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sample_name:"Mediator of RNA polymerase II transcription subunit 7" AND software_ft:"RELION"
STRUCTURE OF YEAST TRANSCRIPTION PRE-INITIATION COMPLEX WITH TFIIH AND CORE MEDIATOR
Release date: May 9, 2018
Resolution: 5.8 Å
EM Method: Single-particle
Fitted PDBs: 5oqm
Q-score: 0.183
Resolution: 5.8 Å
EM Method: Single-particle
Fitted PDBs: 5oqm
Q-score: 0.183
Schilbach S, Hantsche M, Tegunov D, Dienemann C, Wigge C, Urlaub H, Cramer P
Nature (2017) 551 pp. 204-209 [ DOI: doi:10.1038/nature24282 Pubmed: 29088706 ]
Sample:
- Dna-directed rna polymerase ii subunit rpb4 (25 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Mediator of rna polymerase ii transcription subunit 11 (13 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Mediator of rna polymerase ii transcription subunit 14 (84 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Template dna (32 kDa, DNA from synthetic construct)
- Pre-initiation complex with core mediator (pic-cmed) (1 MDa, Complex)
- Unknown protein (3 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- General transcription and dna repair factor iih subunit tfb4 (35 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Mediator of rna polymerase ii transcription subunit 7 (25 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Mediator of rna polymerase ii transcription subunit 17 (78 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- General transcription and dna repair factor iih helicase subunit xpd (89 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- General transcription and dna repair factor iih subunit ssl1 (51 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Dna-directed rna polymerase ii subunit rpb11 (13 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Mediator of rna polymerase ii transcription subunit 9 (17 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Dna-directed rna polymerase ii subunit rpb9 (14 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Mediator of rna polymerase ii transcription (Complex from Saccharomyces cerevisiae)
- Transcription initiation factor iif subunit alpha (83 kDa, Protein from Saccharomyces cerevisiae)
- Transcription initiation factor iie subunit beta (37 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Dna-directed rna polymerase ii subunit rpb2 (138 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Mediator of rna polymerase ii transcription subunit 8 (25 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Dna-directed rna polymerase ii subunit rpb1 (191 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Dna-directed rna polymerases i, ii, and iii subunit rpabc4 (7 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Transcription initiation factor iia large subunit (32 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Transcription initiation factor iia subunit 2 (13 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Mediator of rna polymerase ii transcription subunit 31,mediator of rna polymerase ii transcription subunit 31 (16 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- General transcription and dna repair factor iih helicase subunit xpb (94 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Mediator of rna polymerase ii transcription subunit 21 (16 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase (Complex from Saccharomyces cerevisiae)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc3 (16 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Mediator of rna polymerase ii transcription subunit 6 (32 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Mediator of rna polymerase ii transcription subunit 10 (17 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Mediator of rna polymerase ii transcription subunit 19 (24 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Transcription initiation factor iib (38 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Mediator of rna polymerase ii transcription subunit 22 (13 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Nontemplate dna (32 kDa, DNA from synthetic construct)
- Dna (Complex from synthetic construct)
- Rna polymerase ii transcription factor b (Complex from Saccharomyces cerevisiae)
- Dna-directed rna polymerase ii subunit rpb3 (35 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- General transcription and dna repair factor iih subunit tfb1 (53 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Dna-directed rna polymerases i, ii, and iii subunit rpabc2 (17 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Transcription factor tfiie subunit (59 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Magnesium ion (24 Da, Ligand)
- Mediator of rna polymerase ii transcription subunit 18 (34 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Iron/sulfur cluster (351 Da, Ligand)
- General transcription and dna repair factor iih subunit tfb2 (60 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Tata-box-binding protein (27 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Rna polymerase ii transcription factor b subunit 3 (38 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Dna-directed rna polymerases i, ii, and iii subunit rpabc1 (25 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Mediator of rna polymerase ii transcription subunit 4 (33 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- General transcription and dna repair factor iih subunit tfb5 (8 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Dna-directed rna polymerases i, ii, and iii subunit rpabc5 (8 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Dna-directed rna polymerase ii subunit rpb7 (19 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Mediator of rna polymerase ii transcription subunit 20 (22 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Transcription initiation factor iif subunit beta (46 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Mediator-RNA Polymerase II Pre-Initiation Complex Delta Mediator Tail (Focused Refinement)
Release date: Sept. 14, 2016
Resolution: 15.3 Å
EM Method: Single-particle
Fitted PDBs: 5sva
Q-score: 0.049
Resolution: 15.3 Å
EM Method: Single-particle
Fitted PDBs: 5sva
Q-score: 0.049
Robinson PJ, Trnka MJ, Bushnell DA, Davis RE, Mattei PJ, Burlingame AL, Kornberg RD
Cell (2016) 166 pp. 1411-1422.e16 [ Pubmed: 27610567 DOI: doi:10.1016/j.cell.2016.08.050 ]
Sample:
- Dna-directed rna polymerase ii subunit rpb2 (Protein from yeast)
- Mediator of rna polymerase ii transcription subunit 1 (Protein from yeast)
- Dna-directed rna polymerase ii subunit rpb3 (Protein from yeast)
- Mediator of rna polymerase ii transcription subunit 4 (Protein from yeast)
- Transcription initiation factor iif subunit alpha (Protein from yeast)
- Mediator of rna polymerase ii transcription subunit 20 (Protein from yeast)
- Mediator-rna polymerase ii pre-initiation complex delta mediator tail (1 MDa, Complex)
- Rna polymerase ii transcription factor b subunit 2 (Protein from yeast)
- Mediator of rna polymerase ii transcription subunit 10 (Protein from yeast)
- 108 bp his4 promoter fragment (DNA from Saccharomyces cerevisiae)
- Mediator of rna polymerase ii transcription subunit 21 (Protein from yeast)
- Transcription initiation factor iia large subunit (Protein from Saccharomyces cerevisiae)
- Rna polymerase ii transcription factor b subunit 3 (Protein from yeast)
- Dna-directed rna polymerase ii subunit rpb11 (Protein from yeast)
- Dna repair helicase rad3 (Protein from yeast)
- Transcription initiation factor iib (Protein from Saccharomyces cerevisiae)
- Mediator of rna polymerase ii transcription subunit 9 (Protein from yeast)
- Transcription factor tfiie subunit tfa1 (Protein from Saccharomyces cerevisiae)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc 2 (Protein from yeast)
- Mediator of rna polymerase ii transcription subunit 31 (Protein from yeast)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc 4 (Protein from yeast)
- Dna-directed rna polymerase ii subunit rpb7 (Protein from yeast)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc3 (Protein from yeast)
- Transcription initiation factor iia subunit 2 (Protein from Saccharomyces cerevisiae)
- Dna repair helicase rad25 (Protein from yeast)
- Mediator of rna polymerase ii transcription subunit 6 (Protein from yeast)
- Transcription initiation factor iif subunit gamma (Protein from yeast)
- Mediator of rna polymerase ii transcription subunit 17 (Protein from yeast)
- Rna polymerase ii transcription factor b subunit 1 (Protein from yeast)
- Mediator of rna polymerase ii transcription subunit 8 (Protein from yeast)
- Tata-box-binding protein (Protein from Saccharomyces cerevisiae)
- Mediator of rna polymerase ii transcription subunit 11 (Protein from yeast)
- Serine/threonine-protein kinase kin28 (Protein from yeast)
- Dna-directed rna polymerase ii subunit rpb9 (Protein from yeast)
- Suppressor of stem-loop protein 1 (Protein from yeast)
- Rna polymerase ii transcription factor b subunit 5 (Protein from yeast)
- Transcription initiation factor iif subunit beta (Protein from yeast)
- Dna-directed rna polymerase ii subunit rpb1 (Protein from yeast)
- Transcription factor tfiie subunit tfa2 (Protein from Saccharomyces cerevisiae)
- Mediator of rna polymerase ii transcription subunit 18 (Protein from yeast)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc 5 (Protein from yeast)
- Mediator of rna polymerase ii transcription subunit 19 (Protein from yeast)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc 1 (Protein from yeast)
- Mediator of rna polymerase ii transcription subunit 7 (Protein from yeast)
- Mediator of rna polymerase ii transcription subunit 22 (Protein from yeast)
- Mediator of rna polymerase ii transcription subunit 14 (Protein from yeast)
- Rna polymerase ii transcription factor b subunit 4 (Protein from yeast)
- Dna-directed rna polymerase ii subunit rpb4 (Protein from yeast)
- Cyclin ccl1 (Protein from yeast)
Mediator-RNA Polymerase II Pre-Initiation Complex Delta TFIIE-IIH (Focused refinement)
Robinson PJ, Trnka MJ, Bushnell DA, Davis RE, Mattei PJ, Burlingame AL, Kornberg RD
Cell (2016) 166 pp. 1411-1422.e16 [ DOI: doi:10.1016/j.cell.2016.08.050 Pubmed: 27610567 ]
Sample:
- Mediator of rna polymerase ii transcription subunit 4 (Protein from yeast)
- Mediator-rna polymerase ii pre-initiation complex delta tfiie-iih (focused refinement) (1 MDa, Complex)
- Mediator of rna polymerase ii transcription subunit 18 (Protein from yeast)
- Dna-directed rna polymerase ii subunit rpb7 (Protein from yeast)
- Dna-directed rna polymerase ii subunit rpb9 (Protein from yeast)
- Mediator of rna polymerase ii transcription subunit 8 (Protein from yeast)
- Dna-directed rna polymerase ii subunit rpb2 (Protein from yeast)
- Dna-directed rna polymerase ii subunit rpb3 (Protein from yeast)
- Tata-box-binding protein (Protein from Saccharomyces cerevisiae)
- Mediator of rna polymerase ii transcription subunit 14 (Protein from yeast)
- Mediator of rna polymerase ii transcription subunit 21 (Protein from yeast)
- Mediator of rna polymerase ii transcription subunit 16 (Protein from yeast)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc 4 (Protein from yeast)
- Transcription initiation factor iia subunit 2 (Protein from Saccharomyces cerevisiae)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc 1 (Protein from yeast)
- Mediator of rna polymerase ii transcription subunit 6 (Protein from yeast)
- Mediator of rna polymerase ii transcription subunit 11 (Protein from yeast)
- Mediator of rna polymerase ii transcription subunit 31 (Protein from yeast)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc 2 (Protein from yeast)
- Transcription initiation factor iib (Protein from Saccharomyces cerevisiae)
- Mediator of rna polymerase ii transcription subunit 3 (Protein from yeast)
- Mediator of rna polymerase ii transcription subunit 5 (Protein from yeast)
- Mediator of rna polymerase ii transcription subunit 2 (Protein from yeast)
- Mediator of rna polymerase ii transcription subunit 15 (Protein from yeast)
- Mediator of rna polymerase ii transcription subunit 9 (Protein from yeast)
- 108 bp his4 promoter fragment (DNA from Saccharomyces cerevisiae)
- Mediator of rna polymerase ii transcription subunit 10 (Protein from yeast)
- Mediator of rna polymerase ii transcription subunit 22 (Protein from yeast)
- Transcription initiation factor iif subunit beta (Protein from yeast)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc5 (Protein from yeast)
- Mediator of rna polymerase ii transcription subunit 20 (Protein from yeast)
- Dna-directed rna polymerase ii subunit rpb4 (Protein from yeast)
- Mediator of rna polymerase ii transcription subunit 19 (Protein from yeast)
- Dna-directed rna polymerase ii subunit rpb1 (Protein from yeast)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc3 (Protein from yeast)
- Transcription initiation factor iia large subunit (Protein from Saccharomyces cerevisiae)
- Transcription initiation factor iif subunit alpha (Protein from yeast)
- Mediator of rna polymerase ii transcription subunit 7 (Protein from yeast)
- Mediator of rna polymerase ii transcription subunit 17 (Protein from yeast)
- Mediator of rna polymerase ii transcription subunit 1 (Protein from yeast)
- Transcription initiation factor iif subunit gamma (Protein from yeast)
- Dna-directed rna polymerase ii subunit rpb11 (Protein from yeast)
Mediator-RNA Polymerase II Pre-Initiation Complex
Robinson PJ, Trnka MJ, Bushnell DA, Davis RE, Mattei PJ, Burlingame AL, Kornberg RD
Cell (2016) 166 pp. 1411-1422.e16 [ Pubmed: 27610567 DOI: doi:10.1016/j.cell.2016.08.050 ]
Sample:
- Mediator of rna polymerase ii transcription subunit 1 (Protein from yeast)
- Mediator of rna polymerase ii transcription subunit 4 (Protein from yeast)
- Transcription initiation factor iif subunit alpha (Protein from yeast)
- Mediator-rna polymerase ii pre-initiation complex (2 MDa, Complex)
- Mediator of rna polymerase ii transcription subunit 20 (Protein from yeast)
- Rna polymerase ii transcription factor b subunit 2 (Protein from yeast)
- Mediator of rna polymerase ii transcription subunit 10 (Protein from yeast)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc 1 (Protein from yeast)
- Dna-directed rna polymerase ii subunit rpb9 (Protein from yeast)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc 4 (Protein from yeast)
- 108 bp his4 promoter fragment (DNA from Saccharomyces cerevisiae)
- Mediator of rna polymerase ii transcription subunit 21 (Protein from yeast)
- Transcription initiation factor iia large subunit (Protein from Saccharomyces cerevisiae)
- Rna polymerase ii transcription factor b subunit 3 (Protein from yeast)
- Dna-directed rna polymerase ii subunit rpb7 (Protein from yeast)
- Dna repair helicase rad3 (Protein from yeast)
- Mediator of rna polymerase ii transcription subunit 9 (Protein from yeast)
- Transcription factor tfiie subunit tfa1 (Protein from Saccharomyces cerevisiae)
- Mediator of rna polymerase ii transcription subunit 31 (Protein from yeast)
- Transcription initiation factor iia subunit 2 (Protein from Saccharomyces cerevisiae)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc3 (Protein from yeast)
- Dna repair helicase rad25 (Protein from yeast)
- Dna-directed rna polymerase ii subunit rpb4 (Protein from yeast)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc 2 (Protein from yeast)
- Mediator of rna polymerase ii transcription subunit 6 (Protein from yeast)
- Transcription initiation factor iif subunit gamma (Protein from yeast)
- Mediator of rna polymerase ii transcription subunit 17 (Protein from yeast)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc5 (Protein from yeast)
- Rna polymerase ii transcription factor b subunit 1 (Protein from yeast)
- Dna-directed rna polymerase ii subunit rpb3 (Protein from yeast)
- Mediator of rna polymerase ii transcription subunit 8 (Protein from yeast)
- Tata-box-binding protein (Protein from Saccharomyces cerevisiae)
- Dna-directed rna polymerase ii subunit rpb2 (Protein from yeast)
- Mediator of rna polymerase ii transcription subunit 11 (Protein from yeast)
- Serine/threonine-protein kinase kin28 (Protein from yeast)
- Mediator of rna polymerase ii transcription subunit 16 (Protein from yeast)
- Suppressor of stem-loop protein 1 (Protein from yeast)
- Mediator of rna polymerase ii transcription subunit 3 (Protein from yeast)
- Rna polymerase ii transcription factor b subunit 5 (Protein from yeast)
- Transcription factor tfiie subunit tfa2 (Protein from Saccharomyces cerevisiae)
- Transcription initiation factor iif subunit beta (Protein from yeast)
- Dna-directed rna polymerase ii subunit rpb11 (Protein from yeast)
- Mediator of rna polymerase ii transcription subunit 5 (Protein from yeast)
- Mediator of rna polymerase ii transcription subunit 18 (Protein from yeast)
- Mediator of rna polymerase ii transcription subunit 19 (Protein from yeast)
- Mediator of rna polymerase ii transcription subunit 15 (Protein from yeast)
- Mediator of rna polymerase ii transcription subunit 7 (Protein from yeast)
- Mediator of rna polymerase ii transcription subunit 22 (Protein from yeast)
- Mediator of rna polymerase ii transcription subunit 14 (Protein from yeast)
- Rna polymerase ii transcription factor b subunit 4 (Protein from yeast)
- Mediator of rna polymerase ii transcription subunit 2 (Protein from yeast)
- Transcription initiation factor iib (Protein from Saccharomyces cerevisiae)
- Dna-directed rna polymerase ii subunit rpb1 (Protein from yeast)
- Cyclin ccl1 (Protein from yeast)