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M. jannaschii Nep1 complexed with S-adenosyl-homocysteine

X-ray diffraction
2.15Å resolution
PDB entry 3bbd coloured by chain, front view.
PDB entry 3bbd coloured by chain, side view.
PDB entry 3bbd coloured by chain, top view.
Source organism: Methanocaldococcus jannaschii
Assembly composition:
homo dimer (preferred)
Entry contents: 1 distinct polypeptide molecule

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Ribosomal RNA small subunit methyltransferase Nep1
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Multimeric state: homo dimer
Accessible surface area: 19157.45 Å2
Buried surface area: 4382.8 Å2
Dissociation area: 1,506.35 Å2
Dissociation energy (ΔGdiss): 5.8 kcal/mol
Dissociation entropy (TΔSdiss): 13.01 kcal/mol
Symmetry number: 2
PDBe Complex ID: PDB-CPX-176507

Macromolecules

Ribosomal RNA small subunit methyltransferase Nep1
Chains: A, B
Length: 205 amino acids
Theoretical weight: 24.45 KDa
Source organism: Methanocaldococcus jannaschii
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q57977 (Residues: 1-205; Coverage: 100%)
Gene names: MJ0557, nep1
Pfam: EMG1/NEP1 methyltransferase
InterPro:
CATH: Alpha/beta knot
SCOP: EMG1/NEP1-like
Ribosomal RNA small subunit methyltransferase Nep1 in PDB entry 3bbd, assembly 1, front view.
Ribosomal RNA small subunit methyltransferase Nep1 in PDB entry 3bbd, assembly 1, side view.
Ribosomal RNA small subunit methyltransferase Nep1 in PDB entry 3bbd, assembly 1, top view.
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