In situ outer dynein arm from Chlamydomonas reinhardtii in a pre-power stroke state
Resolution: 38.0 Å
EM Method: Subtomogram averaging
Fitted PDBs: 8bwy
Q-score: 0.039
Zimmermann N, Noga A, Obbineni JM, Ishikawa T
EMBO J (2023) 42 pp. e112466-e112466 [ DOI: doi:10.15252/embj.2022112466 Pubmed: 37051721 ]
- Docking complex 1/2 protein (11 kDa, Protein from Chlamydomonas reinhardtii)
- Dynein light chain (12 kDa, Protein from Chlamydomonas reinhardtii)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Outer arm dynein beta heavy chain (530 kDa, Protein from Chlamydomonas reinhardtii)
- Dynein-1-alpha heavy chain, flagellar inner arm i1 complex protein, putative (475 kDa, Protein from Chlamydomonas reinhardtii)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Calmodulin (35 kDa, Protein from Chlamydomonas reinhardtii)
- Dynein light chain roadblock-type 2 protein (14 kDa, Protein from Chlamydomonas reinhardtii)
- Dynein heavy chain, outer arm protein (534 kDa, Protein from Chlamydomonas reinhardtii)
- Dynein light chain (10 kDa, Protein from Chlamydomonas reinhardtii)
- Dynein light chain (12 kDa, Protein from Chlamydomonas reinhardtii)
- Dynein light chain roadblock-type 2 protein (18 kDa, Protein from Chlamydomonas reinhardtii)
- Dynein light chain (13 kDa, Protein from Chlamydomonas reinhardtii)
- Dynein light chain tctex-type 1 protein (13 kDa, Protein from Chlamydomonas reinhardtii)
- Thioredoxin (12 kDa, Protein from Chlamydomonas reinhardtii)
- In situ outer dynein arm (Cellular component from Chlamydomonas reinhardtii)
- Flagellar outer dynein arm intermediate protein, putative (77 kDa, Protein from Chlamydomonas reinhardtii)
- Dynein light chain (10 kDa, Protein from Chlamydomonas reinhardtii)
- Dynein light chain 2a (15 kDa, Protein from Chlamydomonas reinhardtii)
- Dynein intermediate chain 2 (77 kDa, Protein from Chlamydomonas reinhardtii)
- Dynein light chain (10 kDa, Protein from Chlamydomonas reinhardtii)
In situ outer dynein arm from Chlamydomonas reinhardtii in the post-power stroke state
Resolution: 30.3 Å
EM Method: Subtomogram averaging
Fitted PDBs: 8bx8
Q-score: 0.043
Zimmermann N, Noga A, Obbineni JM, Ishikawa T
EMBO J (2023) 42 pp. e112466-e112466 [ DOI: doi:10.15252/embj.2022112466 Pubmed: 37051721 ]
- Dynein light chain (10 kDa, Protein from Tetrahymena thermophila)
- Dynein light chain roadblock-type 2 protein (14 kDa, Protein from Tetrahymena thermophila)
- Outer arm dynein beta heavy chain (530 kDa, Protein from Tetrahymena thermophila)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Dynein light chain 2a (15 kDa, Protein from Tetrahymena thermophila)
- Dynein intermediate chain 2 (76 kDa, Protein from Tetrahymena thermophila)
- In situ outer dynein arm (Cellular component from Chlamydomonas reinhardtii)
- Dynein-1-alpha heavy chain, flagellar inner arm i1 complex protein, putative (475 kDa, Protein from Tetrahymena thermophila)
- Dynein light chain tctex-type 1 protein (13 kDa, Protein from Tetrahymena thermophila)
- Dynein light chain (12 kDa, Protein from Tetrahymena thermophila)
- Dynein light chain (12 kDa, Protein from Tetrahymena thermophila)
- Dynein light chain (11 kDa, Protein from Tetrahymena thermophila)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Dynein heavy chain, outer arm protein (534 kDa, Protein from Tetrahymena thermophila)
- Dynein light chain 4a (17 kDa, Protein from Tetrahymena thermophila)
- Magnesium ion (24 Da, Ligand)
- Dynein light chain (10 kDa, Protein from Tetrahymena thermophila)
- Flagellar outer dynein arm intermediate protein, putative (77 kDa, Protein from Tetrahymena thermophila)
- Dynein light chain 1 (22 kDa, Protein from Tetrahymena thermophila)
- Dynein light chain roadblock-type 2 protein (18 kDa, Protein from Tetrahymena thermophila)
- Dynein light chain (10 kDa, Protein from Tetrahymena thermophila)
- Thioredoxin (14 kDa, Protein from Tetrahymena thermophila)
Structure of outer-arm dyneins bound to microtubule with microtubule binding state 1(MTBS-1)
Resolution: 4.0 Å
EM Method: Single-particle
Fitted PDBs: 7k58
Q-score: 0.357
Rao Q, Han L, Wang Y, Chai P, Kuo YW, Yang R, Hu F, Yang Y, Howard J, Zhang K
Nat Struct Mol Biol (2021) 28 pp. 799-810 [ DOI: doi:10.1038/s41594-021-00656-9 Pubmed: 34556869 ]
- Thioredoxin (12 kDa, Protein from Tetrahymena thermophila)
- Dynein intermediate chain 2 (69 kDa, Protein from Tetrahymena thermophila)
- Dynein light chain (11 kDa, Protein from Tetrahymena thermophila)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Dynein light chain (10 kDa, Protein from Tetrahymena thermophila)
- Flagellar outer dynein arm intermediate protein, putative (63 kDa, Protein from Tetrahymena thermophila)
- Dynein light chain 2a (14 kDa, Protein from Tetrahymena thermophila)
- Dynein light chain tctex-type 1 protein (12 kDa, Protein from Tetrahymena thermophila)
- Dynein light chain (10 kDa, Protein from Tetrahymena thermophila)
- Dynein light chain (11 kDa, Protein from Tetrahymena thermophila)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Outer arm dynein beta heavy chain (529 kDa, Protein from Tetrahymena thermophila)
- Dynein light chain roadblock-type 2 protein (12 kDa, Protein from Tetrahymena thermophila)
- Magnesium ion (24 Da, Ligand)
- Dynein light chain (10 kDa, Protein from Tetrahymena thermophila)
- Outer-arm dynein (2 MDa, Complex from Tetrahymena thermophila)
- Dynein light chain roadblock (10 kDa, Protein from Tetrahymena thermophila)
- Dynein light chain (11 kDa, Protein from Tetrahymena thermophila)
- Dynein heavy chain, outer arm protein (533 kDa, Protein from Tetrahymena thermophila)
- Dynein light chain 1 (21 kDa, Protein from Tetrahymena thermophila)
- Gamma heavy chain (450 kDa, Protein from Tetrahymena thermophila)
Structure of outer-arm dynein bound to microtubule doublet in microtubule binding state 2 (MTBS-2)
Resolution: 4.5 Å
EM Method: Single-particle
Fitted PDBs: 7k5b
Q-score: 0.264
Rao Q, Han L, Wang Y, Chai P, Kuo YW, Yang R, Hu F, Yang Y, Howard J, Zhang K
Nat Struct Mol Biol (2021) 28 pp. 799-810 [ DOI: doi:10.1038/s41594-021-00656-9 Pubmed: 34556869 ]
- Dynein heavy chain, outer arm protein (533 kDa, Protein from Tetrahymena thermophila)
- Thioredoxin (12 kDa, Protein from Tetrahymena thermophila)
- Dynein intermediate chain 2 (69 kDa, Protein from Tetrahymena thermophila)
- Dynein light chain (11 kDa, Protein from Tetrahymena thermophila)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Outer-arm dynein bound to microtubule doublet in microtubule binding state 2 (mtbs-2) (Complex from Tetrahymena thermophila)
- Dynein light chain (10 kDa, Protein from Tetrahymena thermophila)
- Flagellar outer dynein arm intermediate protein, putative (63 kDa, Protein from Tetrahymena thermophila)
- Dynein light chain 2a (14 kDa, Protein from Tetrahymena thermophila)
- Dynein light chain roadblock-type 2 protein (14 kDa, Protein from Tetrahymena thermophila)
- Dynein light chain (12 kDa, Protein from Tetrahymena thermophila)
- Dynein light chain (11 kDa, Protein from Tetrahymena thermophila)
- Dynein light chain (10 kDa, Protein from Tetrahymena thermophila)
- Outer arm dynein beta heavy chain (529 kDa, Protein from Tetrahymena thermophila)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Dynein light chain roadblock-type 2 protein (17 kDa, Protein from Tetrahymena thermophila)
- Dynein light chain 1 (21 kDa, Protein from Tetrahymena thermophila)
- Dynein light chain 4a (16 kDa, Protein from Tetrahymena thermophila)
- Magnesium ion (24 Da, Ligand)
- Dynein light chain (10 kDa, Protein from Tetrahymena thermophila)
- Gamma heavy chain (450 kDa, Protein from Tetrahymena thermophila)
- Dynein light chain tctex-type 1 protein (12 kDa, Protein from Tetrahymena thermophila)
Structure of the free outer-arm dynein in pre-parallel state
Resolution: 8.0 Å
EM Method: Single-particle
Fitted PDBs: 7kek
Q-score: 0.082
Rao Q, Han L, Wang Y, Chai P, Kuo YW, Yang R, Hu F, Yang Y, Howard J, Zhang K
Nat Struct Mol Biol (2021) 28 pp. 799-810 [ DOI: doi:10.1038/s41594-021-00656-9 Pubmed: 34556869 ]
- Dynein light chain tctex_b (13 kDa, Protein from Tetrahymena thermophila)
- Dynein gamma heavy chain (475 kDa, Protein from Tetrahymena thermophila)
- Dynein alpha heavy chain (534 kDa, Protein from Tetrahymena thermophila)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Dynein intermediate chain dic2 (77 kDa, Protein from Tetrahymena thermophila)
- Dynein light chain 1 (22 kDa, Protein from Tetrahymena thermophila)
- Dynein light chain roadblock lc7b (18 kDa, Protein from Tetrahymena thermophila)
- Dynein light chain lc8_2b (13 kDa, Protein from Tetrahymena thermophila)
- Dynein light chain lc8_2a (lc8e) (10 kDa, Protein from Tetrahymena thermophila)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Dynein beta heavy chain (530 kDa, Protein from Tetrahymena thermophila)
- Dynein light chain lc8_3b (12 kDa, Protein from Tetrahymena thermophila)
- Dynein light chain tctex_a (lc2a) (15 kDa, Protein from Tetrahymena thermophila)
- Dynein light chain roadblock lc7a (14 kDa, Protein from Tetrahymena thermophila)
- Dynein light chain lc8_3a (10 kDa, Protein from Tetrahymena thermophila)
- Free outer-arm dynein (Complex from Tetrahymena thermophila)
- Dynein intermediate chain dic3 (77 kDa, Protein from Tetrahymena thermophila)
- Dynein light chain lc8_1a (lc10) (12 kDa, Protein from Tetrahymena thermophila)
- Magnesium ion (24 Da, Ligand)
- Dynein light chain lc8_1b (dlc82) (10 kDa, Protein from Tetrahymena thermophila)
- Thioredoxin lc3bl (14 kDa, Protein from Tetrahymena thermophila)
The structure of the Tetrahymena thermophila outer dynein arm on doublet microtubule
Resolution: 8.0 Å
EM Method: Single-particle
Fitted PDBs: 7moq
Q-score: 0.067
Kubo S, Yang SK, Black CS, Dai D, Valente-Paterno M, Gaertig J, Ichikawa M, Bui KH
EMBO Rep (2021) 22 pp. e52911-e52911 [ DOI: doi:10.15252/embr.202152911 Pubmed: 34338432 ]
- Dynein light chain (10 kDa, Protein from Tetrahymena thermophila CU428)
- Outer dynein arm docking complex protein oda protein (63 kDa, Protein from Tetrahymena thermophila CU428)
- Dynein light chain tctex-type 1 protein (13 kDa, Protein from Tetrahymena thermophila CU428)
- Docking complex 1/2 protein (11 kDa, Protein from Tetrahymena thermophila CU428)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Tubulin alpha chain (49 kDa, Protein from Tetrahymena thermophila CU428)
- Dynein light chain 2a (15 kDa, Protein from Tetrahymena thermophila CU428)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Thioredoxin (12 kDa, Protein from Tetrahymena thermophila CU428)
- Dynein-1-alpha heavy chain, flagellar inner arm i1 complex protein, putative (475 kDa, Protein from Tetrahymena thermophila CU428)
- Dynein light chain (12 kDa, Protein from Tetrahymena thermophila CU428)
- Outer dynein arm complex from tetrahymena thermophila (2 MDa, Cellular component from Tetrahymena thermophila CU428)
- Outer arm dynein beta heavy chain (530 kDa, Protein from Tetrahymena thermophila CU428)
- Dynein light chain (12 kDa, Protein from Tetrahymena thermophila CU428)
- Dynein intermediate chain 2 (77 kDa, Protein from Tetrahymena thermophila CU428)
- Dynein light chain (10 kDa, Protein from Tetrahymena thermophila CU428)
- Outer dynein arm docking complex protein oda protein (35 kDa, Protein from Tetrahymena thermophila CU428)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Dynein light chain roadblock-type 2 protein (18 kDa, Protein from Tetrahymena thermophila CU428)
- Dynein light chain (13 kDa, Protein from Tetrahymena thermophila CU428)
- Magnesium ion (24 Da, Ligand)
- Dynein light chain roadblock-type 2 protein (14 kDa, Protein from Tetrahymena thermophila CU428)
- Flagellar outer dynein arm intermediate protein, putative (77 kDa, Protein from Tetrahymena thermophila CU428)
- Tubulin beta chain (49 kDa, Protein from Tetrahymena thermophila CU428)
- Docking complex 1 protein (64 kDa, Protein from Tetrahymena thermophila CU428)
- Dynein heavy chain, outer arm protein (534 kDa, Protein from Tetrahymena thermophila CU428)
- Dynein light chain (10 kDa, Protein from Tetrahymena thermophila CU428)
Outer Dynein Arm-Shulin complex - overall structure (Tetrahymena thermophila)
Resolution: 8.78 Å
EM Method: Single-particle
Fitted PDBs: 6zyw
Q-score: 0.103
Mali GR, Ali FA, Lau CK, Begum F, Boulanger J, Howe JD, Chen ZA, Rappsilber J, Skehel M, Carter AP
Science (2021) 371 pp. 910-916 [ Pubmed: 33632841 DOI: doi:10.1126/science.abe0526 ]
- Dynein-1-alpha heavy chain, flagellar inner arm i1 complex protein, putative (475 kDa, Protein from Tetrahymena thermophila SB210)
- Shulin (139 kDa, Protein from Tetrahymena thermophila SB210)
- Dynein heavy chain, outer arm protein (534 kDa, Protein from Tetrahymena thermophila SB210)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Dynein light chain (10 kDa, Protein from Tetrahymena thermophila SB210)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Tetrahymena thermophila oda dyh4 motor (1 MDa, Complex)
- Tetrahymena thermophila oda dyh4 motor (Complex from Tetrahymena thermophila (strain SB210))
- Dynein light chain roadblock-type 2 protein (14 kDa, Protein from Tetrahymena thermophila SB210)
- Outer arm dynein beta heavy chain (530 kDa, Protein from Tetrahymena thermophila SB210)
- Flagellar outer dynein arm intermediate protein, putative (77 kDa, Protein from Tetrahymena thermophila SB210)
- Dynein light chain (12 kDa, Protein from Tetrahymena thermophila SB210)
- Dynein light chain roadblock-type 2 protein (11 kDa, Protein from Tetrahymena thermophila SB210)
- Dynein light chain (10 kDa, Protein from Tetrahymena thermophila SB210)
- Shulin (Complex from Tetrahymena thermophila (strain SB210))
- Dynein light chain (13 kDa, Protein from Tetrahymena thermophila SB210)
- Dynein intermediate chain 2 (77 kDa, Protein from Tetrahymena thermophila SB210)
- Dynein light chain (10 kDa, Protein from Tetrahymena thermophila SB210)
- Dynein light chain 2a (15 kDa, Protein from Tetrahymena thermophila SB210)
- Thioredoxin (12 kDa, Protein from Tetrahymena thermophila SB210)
- Dynein light chain (12 kDa, Protein from Tetrahymena thermophila SB210)
- Dynein light chain tctex-type 1 protein (13 kDa, Protein from Tetrahymena thermophila SB210)
Outer Dynein Arm-Shulin complex - Shulin region from Tetrahymena thermophila
Resolution: 4.3 Å
EM Method: Single-particle
Fitted PDBs: 6zyx
Q-score: 0.398
Mali GR, Ali FA, Lau CK, Begum F, Boulanger J, Howe JD, Chen ZA, Rappsilber J, Skehel M, Carter AP
Science (2021) 371 pp. 910-916 [ DOI: doi:10.1126/science.abe0526 Pubmed: 33632841 ]
- Dynein light chain (10 kDa, Protein from Tetrahymena thermophila CU428)
- Dynein light chain (12 kDa, Protein from Tetrahymena thermophila CU428)
- Dynein light chain (10 kDa, Protein from Tetrahymena thermophila CU428)
- Shulin (139 kDa, Protein from Tetrahymena thermophila CU428)
- Dynein intermediate chain 2 (77 kDa, Protein from Tetrahymena thermophila CU428)
- Flagellar outer dynein arm intermediate protein, putative (77 kDa, Protein from Tetrahymena thermophila CU428)
- Shulin (Complex from Tetrahymena thermophila CU428)
- Dynein light chain tctex-type 1 protein (13 kDa, Protein from Tetrahymena thermophila CU428)
- Dynein light chain 2a (15 kDa, Protein from Tetrahymena thermophila CU428)
- Dynein (Complex from Tetrahymena thermophila CU428)
- Dynein heavy chain, outer arm protein (534 kDa, Protein from Tetrahymena thermophila CU428)
- Dynein light chain (13 kDa, Protein from Tetrahymena thermophila CU428)
- Shulin region from outer dynein arm-shulin complex (tetrahymena thermophila). contains shulin (excluding c3 finger), lc8e, lc8f, lc8d, lc8, lc2a, lc9, dic n-termini and portion of dyh3 tail. (Complex)
- Guanosine-5'-triphosphate (523 Da, Ligand)
Full length human cytoplasmic dynein-1 in the phi-particle conformation
Resolution: 15.0 Å
EM Method: Single-particle
Fitted PDBs: 5nvu
Q-score: 0.087
Zhang K, Foster HE, Rondelet A, Lacey SE, Bahi-Buisson N, Bird AW, Carter AP
Cell (2017) 169 pp. 1303-1314.e18 [ DOI: doi:10.1016/j.cell.2017.05.025 Pubmed: 28602352 ]
- Dynein tail heavy chain (76 kDa, Protein from Homo sapiens)
- Lc8 (7 kDa, Protein from Homo sapiens)
- Robl (10 kDa, Protein from Homo sapiens)
- N-terminal dimerization domain (10 kDa, Protein from Homo sapiens)
- Tctex (8 kDa, Protein from Homo sapiens)
- Complete human cytoplasmic dynein-1 in the phi-particle conformation (Complex from Homo sapiens)
- N-terminal dimerization domain (10 kDa, Protein from Homo sapiens)
- Intermediate chain n-terminus peptides (2 kDa, Protein from Homo sapiens)
- Dynein intermediate chain (29 kDa, Protein from Homo sapiens)
- Dynein light intermediate chain (25 kDa, Protein from Homo sapiens)
- Dynein light intermediate chain (25 kDa, Protein from Homo sapiens)
- Dynein motor domain (269 kDa, Protein from Homo sapiens)
- Tctex (8 kDa, Protein from Homo sapiens)
- Dynein tail heavy chain (79 kDa, Protein from Homo sapiens)
- Intermediate chain n-terminus peptides (2 kDa, Protein from Homo sapiens)
Human cytoplasmic dynein-1 bound to dynactin and an N-terminal construct of BICD2
Resolution: 8.7 Å
EM Method: Single-particle
Fitted PDBs: 5nw4
Q-score: 0.113
Zhang K, Foster HE, Rondelet A, Lacey SE, Bahi-Buisson N, Bird AW, Carter AP
Cell (2017) 169 pp. 1303-1314.e18 [ Pubmed: 28602352 DOI: doi:10.1016/j.cell.2017.05.025 ]
- Dynein heavy chain (75 kDa, Protein from Homo sapiens)
- Dynactin 6 (20 kDa, Protein from Pig)
- Dynein intermediate chain (29 kDa, Protein from Homo sapiens)
- Dynactin (Complex from Sus scrofa)
- Dynactin (7 kDa, Protein from Pig)
- Arp11 (46 kDa, Protein from Pig)
- Dynactin (2 kDa, Protein from Pig)
- Capping protein (actin filament) muscle z-line, alpha 1 (33 kDa, Protein from Pig)
- Robl (10 kDa, Protein from Homo sapiens)
- Dynein heavy chain (78 kDa, Protein from Homo sapiens)
- Dynactin shoulder complex (14 kDa, Protein from Pig)
- Arp1 (42 kDa, Protein from Pig)
- Dynein intermediate chain (29 kDa, Protein from Homo sapiens)
- P150 (4 kDa, Protein from Pig)
- Bicd2n (23 kDa, Protein from Homo sapiens)
- Dynein-1 (Complex from Homo sapiens)
- Dynactin (3 kDa, Protein from Pig)
- Dynactin shoulder complex (48 kDa, Protein from Pig)
- Dynactin subunit 5 (24 kDa, Protein from Pig)
- Dynein n-terminal dimerization domain (10 kDa, Protein from Homo sapiens)
- Dynactin shoulder complex (51 kDa, Protein from Pig)
- Dynein n-terminal dimerization domain (10 kDa, Protein from Homo sapiens)
- Dynactin pointed end p62 (20 kDa, Protein from Pig)
- Human cytoplasmic dynein-1 bound to dynactin and an n-terminal construct of bicd2 (2 MDa, Complex)
- Dynactin shoulder complex (11 kDa, Protein from Pig)
- Bicd2 (Complex from Homo sapiens)
- Beta-actin (43 kDa, Protein from Pig)
- Dynein light intermediate chain (25 kDa, Protein from Homo sapiens)
- Dynein light intermediate chain (25 kDa, Protein from Homo sapiens)
- F-actin capping protein beta subunit variant ii (33 kDa, Protein from Pig)
- Dynactin p150 (4 kDa, Protein from Pig)
- Dynactin (5 kDa, Protein from Pig)