› 2c3z

X-ray diffraction
2.8Å resolution

Crystal structure of a truncated variant of indole-3-glycerol phosphate synthase from Sulfolobus solfataricus

Released:
DOI: 10.2210/pdb2c3z/pdb
PDB entry 2c3z coloured by chain, front view.
PDB entry 2c3z coloured by chain, side view.
PDB entry 2c3z coloured by chain, top view.
Source organism: Saccharolobus solfataricus
Primary publication:
Role of the N-terminal extension of the (betaalpha)8-barrel enzyme indole-3-glycerol phosphate synthase for its fold, stability, and catalytic activity.
Schneider B, Knöchel T, Darimont B, Hennig M, Dietrich S, Babinger K, Kirschner K, Sterner R
Biochemistry 44 16405-12 (2005)
PMID: 16342933

Function and Biology Details

Reaction catalysed: 
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate = 1-C-(3-indolyl)-glycerol 3-phosphate + CO(2) + H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170286 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Indole-3-glycerol phosphate synthase Chain: A
Molecule details ›
Chain: A
Length: 222 amino acids
Theoretical weight: 25.48 KDa
Source organism: Saccharolobus solfataricus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q06121 (Residues: 27-248; Coverage: 90%)
Gene names: SSO0895, trpC
Sequence domains: Indole-3-glycerol phosphate synthase
Structure domains: Aldolase class I

Ligands and Environments

1 bound ligand:
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P212121
Unit cell:
a: 39.2Å b: 61.4Å c: 80.4Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.18 0.18 not available
Expression system: Escherichia coli

Quick links

Citations

7 citation in other articles

A novel genetic system for recombinant protein secretion in the Antarctic Pseudoalteromonas haloplanktis TAC125.
Cusano et al. (2006)
6 more

11 mentions without citation

Mapping the structure of folding cores in TIM barrel proteins by hydrogen exchange mass spectrometry: the roles of motif and sequence for the indole-3-glycerol phosphate synthase from Sulfolobus solfataricus.
Gu et al. (2007)
10 more