Close-ring hexamer of the substrate-bound Lon protease with an S678A mutation
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 7ypk
Q-score: 0.231
Li S, Hsieh KY, Kuo CI, Lin TC, Lee SH, Chen YR, Wang CH, Ho MR, Ting SY, Zhang K, Chang CI
Nat Commun (2023) 14 pp. 7340-7340 [ Pubmed: 37957149 DOI: doi:10.1038/s41467-023-43035-2 ]
- Alpha-s1-casein (1 kDa, Protein from Bos taurus)
- Close-ring hexamer of mtalon-s678a in a substrate-engaged state (530 kDa, Complex from Bos taurus)
- Lon protease (88 kDa, Protein from Meiothermus taiwanensis)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Alpha-s1-casein (Complex from Meiothermus taiwanensis)
- Close-ring hexamer of lon protease (Complex)
Resolution: 2.4 Å
EM Method: Single-particle
Fitted PDBs: 7fd4
Q-score: 0.4
Li S, Hsieh KY, Kuo CI, Lee SH, Pintilie GD, Zhang K, Chang CI
Sci Adv (2021) 7 pp. eabj7835-eabj7835 [ Pubmed: 34652947 DOI: doi:10.1126/sciadv.abj7835 ]
- Alpha-s1-casein (1 kDa, Protein from Bos taurus)
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Alpha-s1-casein (Complex)
- Lon protease (Complex from Bos taurus)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- A complete three-dimensional structure of the lon protease translocating a protein substrate (conformation 1) (370 kDa, Complex from Meiothermus taiwanensis)
- Lon protease (88 kDa, Protein from Meiothermus taiwanensis)
- N-[(1r)-1-(dihydroxyboranyl)-2-phenylethyl]-nalpha-(pyrazin-2-ylcarbonyl)-l-phenylalaninamide (418 Da, Ligand)
Resolution: 2.4 Å
EM Method: Single-particle
Fitted PDBs: 7fd5
Q-score: 0.397
Li S, Hsieh KY, Kuo CI, Lee SH, Pintilie GD, Zhang K, Chang CI
Sci Adv (2021) 7 pp. eabj7835-eabj7835 [ Pubmed: 34652947 DOI: doi:10.1126/sciadv.abj7835 ]
- Alpha-s1-casein (1 kDa, Protein from Bos taurus)
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Alpha-s1-casein (Complex)
- Lon protease (Complex from Bos taurus)
- A complete three-dimensional structure of the lon protease translocating a protein substrate (conformation 2) (370 kDa, Complex from Meiothermus taiwanensis)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Lon protease (88 kDa, Protein from Meiothermus taiwanensis)
- N-[(1r)-1-(dihydroxyboranyl)-2-phenylethyl]-nalpha-(pyrazin-2-ylcarbonyl)-l-phenylalaninamide (418 Da, Ligand)
CryoEM map of the hyperactive ClpB mutant K476C, bound to casein, pre-state
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 6oax
Q-score: 0.5
Rizo AN, Lin J, Gates SN, Tse E, Bart SM, Castellano LM, DiMaio F, Shorter J, Southworth DR
Nat Commun (2019) 10 pp. 2393-2393 [ DOI: doi:10.1038/s41467-019-10150-y Pubmed: 31160557 ]
- Hyperactive disaggregase clpb (97 kDa, Protein from Escherichia coli K-12)
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Hyperactive clpb mutant k476c (Complex from Escherichia coli K-12)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Alpha-s1-casein (2 kDa, Protein from Bos taurus)
- Hyperactive clpb mutant k476c bound to casein (Complex)
- Alpha-s1-casein (Complex from Bos taurus)
CryoEM map of the hyperactive ClpB mutant K476C, bound to casein, post-state
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 6oay
Q-score: 0.437
Rizo AN, Lin J, Gates SN, Tse E, Bart SM, Castellano LM, DiMaio F, Shorter J, Southworth DR
Nat Commun (2019) 10 pp. 2393-2393 [ Pubmed: 31160557 DOI: doi:10.1038/s41467-019-10150-y ]
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Alpha-s1-casein (2 kDa, Protein from Bos taurus)
- Hyperactive disaggregase clpb (97 kDa, Protein from Escherichia coli K-12)
- Alpha-s1-casein (Complex from Bos taurus)
- Hyperactive clpb mutant k476c bound to casein (Complex)
- Hyperactive clpb mutant k476c (Complex from Escherichia coli K-12)
- Adenosine-5'-diphosphate (427 Da, Ligand)
CryoEM focus classification map of the hyperactive ClpB mutant K476C, bound to casein, NTD-trimer
Resolution: 4.1 Å
EM Method: Single-particle
Fitted PDBs: 6og3
Q-score: 0.306
Rizo AN, Lin J, Gates SN, Tse E, Bart SM, Castellano LM, DiMaio F, Shorter J, Southworth DR
Nat Commun (2019) 10 pp. 2393-2393 [ Pubmed: 31160557 DOI: doi:10.1038/s41467-019-10150-y ]
- Alpha s1-casein (783 Da, Protein from Bos taurus)
- Hyperactive clpb mutant k476c_ntd trimer (Complex from Escherichia coli K-12)
- Alpha-s1-casein (Complex from Bos taurus)
- Hyperactive clpb mutant k476c bound to casein (Complex)
- Hyperactive disaggregase clpb (96 kDa, Protein from Escherichia coli K-12)
S. cerevisiae Hsp104:casein complex, Middle Domain Conformation
Resolution: 6.7 Å
EM Method: Single-particle
Fitted PDBs: 5vy9
Q-score: 0.161
Gates SN, Yokom AL, Lin J, Jackrel ME, Rizo AN, Kendsersky NM, Buell CE, Sweeny EA, Mack KL, Chuang E, Torrente MP, Su M, Shorter J, Southworth DR
Science (2017) 357 pp. 273-279 [ Pubmed: 28619716 DOI: doi:10.1126/science.aan1052 ]
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Hsp104 (Complex)
- Heat shock protein 104 (102 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- S. cerevisiae hsp104:casein complex, middle domain conformation (Complex from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Casein (Complex from Bos taurus)
- Alpha-s1-casein (2 kDa, Protein from Bos taurus)
S. cerevisiae Hsp104:casein complex, Extended Conformation
Resolution: 4.0 Å
EM Method: Single-particle
Fitted PDBs: 5vya
Q-score: 0.313
Gates SN, Yokom AL, Lin J, Jackrel ME, Rizo AN, Kendsersky NM, Buell CE, Sweeny EA, Mack KL, Chuang E, Torrente MP, Su M, Shorter J, Southworth DR
Science (2017) 357 pp. 273-279 [ DOI: doi:10.1126/science.aan1052 Pubmed: 28619716 ]
- Alpha-s1-casein (2 kDa, Protein from Bovine)
- S. cerevisiae hsp104:casein complex, extended conformation (Complex from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Heat shock protein 104 (102 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)