3w9c

X-ray diffraction
2.5Å resolution

Crystal structure of the electron transfer complex of cytochrome p450cam with putidaredoxin

Released:

Function and Biology Details

Reaction catalysed:
(+)-camphor + reduced putidaredoxin + O(2) = (+)-exo-5-hydroxycamphor + oxidized putidaredoxin + H(2)O
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-132315 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Camphor 5-monooxygenase Chain: A
Molecule details ›
Chain: A
Length: 416 amino acids
Theoretical weight: 46.66 KDa
Source organism: Pseudomonas putida
Expression system: Escherichia coli
UniProt:
  • Canonical: P00183 (Residues: 2-415; Coverage: 100%)
Gene names: camC, cyp101
Sequence domains: Cytochrome P450
Structure domains: Cytochrome P450
Putidaredoxin Chain: B
Molecule details ›
Chain: B
Length: 108 amino acids
Theoretical weight: 11.6 KDa
Source organism: Pseudomonas putida
Expression system: Escherichia coli
UniProt:
  • Canonical: P00259 (Residues: 2-107; Coverage: 99%)
Gene name: camB
Sequence domains: 2Fe-2S iron-sulfur cluster binding domain
Structure domains: Ubiquitin-like (UB roll)

Ligands and Environments


Cofactor: Ligand HEM 1 x HEM
3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: C2
Unit cell:
a: 101.717Å b: 77.991Å c: 60.016Å
α: 90° β: 95.57° γ: 90°
R-values:
R R work R free
0.188 0.185 0.251
Expression system: Escherichia coli