unidentified adenovirus)
- Human pre-c*-ii complex (Complex from Homo sapiens)
- Pre-mrna-splicing factor syf1 (100 kDa, Protein from human)
- U2 small nuclear ribonucleoprotein b'' (25 kDa, Protein from human)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Pre-mrna-splicing factor spf27 (26 kDa, Protein from human)
- Peptidyl-prolyl cis-trans isomerase e (33 kDa, Protein from human)
- Inositol hexakisphosphate (660 Da, Ligand)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Pre-mrna-splicing factor rbm22 (46 kDa, Protein from human)
- Magnesium ion (24 Da, Ligand)
- Crooked neck-like protein 1 (100 kDa, Protein from human)
- Small nuclear ribonucleoprotein sm d1 (13 kDa, Protein from human)
- Psme3-interacting protein (28 kDa, Protein from human)
- Protein mago nashi homolog (17 kDa, Protein from human)
- Rna-binding protein 8a (19 kDa, Protein from human)
- Peptidyl-prolyl cis-trans isomerase-like 1 (18 kDa, Protein from human)
- Small nuclear ribonucleoprotein sm d3 (13 kDa, Protein from human)
- Pre-mrna-processing factor 19 (55 kDa, Protein from human)
- Snw domain-containing protein 1 (61 kDa, Protein from human)
- U5 small nuclear ribonucleoprotein 40 kda protein (39 kDa, Protein from human)
- Rna helicase aquarius (171 kDa, Protein from human)
- Eukaryotic initiation factor 4a-iii (46 kDa, Protein from human)
- U5 snrna (37 kDa, RNA from human)
- U6 snrna (34 kDa, RNA from human)
- Small nuclear ribonucleoprotein f (9 kDa, Protein from human)
- U2 snrna (60 kDa, RNA from human)
- Zinc ion (65 Da, Ligand)
- Small nuclear ribonucleoprotein-associated proteins b and b' (24 kDa, Protein from human)
- Pleiotropic regulator 1 (57 kDa, Protein from human)
- 116 kda u5 small nuclear ribonucleoprotein component (109 kDa, Protein from human)
- Prkr-interacting protein 1 (21 kDa, Protein from human)
- Pre-mrna (14 kDa, RNA from unidentified adenovirus)
- Protein bud31 homolog (17 kDa, Protein from human)
- Protein fam32a (13 kDa, Protein from human)
- Small nuclear ribonucleoprotein g (8 kDa, Protein from human)
- Serine/arginine repetitive matrix protein 2 (300 kDa, Protein from human)
- Pre-mrna-splicing factor syf2 (28 kDa, Protein from human)
- Protein casc3 (76 kDa, Protein from human)
- Spliceosome-associated protein cwc15 homolog (26 kDa, Protein from human)
The cryo-EM structure of human C* complex
Resolution: 4.3 Å
EM Method: Single-particle
Fitted PDBs: 7w5b
Q-score: 0.192
Zhan X, Lu Y, Zhang X, Yan C, Shi Y
Mol Cell (2022) 82 pp. 2769-2778.e4 [ DOI: doi:10.1016/j.molcel.2022.05.021 Pubmed: 35705093 ]
- Pre-mrna-splicing factor cwc22 homolog (105 kDa, Protein from human)
- Pre-mrna (14 kDa, RNA from unidentified adenovirus)
- Crooked neck-like protein 1 (100 kDa, Protein from human)
- Cactin (88 kDa, Protein from human)
- Small nuclear ribonucleoprotein g (8 kDa, Protein from human)
- Small nuclear ribonucleoprotein sm d1 (13 kDa, Protein from human)
- Rna-binding protein 8a (19 kDa, Protein from human)
- Pre-mrna-processing factor 17 (65 kDa, Protein from human)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Pre-mrna-processing factor 19 (55 kDa, Protein from human)
- Protein mago nashi homolog (17 kDa, Protein from human)
- Small nuclear ribonucleoprotein f (9 kDa, Protein from human)
- Inositol hexakisphosphate (660 Da, Ligand)
- U5 small nuclear ribonucleoprotein 200 kda helicase (244 kDa, Protein from human)
- Small nuclear ribonucleoprotein sm d2 (13 kDa, Protein from human)
- Pleiotropic regulator 1 (57 kDa, Protein from human)
- Peptidyl-prolyl cis-trans isomerase-like 1 (18 kDa, Protein from human)
- U2 snrna (60 kDa, RNA from human)
- Pre-mrna-processing-splicing factor 8 (273 kDa, Protein from human)
- U6 snrna (34 kDa, RNA from human)
- Small nuclear ribonucleoprotein sm d3 (13 kDa, Protein from human)
- Zinc ion (65 Da, Ligand)
- Protein casc3 (76 kDa, Protein from human)
- Atp-dependent rna helicase dhx8 (139 kDa, Protein from human)
- Pre-mrna-splicing factor slu7 (68 kDa, Protein from human)
- Pre-mrna-splicing factor spf27 (26 kDa, Protein from human)
- Cell division cycle 5-like protein (92 kDa, Protein from human)
- Rna helicase aquarius (171 kDa, Protein from human)
- Prkr-interacting protein 1 (21 kDa, Protein from human)
- U2 small nuclear ribonucleoprotein b'' (25 kDa, Protein from human)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Spliceosome-associated protein cwc15 homolog (26 kDa, Protein from human)
- Protein fam32a (13 kDa, Protein from human)
- Pre-mrna-splicing factor rbm22 (46 kDa, Protein from human)
- Magnesium ion (24 Da, Ligand)
- U5 small nuclear ribonucleoprotein 40 kda protein (39 kDa, Protein from human)
- Small nuclear ribonucleoprotein e (10 kDa, Protein from human)
- Pre-mrna-splicing factor syf1 (100 kDa, Protein from human)
- 116 kda u5 small nuclear ribonucleoprotein component (109 kDa, Protein from human)
- Snw domain-containing protein 1 (61 kDa, Protein from human)
- Protein bud31 homolog (17 kDa, Protein from human)
- Nf-kappa-b-activating protein (47 kDa, Protein from human)
- Human pre-c*-i complex (Complex from Homo sapiens)
- Peptidyl-prolyl cis-trans isomerase e (33 kDa, Protein from human)
- U2 small nuclear ribonucleoprotein a' (28 kDa, Protein from human)
- Small nuclear ribonucleoprotein-associated proteins b and b' (24 kDa, Protein from human)
- Eukaryotic initiation factor 4a-iii (46 kDa, Protein from human)
- Pre-mrna-splicing factor syf2 (28 kDa, Protein from human)
- Serine/arginine repetitive matrix protein 2 (300 kDa, Protein from human)
- Pre-mrna (55 kDa, RNA from unidentified adenovirus)
- U5 snrna (37 kDa, RNA from human)
Cryo-EM Structure of the Activated Human Minor Spliceosome (minor Bact Complex)
Resolution: 2.89 Å
EM Method: Single-particle
Fitted PDBs: 7dvq
Q-score: 0.432
Bai R, Wan R, Wang L, Xu K, Zhang Q, Lei J, Shi Y
Science (2021) 371 [ DOI: doi:10.1126/science.abg0879 Pubmed: 33509932 ]
- Peptidyl-prolyl cis-trans isomerase cwc27 homolog (53 kDa, Protein from Human)
- Potassium ion (39 Da, Ligand)
- Splicing factor 3b subunit 3 (135 kDa, Protein from Human)
- 116 kda u5 small nuclear ribonucleoprotein component (109 kDa, Protein from Human)
- Pre-mrna-splicing factor cwc22 homolog (105 kDa, Protein from Human)
- U12 snrna (48 kDa, RNA from Homo sapiens)
- Rna-binding motif protein, x-linked 2 (37 kDa, Protein from Human)
- Small nuclear ribonucleoprotein sm d3 (13 kDa, Protein from Human)
- Small nuclear ribonucleoprotein sm d2 (13 kDa, Protein from Human)
- Serine/arginine repetitive matrix protein 1 (102 kDa, Protein from Human)
- Small nuclear ribonucleoprotein-associated proteins b and b' (24 kDa, Protein from Human)
- Splicing factor 3b subunit 6 (14 kDa, Protein from Human)
- Ring finger protein 113a (38 kDa, Protein from Human)
- Inositol hexakisphosphate (660 Da, Ligand)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Pre-mrna (44 kDa, RNA from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Smad nuclear-interacting protein 1 (45 kDa, Protein from Human)
- Splicing factor 3b subunit 4 (44 kDa, Protein from Human)
- G-patch domain and kow motifs-containing protein (52 kDa, Protein from Human)
- Splicing factor 3b subunit 5 (10 kDa, Protein from Human)
- 5'-o-[(s)-hydroxy{[(r)-hydroxy{[(s)-hydroxy(methoxy)phosphoryl]oxy}phosphoryl]oxy}phosphoryl]guanosine (537 Da, Ligand)
- The activated human minor spliceosome (human minor bact complex) (Complex from Homo sapiens)
- U5 small nuclear ribonucleoprotein 200 kda helicase (244 kDa, Protein from Human)
- Snw domain-containing protein 1 (61 kDa, Protein from Human)
- U5 snrna (37 kDa, RNA from Homo sapiens)
- Serine/arginine repetitive matrix protein 2 (300 kDa, Protein from Human)
- Pleiotropic regulator 1 (57 kDa, Protein from Human)
- Cell division cycle 5-like protein (92 kDa, Protein from Human)
- Splicing factor 3b subunit 2 (100 kDa, Protein from Human)
- Cysteine-rich pdz-binding protein (11 kDa, Protein from Human)
- Armadillo repeat-containing protein 7 (21 kDa, Protein from Human)
- Phd finger-like domain-containing protein 5a (12 kDa, Protein from Human)
- Zinc ion (65 Da, Ligand)
- Sodium channel modifier 1 (25 kDa, Protein from Human)
- Splicing factor 3b subunit 1 (146 kDa, Protein from Human)
- Rna-binding protein 48 (41 kDa, Protein from Human)
- U6atac snrna (39 kDa, RNA from Homo sapiens)
- Small nuclear ribonucleoprotein e (10 kDa, Protein from Human)
- Small nuclear ribonucleoprotein f (9 kDa, Protein from Human)
- Pre-mrna-processing-splicing factor 8 (273 kDa, Protein from Human)
- Water (18 Da, Ligand)
- Small nuclear ribonucleoprotein sm d1 (13 kDa, Protein from Human)
- Spliceosome-associated protein cwc15 homolog (26 kDa, Protein from Human)
- Ring-type e3 ubiquitin-protein ligase ppil2 (58 kDa, Protein from Human)
- Crooked neck-like protein 1 (100 kDa, Protein from Human)
- U5 small nuclear ribonucleoprotein 40 kda protein (39 kDa, Protein from Human)
- Small nuclear ribonucleoprotein g (8 kDa, Protein from Human)
- Pre-mrna-splicing factor atp-dependent rna helicase dhx16 (119 kDa, Protein from Human)
- Bud13 homolog (70 kDa, Protein from Human)
Resolution: 2.5 Å
EM Method: Single-particle
Fitted PDBs: 7dco
Q-score: 0.408
Bai R, Wan R, Yan C, Jia Q, Lei J, Shi Y
Science (2021) 371 [ DOI: doi:10.1126/science.abe8863 Pubmed: 33243853 ]
- U2 snrna (376 kDa, RNA from Saccharomyces cerevisiae)
- Pre-mrna-processing factor 19 (56 kDa, Protein from Saccharomyces cerevisiae)
- Brr2 isoform 1 (246 kDa, Protein from Saccharomyces cerevisiae)
- Pre-mrna-splicing factor slt11 (40 kDa, Protein from Saccharomyces cerevisiae)
- Cwc27 isoform 1 (35 kDa, Protein from Saccharomyces cerevisiae)
- Bj4_g0056610.mrna.1.cds.1 (12 kDa, Protein from Saccharomyces cerevisiae)
- Pre-mrna-splicing factor cwc21 (15 kDa, Protein from Saccharomyces cerevisiae)
- Hlj1_g0053790.mrna.1.cds.1 (27 kDa, Protein from Saccharomyces cerevisiae)
- Bj4_g0027490.mrna.1.cds.1 (12 kDa, Protein from Saccharomyces cerevisiae)
- Rse1 isoform 1 (153 kDa, Protein from Saccharomyces cerevisiae)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Hsh49 isoform 1 (24 kDa, Protein from Saccharomyces cerevisiae)
- Small nuclear ribonucleoprotein sm d3 (8 kDa, Protein from Saccharomyces cerevisiae)
- Prp9 isoform 1 (63 kDa, Protein from Saccharomyces cerevisiae)
- Inositol hexakisphosphate (660 Da, Ligand)
- Prp11 isoform 1 (29 kDa, Protein from Saccharomyces cerevisiae)
- Hlj1_g0054350.mrna.1.cds.1 (37 kDa, Protein from Saccharomyces cerevisiae)
- Sx2_g0027210.mrna.1.cds.1 (14 kDa, Protein from Saccharomyces cerevisiae)
- Cdc40 isoform 1 (52 kDa, Protein from Saccharomyces cerevisiae)
- Pre-mrna-splicing factor cwc24 (29 kDa, Protein from Saccharomyces cerevisiae)
- Snt309 isoform 1 (20 kDa, Protein from Saccharomyces cerevisiae)
- Pre-mrna-splicing factor atp-dependent rna helicase-like protein prp2 (99 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Prp8 isoform 1 (279 kDa, Protein from Saccharomyces cerevisiae)
- Small nuclear ribonucleoprotein g (8 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Bud31 isoform 1 (18 kDa, Protein from Saccharomyces cerevisiae)
- Bj4_g0037700.mrna.1.cds.1 (12 kDa, Protein from Saccharomyces cerevisiae)
- Small nuclear ribonucleoprotein e (10 kDa, Protein from Saccharomyces cerevisiae)
- Calcium ion (40 Da, Ligand)
- Pre-mrna-splicing factor cwc2 (29 kDa, Protein from Saccharomyces cerevisiae)
- Pre-mrna-splicing factor cwc26 (14 kDa, Protein from Saccharomyces cerevisiae)
- Cwc22 isoform 1 (67 kDa, Protein from Saccharomyces cerevisiae)
- Zinc ion (65 Da, Ligand)
- Hlj1_g0043010.mrna.1.cds.1 (27 kDa, Protein from Saccharomyces cerevisiae)
- Hsh155 isoform 1 (110 kDa, Protein from Saccharomyces cerevisiae)
- Syf1 isoform 1 (100 kDa, Protein from Saccharomyces cerevisiae)
- Small nuclear ribonucleoprotein sm d3 (11 kDa, Protein from Saccharomyces cerevisiae)
- Pre-mrna-splicing factor cwc15 (19 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Clf1 isoform 1 (82 kDa, Protein from Saccharomyces cerevisiae)
- Bj4_g0014900.mrna.1.cds.1 (22 kDa, Protein from Saccharomyces cerevisiae)
- Smd1 isoform 1 (16 kDa, Protein from Saccharomyces cerevisiae)
- Water (18 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- U5 snrna (68 kDa, RNA from Saccharomyces cerevisiae)
- Snu114 isoform 1 (114 kDa, Protein from Saccharomyces cerevisiae)
- Pre-mrna-processing protein 45 (42 kDa, Protein from Saccharomyces cerevisiae)
- Pre-mrna-splicing factor spp2 (20 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- The activated spliceosome bact complex (Complex from Saccharomyces cerevisiae)
- Pre-mrna-splicing factor cef1 (67 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Rds3 complex subunit 10 (9 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Pre-mrna leakage protein 1 (23 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Pre-mrna (44 kDa, RNA from Saccharomyces cerevisiae)
- Pre-mrna-splicing factor prp21 (33 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Sm protein f (9 kDa, Protein from Saccharomyces cerevisiae)
- U6 snrna (35 kDa, RNA from Saccharomyces cerevisiae)
Yeast C complex spliceosome at 2.8 Angstrom resolution with Prp18/Slu7 bound, composite map
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 7b9v
Q-score: 0.378
Wilkinson ME, Fica SM, Galej WP, Nagai K
Mol Cell (2021) 81 pp. 1439- [ Pubmed: 33705709 DOI: doi:10.1016/j.molcel.2021.02.021 ]
- Clf1 isoform 1 (82 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor cwc21 (15 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing helicase brr2 (246 kDa, Protein from Baker's yeast)
- 5' exon of ubc4 mrna (15 kDa, RNA from Saccharomyces cerevisiae)
- Pre-mrna-splicing factor snu114 (114 kDa, Protein from Baker's yeast)
- Syf1 isoform 1 (100 kDa, Protein from Baker's yeast)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Pre-mrna-splicing factor cwc25 (20 kDa, Protein from Baker's yeast)
- Bj4_g0054360.mrna.1.cds.1 (50 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor slt11 (40 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor syf2 (24 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor cwc15 (19 kDa, Protein from Baker's yeast)
- Hlj1_g0053790.mrna.1.cds.1 (27 kDa, Protein from Baker's yeast)
- Small nuclear ribonucleoprotein g (8 kDa, Protein from Baker's yeast)
- D-chiro inositol hexakisphosphate (660 Da, Ligand)
- Snt309 isoform 1 (20 kDa, Protein from Baker's yeast)
- Pre-mrna-processing factor 19 (56 kDa, Protein from Baker's yeast)
- Small nuclear ribonucleoprotein-associated protein b (22 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor isy1 (27 kDa, Protein from Baker's yeast)
- Potassium ion (39 Da, Ligand)
- Branched intron and 3' exon of ubc4 pre-mrna (30 kDa, RNA from Saccharomyces cerevisiae)
- Small nuclear ribonucleoprotein e (10 kDa, Protein from Baker's yeast)
- Zinc ion (65 Da, Ligand)
- Pre-mrna-splicing factor cwc2 (38 kDa, Protein from Baker's yeast)
- Cwc22 isoform 1 (67 kDa, Protein from Baker's yeast)
- Bj4_g0027490.mrna.1.cds.1 (12 kDa, Protein from Baker's yeast)
- Ntc20 isoform 1 (15 kDa, Protein from Baker's yeast)
- Pre-mrna-processing protein 45 (42 kDa, Protein from Baker's yeast)
- Splicing factor yju2 (33 kDa, Protein from Baker's yeast)
- Cdc40 isoform 1 (52 kDa, Protein from Baker's yeast)
- Small nuclear ribonucleoprotein f (9 kDa, Protein from Baker's yeast)
- Y55_g0042700.mrna.1.cds.1 (67 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor slu7 (44 kDa, Protein from Baker's yeast)
- Small nuclear ribonucleoprotein sm d1 (16 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor 18 (28 kDa, Protein from Baker's yeast)
- Magnesium ion (24 Da, Ligand)
- Pre-mrna-splicing factor atp-dependent rna helicase prp16 (121 kDa, Protein from Baker's yeast)
- U5 snrna (68 kDa, RNA from Saccharomyces cerevisiae)
- Unassigned structure (20 kDa, Protein from Saccharomyces cerevisiae)
- Small nuclear ribonucleoprotein sm d3 (11 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor 8 (279 kDa, Protein from Baker's yeast)
- Small nuclear ribonucleoprotein sm d2 (12 kDa, Protein from Baker's yeast)
- Bud31 isoform 1 (18 kDa, Protein from Baker's yeast)
- U2 snrna (376 kDa, RNA from Saccharomyces cerevisiae)
- Yeast c complex spliceosome with prp18/slu7 bound (2 MDa, Complex from Saccharomyces cerevisiae)
- U6 snrna (35 kDa, RNA from Saccharomyces cerevisiae)
Resolution: 8.0 Å
EM Method: Single-particle
Fitted PDBs: 7abi
Q-score: 0.145
Townsend C, Leelaram MN, Agafonov DE, Dybkov O, Will CL, Bertram K, Urlaub H, Kastner B, Stark H, Luhrmann R
Science (2020) 370 [ DOI: doi:10.1126/science.abc3753 Pubmed: 33243851 ]
- U6 snrna (34 kDa, RNA from Human)
- Splicing factor 3b subunit 1 (146 kDa, Protein from Human)
- 116 kda u5 small nuclear ribonucleoprotein component (109 kDa, Protein from Human)
- Splicing factor 3b subunit 6 (14 kDa, Protein from Human)
- Minx m3 pre-mrna (103 kDa, RNA from synthetic construct)
- Pre-mrna-splicing factor syf1 (100 kDa, Protein from Human)
- Pre-mrna-splicing factor 38a (37 kDa, Protein from Human)
- U2 small nuclear ribonucleoprotein b'' (25 kDa, Protein from Human)
- Rna-binding motif protein, x-linked 2 (37 kDa, Protein from Human)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Dna/rna-binding protein kin17 (45 kDa, Protein from Human)
- Small nuclear ribonucleoprotein sm d3 (13 kDa, Protein from Human)
- Splicing factor 3a subunit 2 (49 kDa, Protein from Human)
- Small nuclear ribonucleoprotein sm d2 (13 kDa, Protein from Human)
- Splicing factor 3a subunit 3 (58 kDa, Protein from Human)
- Serine/arginine repetitive matrix protein 1 (102 kDa, Protein from Human)
- U5 snrna (36 kDa, RNA from Human)
- Small nuclear ribonucleoprotein-associated proteins b and b' (24 kDa, Protein from Human)
- Zinc finger matrin-type protein 2 (23 kDa, Protein from Human)
- Magnesium ion (24 Da, Ligand)
- U5 small nuclear ribonucleoprotein 200 kda helicase (244 kDa, Protein from Human)
- Smad nuclear-interacting protein 1 (45 kDa, Protein from Human)
- Protein bud31 homolog (17 kDa, Protein from Human)
- Beta-catenin-like protein 1 (65 kDa, Protein from Human)
- Inositol hexakisphosphate (660 Da, Ligand)
- Phd finger-like domain-containing protein 5a (12 kDa, Protein from Human)
- Splicing factor 3b subunit 5 (10 kDa, Protein from Human)
- U5 small nuclear ribonucleoprotein 40 kda protein (39 kDa, Protein from Human)
- U2 small nuclear ribonucleoprotein a' (28 kDa, Protein from Human)
- Snw domain-containing protein 1 (61 kDa, Protein from Human)
- U2 snrna (60 kDa, RNA from Human)
- Human pre-bact-2 spliceosome (Complex from Homo sapiens)
- Pleiotropic regulator 1 (57 kDa, Protein from Human)
- Cell division cycle 5-like protein (92 kDa, Protein from Human)
- Splicing factor 3b subunit 2 (100 kDa, Protein from Human)
- Minx m3 pre-mrna (Complex from synthetic construct)
- Splicing factor 3b subunit 4 (44 kDa, Protein from Human)
- Microfibrillar-associated protein 1 (52 kDa, Protein from Human)
- Ubiquitin-like protein 5 (8 kDa, Protein from Human)
- Peptidyl-prolyl cis-trans isomerase e (33 kDa, Protein from Human)
- Small nuclear ribonucleoprotein f (9 kDa, Protein from Human)
- Pre-mrna-processing factor 17 (65 kDa, Protein from Human)
- Pre-mrna-processing-splicing factor 8 (273 kDa, Protein from Human)
- Small nuclear ribonucleoprotein e (10 kDa, Protein from Human)
- Small nuclear ribonucleoprotein sm d1 (13 kDa, Protein from Human)
- Splicing factor 3a subunit 1 (88 kDa, Protein from Human)
- Spliceosome-associated protein cwc15 homolog (26 kDa, Protein from Human)
- Intron-binding protein aquarius (171 kDa, Protein from Human)
- Ring-type e3 ubiquitin-protein ligase ppil2 (58 kDa, Protein from Human)
- Splicing factor 3b subunit 3 (135 kDa, Protein from Human)
- Crooked neck-like protein 1 (100 kDa, Protein from Human)
- Pre-mrna-splicing factor rbm22 (46 kDa, Protein from Human)
- Human pre-bact-2 spliceosome (Complex)
- Small nuclear ribonucleoprotein g (8 kDa, Protein from Human)
- Bud13 homolog (70 kDa, Protein from Human)