$VAR1 = undef;

Summary for peptidase S01.151: trypsin 1

Summary Gene structure Alignment Tree Sequences Sequence features Distribution Structure Literature Substrates Pharma

 

Names
MEROPS Nametrypsin 1
Other namespseudotrypsin, psi-trypsin, trypsin 7 (Mus musculus), trypsin III (cationic) (Rattus norvegicus), trypsin 1 (Sus scrofa), trypsin 1 (tetrapod, non-Homo sapiens)
Domain architecture
MEROPS Classification
Classification Clan PA >> Subclan PA(S) >> Family S1 >> Subfamily A >> S01.151
Holotypetrypsin 1 (Bos taurus), Uniprot accession P00760 (peptidase unit: 24-246), MERNUM MER0000024
History Identifier created: Handbook of Proteolytic Enzymes (1998) Academic Press, London.
Activity
Catalytic typeSerine
PeplistIncluded in the Peplist with identifier PL00281
NC-IUBMBSubclass 3.4 (Peptidases) >> Sub-subclass 3.4.21 (Serine endopeptidases) >> Peptidase 3.4.21.4
EnzymologyBRENDA database
Proteolytic eventsCutDB database (29 cleavages)
PhysiologyMajor role in intestinal digestion of food proteins in mammals, and also in the activation of zymogens of other digestive peptidases.
Pathways KEGGInfluenza A
KEGGNeuroactive ligand-receptor interaction
KEGGPancreatic secretion
KEGGProtein digestion and absorption
Other databases WIKIPEDIAhttp://en.wikipedia.org/wiki/Trypsin_1
Cleavage site specificity Explanations of how to interpret the following cleavage site sequence logo and specificity matrix can be found here.
Cleavage pattern-/-/-/KRScissile bond-/-/-/- (based on 22992 cleavages)
weblogo
Specificity matrix
 
Amino acid P4 P3 P2 P1 P1' P2' P3' P4'
Gly 1474 1663 1796 35 1589 1752 1736 1489
Pro 1334 1017 1424 5 58 1082 1369 1475
Ala 1906 1925 1965 27 2164 2018 1918 1765
Val 1427 1680 1579 5 1971 1856 1703 1615
Leu 2099 2687 2500 18 2496 2929 2093 2135
Ile 1259 1409 1321 8 1594 1571 1289 1317
Met 467 545 507 5 611 566 450 453
Phe 779 986 916 10 916 984 979 803
Tyr 591 713 675 10 734 696 617 524
Trp 184 214 235 0 204 212 161 237
Ser 1434 1412 1480 9 1508 1385 1503 1545
Thr 1181 1258 1151 3 1532 1253 1310 1356
Cys 256 307 305 5 231 303 325 284
Asn 1009 900 913 8 1018 789 878 887
Gln 1121 1035 1150 9 908 1009 1183 1225
Asp 1605 1201 875 0 1276 1106 1526 1662
Glu 2153 1595 1829 1 1690 1195 2182 2434
Lys 1316 1250 1250 12979 1215 1115 789 740
Arg 863 720 706 9851 703 688 510 521
His 454 432 387 4 537 433 391 412
Specificity from combinatorial peptides
 
Organism comment P4 P3 P2 P1 P1' P2' P3' P4' optimal substrate fluorophore or acceptor-donor pair Reference
Sus scrofa recombinant broad broad broad R - - - - bbbR AMC Furlong et al., 2002
Homo sapiens recombinant nd nd nd R - - - - R ACC Harris et al., 2000
Bos taurus wild type - L P R H L P - xLPR+HLPx Abz-Dnp Alves et al., 2007
Homo sapiens recombinant Q K G R - - - - QKGR+xxxx Abz-Tyr(NO2) Janssen et al., 2004
Homo sapiens recombinant - M/K/Q R/K - R/S V/I/R G/P/S/D - xMRx+RVGx Abz-EDDnp Bianchini et al., 2002
Bos taurus wild type - K A/broad K - - - - xKAK+xxxx ACC-Dabcyl Sun et al., 2007
Mouse genetics
Gene symbol Position Megabases Ensembl Entrez gene MGI
2210010C04Rik 6:B1 ENSMUSG00000029882 67373 MGI:1914623