Family M30
Summary for family M30
| Name | Peptidase family M30 (hyicolysin family) |
|---|
| Family type peptidase | M30.001 - hyicolysin (Staphylococcus hyicus), MEROPS Accession MER0001182 (peptidase unit: 102-438) |
| Content of family | Family M30 contains endopeptidases. |
| History |
Identifier created: Methods Enzymol. 248:183-228 (1995)
Only hyicolysin (M30.001), the peptidase from Staphylococcus hyicus, has been characterized (for a review see Götz, 2004). |
| Catalytic type | Metallo |
| Active site residues | H242 E243 H246 E269 |
| Active site | Hyicolysin is a metallopeptidase with a single zinc ion, liganded by two histidines and a glutamate. The histidines occur in an HEXXH motif, in which the glutamate is believed to be a catalytic residue. |
| Activities and specificities | Hyicolysin is active between pH 7.4 and 8.5. The peptidase is able to cleave azocasein and gelatin, but not elastin, FA-Gly-Leu-amide, a synthetic substrate for thermolysin (M04.001) nor FA-Leu-Gly-Pro-Ala, a synthetic substrate for collagenase 1 (M10.001). |
| Inhibitors | Hyicolysin is inhibited by the metal chelators EDTA, EGTA and 1,10-phenanthroline. Only zinc and cobalt ions can reactivate the enzyme. Unlike thermolysin, hyicolysin is not inhibited by phosphoramidon. |
| Molecular structure | No tertiary structure has been determined for any member of the family, but on the basis of the presence of the HEXXH zinc-binding motif, coupled with Glu as the third zinc ligand, family M30 is included in clan MA. |
| Clan | MA |
| Subclan | MA(E) |
| Basis of clan assignment | Predicted active site residues for members of this family and thermolysin, the type example for clan MA, occur in the motif HEXXH |
|
Distribution of family
|
Bacteria |
details |
|
|
|
Archaea |
details |
|
|
|
Protozoa |
- |
|
|
|
Fungi |
- |
|
|
|
Plants |
- |
|
|
|
Animals |
details |
|
|
|
Viruses |
- |
|
| Biological functions | Physiological substrates are unknown. Hyicolysin is synthesized as a precursor with an N-terminal signal peptide followed by a propeptide. Maturation is assumed to be by autolysis, and mature hyicolysin is secreted to the extracellular medium. |
| Pharmaceutical and biotech relevance | Staphylococcus hyicus infects pigs, cattle and chickens. In pigs it is associated with exudative dermatitis, necrosis of the tips of the ears, and tail-biting. Because the hyicolysin gene is present in isolates from infected animals, it is assumed to have a role in pathology and to be of agricultural and veterinary importance. |
| Statistics for family M30 | Sequences: | 468 |
| Identifiers: | 1 |
| Identifiers with PDB entries: | 0 |
| Downloadable files |
Sequence library (FastA format) |
| Sequence alignment (FastA format) |
| Phylogenetic tree (Newick format) |
