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Cryo-EM structure of GTPgammaS-microtubule co-polymerized with EB3 (merged dataset with and without kinesin bound)

Electron Microscopy
3.3Å resolution
PDB entry 3jak coloured by chain, front view.
PDB entry 3jak coloured by chain, side view.
PDB entry 3jak coloured by chain, top view.
Source organisms:
Assembly composition:
hetero tetradecamer (preferred)
Entry contents: 3 distinct polypeptide molecules

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Assemblies

Assembly 1 (preferred)
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Multimeric state: hetero tetradecamer
Accessible surface area: 178203.14 Å2
Buried surface area: 59397.5 Å2
Dissociation area: 6,900.14 Å2
Dissociation energy (ΔGdiss): -19.27 kcal/mol
Dissociation entropy (TΔSdiss): 61.82 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-135926

Macromolecules

Tubulin alpha-1B chain
Chains: A, C, E, J, K, L
Length: 451 amino acids
Theoretical weight: 50.2 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: Q2XVP4 (Residues: 1-451; Coverage: 100%)
Gene name: TUBA1B
Pfam:
InterPro:
CATH:
Tubulin alpha-1B chain in PDB entry 3jak, assembly 1, front view.
Tubulin alpha-1B chain in PDB entry 3jak, assembly 1, side view.
Tubulin alpha-1B chain in PDB entry 3jak, assembly 1, top view.
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Tubulin beta chain
Chains: B, D, F, G, H, I
Length: 445 amino acids
Theoretical weight: 49.91 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: P02554 (Residues: 1-445; Coverage: 100%)
Pfam:
InterPro:
CATH:
Tubulin beta chain in PDB entry 3jak, assembly 1, front view.
Tubulin beta chain in PDB entry 3jak, assembly 1, side view.
Tubulin beta chain in PDB entry 3jak, assembly 1, top view.
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Microtubule-associated protein RP/EB family member 3
Chains: M, N
Length: 203 amino acids
Theoretical weight: 22.87 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9UPY8 (Residues: 1-200; Coverage: 71%)
Gene name: MAPRE3
Pfam: Calponin homology (CH) domain
InterPro:
CATH: Calponin-like domain
Microtubule-associated protein RP/EB family member 3 in PDB entry 3jak, assembly 1, front view.
Microtubule-associated protein RP/EB family member 3 in PDB entry 3jak, assembly 1, side view.
Microtubule-associated protein RP/EB family member 3 in PDB entry 3jak, assembly 1, top view.
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