Family N2
Summary for family N2
| Family type peptidase | N02.001 - tetravirus coat protein (Nudaurelia capensis omega virus), MEROPS Accession MER0004241 (peptidase unit: 1-644) |
| Content of family | Peptidase family N2 contains only the endopeptidase of tetraviruses. |
| History |
Identifier created: MEROPS 9.3 (7 September 2010)
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| Catalytic type | Asparagine |
| Active site residues | E103 N570 |
| Active site | The residues that form the catalytic dyad in the active site are Glu103 and Asn570. The cleavage mediated by the enzyme is an autolytic one, and the catalytic Asn is also the P1 residue in the cleavage site. |
| Activities and specificities | The only known activity of family N2 is an autolytic cleavage of Asn570 Phe571 releasing a 74-residue fragment from the C-terminus of the coat protein. This cleavage occurs during the late stages of virion assembly. Heterologous expression of the coat protein gene of tetravirus in a baculovirus system results in the spontaneous assembly of virus-like particles (VLPs) that remain uncleaved when purified at neutral pH (Taylor et al., 2005). |
| Molecular structure | The tertiary structure of the Nuradelia capensis omega virus has been solved (Munshi et al., 1996). The fold of the peptidases of family N2 is similar to that of the known structures from family N1, and the families are therefore grouped together in clan NA. |
| Clan | NA |
| Basis of clan assignment | Protein fold of the peptidase unit for members of this family that of nodavirus endopeptidase, the type example for clan AB. |
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Distribution of family
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Bacteria |
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Archaea |
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Protozoa |
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Fungi |
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Plants |
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Animals |
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Viruses |
details |
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