Family M60
Summary for family M60
| Name | Peptidase family M60 (enhancin family) |
|---|
| Family type peptidase | M60.001 - enhancin (Lymantria dispar nucleopolyhedrovirus), MEROPS Accession MER0014453 (peptidase unit: 1-782) |
| Content of family | Peptidase family M60 contains metalloendopeptidases. |
| History |
Identifier created: MEROPS 5.7 (17 December 2001)
Enhancins are peptidases from granulosis viruses that affect Lepidoptera. Enhancins increase the potency of viral infection. |
| Catalytic type | Metallo |
| Active site residues | H242 E243 H246 |
| Active site | Members of family M60 contain two metal binding ligands and a single catalytic residue found within an HEXXH motif (see the Alignment). The third metal ligand is not currently known. |
| Activities and specificities | Currently, the only identified substrate for enhancin is insect intestinal mucin, which is degraded into a number of fragments (Wang & Granados, 1997). The peptidase is active between pH 8.5 and 10.5. |
| Inhibitors | Enhancin activity is inhibited by metal chelators such as EDTA, EGTA and 1,10-phenanthroline. |
| Molecular structure | No tertiary structure is available. |
| Clan | MA |
| Subclan | MA(E) |
| Basis of clan assignment | Active site residues for members of this family and thermolysin, the type example for clan MA, occur in the motif HEXXH |
| Biological functions | Enhancin is incorporated into viral occlusion bodies which are ingested by the host. The occlusion bodies are broken down in the midgut of the host and the enhancin released. The enhancin digests the intestinal mucin in the midgut peritrophic membrane allowing the passage of the virus into the midgut epithelial cells and increased infection (Wang & Granados, 2004). |
| Pharmaceutical and biotech relevance | There is potential for the virus to be used as a method of pest control. |
| Statistics for family M60 | Sequences: | 83 |
| Identifiers: | 6 |
| Identifiers with PDB entries: | 1 |
| Downloadable files |
Sequence library (FastA format) |
| Sequence alignment (FastA format) |
| Phylogenetic tree (Newick format) |
