Structure analysis

The X-ray crystal structure of RNA polymerase from Archaea

X-ray diffraction
3.4Å resolution
Assembly composition:
hetero tridecamer (preferred)
Entry contents: 13 distinct polypeptide molecules

Assemblies

Assembly 1 (preferred)
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Multimeric state: hetero tridecamer
Accessible surface area: 137238.52 Å2
Buried surface area: 64518.16 Å2
Dissociation area: 1,203.23 Å2
Dissociation energy (ΔGdiss): -6.29 kcal/mol
Dissociation entropy (TΔSdiss): 11.21 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-157657
Assembly 2
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Multimeric state: hetero tridecamer
Accessible surface area: 137878.61 Å2
Buried surface area: 63898.95 Å2
Dissociation area: 983.42 Å2
Dissociation energy (ΔGdiss): -4.66 kcal/mol
Dissociation entropy (TΔSdiss): 11.15 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-157657

Macromolecules

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Chains: C, M
Length: 395 amino acids
Theoretical weight: 43.87 KDa
Source organism: Saccharolobus solfataricus P2
UniProt:
  • Canonical: P58192 (Residues: 1-392; Coverage: 100%)
Gene names: SSO0223, rpo1C, rpoA2
Pfam: RNA polymerase Rpb1, domain 5
InterPro:
CATH: DNA polymerase; domain 1

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Chains: F, R
Length: 113 amino acids
Theoretical weight: 12.83 KDa
Source organism: Saccharolobus solfataricus P2
UniProt:
  • Canonical: Q7LXK4 (Residues: 1-113; Coverage: 100%)
Gene names: SSO0751, rpo4, rpoF
Pfam: RNA polymerase Rpb4
InterPro:
CATH:

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Chains: G, S
Length: 132 amino acids
Theoretical weight: 14.99 KDa
Source organism: Saccharolobus solfataricus P2
UniProt:
  • Canonical: Q980L5 (Residues: 1-132; Coverage: 100%)
Gene names: SSO0277, rpo8, rpoG
Pfam: DNA-directed RNA polymerase, subunit G
InterPro:
CATH: Nucleic acid-binding proteins

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Chains: N, W
Length: 66 amino acids
Theoretical weight: 7.6 KDa
Source organism: Saccharolobus solfataricus P2
UniProt:
  • Canonical: Q980Z8 (Residues: 1-66; Coverage: 100%)
Gene names: SSO5140, rpo10, rpoN
Pfam: RNA polymerases N / 8 kDa subunit
InterPro:
CATH: Homeodomain-like

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Chains: Y, Z
Length: 104 amino acids
Theoretical weight: 12.2 KDa
Source organism: Saccharolobus solfataricus P2
UniProt:
  • Canonical: Q980B8 (Residues: 1-104; Coverage: 100%)
Gene names: SSO0396, rpo13
Pfam: RNA polymerase Rpo13 subunit HTH domain
InterPro: RNA polymerase Rpo13 subunit HTH domain
CATH: DNA Excision Repair, Uvrb; Chain A

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