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CRYSTALLOGRAPHIC ANALYSES OF SITE-DIRECTED MUTANTS OF THE PHOTOSYNTHETIC REACTION CENTER FROM RHODOBACTER SPHAEROIDES

X-ray diffraction
3Å resolution
PDB entry 1pss coloured by chain, front view.
PDB entry 1pss coloured by chain, side view.
PDB entry 1pss coloured by chain, top view.
Source organism: Cereibacter sphaeroides
Assembly composition:
hetero trimer (preferred)
Entry contents: 3 distinct polypeptide molecules

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Reaction Centre
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Multimeric state: hetero trimer
Accessible surface area: 30155.86 Å2
Buried surface area: 30557.83 Å2
Dissociation area: 3,764.65 Å2
Dissociation energy (ΔGdiss): 41.18 kcal/mol
Dissociation entropy (TΔSdiss): 14.47 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-142934

Macromolecules

Reaction center protein L chain
Chain: L
Length: 266 amino acids
Theoretical weight: 29.8 KDa
Source organism: Cereibacter sphaeroides
Expression system: Not provided
UniProt:
  • Canonical: P0C0Y8 (Residues: 6-271; Coverage: 94%)
Gene name: pufL
Pfam: Photosynthetic reaction centre protein
InterPro:
CATH: Photosystem II protein D1-like
SCOP: Bacterial photosystem II reaction centre, L and M subunits
Reaction center protein L chain in PDB entry 1pss, assembly 1, front view.
Reaction center protein L chain in PDB entry 1pss, assembly 1, side view.
Reaction center protein L chain in PDB entry 1pss, assembly 1, top view.
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Reaction center protein M chain
Chain: M
Length: 296 amino acids
Theoretical weight: 33.21 KDa
Source organism: Cereibacter sphaeroides
Expression system: Not provided
UniProt:
  • Canonical: P0C0Y9 (Residues: 7-302; Coverage: 96%)
Gene name: pufM
Pfam: Photosynthetic reaction centre protein
InterPro:
CATH: Photosystem II protein D1-like
SCOP: Bacterial photosystem II reaction centre, L and M subunits
Reaction center protein M chain in PDB entry 1pss, assembly 1, front view.
Reaction center protein M chain in PDB entry 1pss, assembly 1, side view.
Reaction center protein M chain in PDB entry 1pss, assembly 1, top view.
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Reaction center protein H chain
Chain: H
Length: 237 amino acids
Theoretical weight: 25.69 KDa
Source organism: Cereibacter sphaeroides
Expression system: Not provided
UniProt:
  • Canonical: P0C0Y7 (Residues: 12-248; Coverage: 91%)
Gene name: puhA
Pfam:
InterPro:
CATH:
SCOP:
Reaction center protein H chain in PDB entry 1pss, assembly 1, front view.
Reaction center protein H chain in PDB entry 1pss, assembly 1, side view.
Reaction center protein H chain in PDB entry 1pss, assembly 1, top view.
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