Structure analysis

Crystal structure of the S1-cleaved Notch1 Negative Regulatory Region (NRR)

X-ray diffraction
3.2Å resolution
Source organism: Homo sapiens
Assemblies composition:
hetero dimer (preferred)
hetero tetramer
Entry contents: 2 distinct polypeptide molecules

Assemblies

Assembly 1 (preferred)
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Multimeric state: hetero dimer
Accessible surface area: 12109.21 Å2
Buried surface area: 6417.87 Å2
Dissociation area: 3,142.17 Å2
Dissociation energy (ΔGdiss): 55.17 kcal/mol
Dissociation entropy (TΔSdiss): 11.58 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-155480
Assembly 2
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Multimeric state: hetero dimer
Accessible surface area: 11570.59 Å2
Buried surface area: 6254.01 Å2
Dissociation area: 3,127 Å2
Dissociation energy (ΔGdiss): 56.05 kcal/mol
Dissociation entropy (TΔSdiss): 11.41 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-155480
Assembly 3
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Multimeric state: hetero tetramer
Accessible surface area: 22649.62 Å2
Buried surface area: 13729.04 Å2
Dissociation area: 520.55 Å2
Dissociation energy (ΔGdiss): 0.75 kcal/mol
Dissociation entropy (TΔSdiss): 12.63 kcal/mol
Symmetry number: 2
PDBe Complex ID: PDB-CPX-155485

Macromolecules

Chains: A, C
Length: 220 amino acids
Theoretical weight: 24.6 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P46531 (Residues: 1446-1664; Coverage: 9%)
Gene names: NOTCH1, TAN1
Pfam:
InterPro:
CATH: Ricin (A Subunit), domain 2

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Chains: B, D
Length: 69 amino acids
Theoretical weight: 7.38 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P46531 (Residues: 1665-1733; Coverage: 3%)
Gene names: NOTCH1, TAN1
Pfam: NOTCH protein
InterPro: Notch, NODP domain
CATH: Single alpha-helices involved in coiled-coils or other helix-helix interfaces

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