Structure analysis

The structure of the transition state analogue "RAA" bound to the large ribosomal subunit of haloarcula marismortui

X-ray diffraction
2.6Å resolution
Source organism: Haloarcula marismortui
Assembly composition:
hetero 32-mer (preferred)
Entry contents: 29 distinct polypeptide molecules
3 distinct RNA molecules

Assemblies

Assembly 1 (preferred)
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Multimeric state: hetero 32-mer
Accessible surface area: 444833.77 Å2
Buried surface area: 213649.49 Å2
Dissociation area: 484.11 Å2
Dissociation energy (ΔGdiss): 1.08 kcal/mol
Dissociation entropy (TΔSdiss): 9.67 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-145553

Macromolecules

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Chain: N
Length: 187 amino acids
Theoretical weight: 20.64 KDa
Source organism: Haloarcula marismortui
UniProt:
  • Canonical: P14123 (Residues: 1-187; Coverage: 100%)
Gene names: rpl18, rrnAC1593
Pfam: Ribosomal large subunit proteins 60S L5, and 50S L18
InterPro: Large ribosomal subunit protein uL18 eukaryotic/archaeal
CATH: Nucleotidyltransferase; domain 5
SCOP: Ribosomal protein L18 and S11

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Chain: V
Length: 71 amino acids
Theoretical weight: 7.89 KDa
Source organism: Haloarcula marismortui
UniProt:
  • Canonical: P10971 (Residues: 1-71; Coverage: 100%)
Gene names: rpl29, rrnAC1604
Pfam: Ribosomal L29 protein
InterPro:
CATH: Helix Hairpins
SCOP: Ribosomal protein L29 (L29p)

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Chain: X
Length: 92 amino acids
Theoretical weight: 10.38 KDa
Source organism: Haloarcula marismortui
UniProt:
  • Canonical: P18138 (Residues: 1-92; Coverage: 100%)
Gene names: rpl31e, rrnAC3113
Pfam: Ribosomal protein L31e
InterPro:
CATH: Ribosomal Protein L31e; Chain: W;
SCOP: Ribosomal protein L31e

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Chain: Z
Length: 83 amino acids
Theoretical weight: 9.41 KDa
Source organism: Haloarcula marismortui
UniProt:
  • Canonical: P60619 (Residues: 10-92; Coverage: 90%)
Gene names: rpl37ae, rrnAC1669
Pfam: Ribosomal L37ae protein family
InterPro:
CATH: N-terminal domain of TfIIb
SCOP: Ribosomal protein L37ae

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Chain: 3
Length: 92 amino acids
Theoretical weight: 10.82 KDa
Source organism: Haloarcula marismortui
UniProt:
  • Canonical: P32411 (Residues: 1-92; Coverage: 100%)
Gene names: rpl44e, rrnAC3514
Pfam: Ribosomal protein L44
InterPro:
CATH: Nuclear Transport Factor 2; Chain: A,
SCOP: Ribosomal protein L44e

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Name: 23S ribosomal RNA
Representative chains: 0
Length: 2922 nucleotides
Theoretical weight: 946.09 KDa
Rfam: Archaeal large subunit ribosomal RNA

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Name: 5S ribosomal RNA
Representative chains: 9
Length: 122 nucleotides
Theoretical weight: 39.32 KDa
Rfam: 5S ribosomal RNA

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Name: 5'-D(*(DC)P*(DC)P*(5AA)P*(2OP)P*(PO2)P*AP*C*C)-3')
Representative chains: 4
Length: 8 nucleotides
Theoretical weight: 1.87 KDa

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