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CRYSTAL STRUCTURES OF PHOSPHONOACETAMIDE LIGATED T AND PHOSPHONOACETAMIDE AND MALONATE LIGATED R STATES OF ASPARTATE CARBAMOYLTRANSFERASE AT 2.8-ANGSTROMS RESOLUTION AND NEUTRAL P*H

X-ray diffraction
2.8Å resolution
PDB entry 1at1 coloured by chain, front view.
PDB entry 1at1 coloured by chain, side view.
PDB entry 1at1 coloured by chain, top view.
Source organism: Escherichia coli
Assembly composition:
hetero dodecamer (preferred)
Entry contents: 2 distinct polypeptide molecules

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Aspartate carbamoyltransferase complex
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Multimeric state: hetero dodecamer
Accessible surface area: 100892.29 Å2
Buried surface area: 31424.77 Å2
Dissociation area: 7,366.32 Å2
Dissociation energy (ΔGdiss): 29.62 kcal/mol
Dissociation entropy (TΔSdiss): 94.54 kcal/mol
Symmetry number: 6
PDBe Complex ID: PDB-CPX-141554
Complex Portal ID: CPX-3091

Macromolecules

Aspartate carbamoyltransferase catalytic subunit
Chains: A, C
Length: 310 amino acids
Theoretical weight: 34.34 KDa
Source organism: Escherichia coli
Expression system: Not provided
UniProt:
  • Canonical: P0A786 (Residues: 2-311; Coverage: 100%)
Gene names: JW4204, b4245, pyrB
Pfam:
InterPro:
CATH: Aspartate/ornithine carbamoyltransferase
SCOP: Aspartate/ornithine carbamoyltransferase
Aspartate carbamoyltransferase catalytic subunit in PDB entry 1at1, assembly 1, front view.
Aspartate carbamoyltransferase catalytic subunit in PDB entry 1at1, assembly 1, side view.
Aspartate carbamoyltransferase catalytic subunit in PDB entry 1at1, assembly 1, top view.
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Aspartate carbamoyltransferase regulatory chain
Chains: B, D
Length: 153 amino acids
Theoretical weight: 17.07 KDa
Source organism: Escherichia coli
Expression system: Not provided
UniProt:
  • Canonical: P0A7F3 (Residues: 1-153; Coverage: 100%)
Gene names: JW4203, b4244, pyrI
Pfam:
InterPro:
CATH:
SCOP:
Aspartate carbamoyltransferase regulatory chain in PDB entry 1at1, assembly 1, front view.
Aspartate carbamoyltransferase regulatory chain in PDB entry 1at1, assembly 1, side view.
Aspartate carbamoyltransferase regulatory chain in PDB entry 1at1, assembly 1, top view.
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